|
UPSI Digital Repository (UDRep)
|
|
| ||||||||||||||||
| Abstract : |
| Green synthesis approach was used to synthesis silver (Ag) nanoparticles. In this study, a one-step method was employed via hydrothermal technique. Samples are synthesized at different temperatures and times. All samples were characterized by Field Emission Scanning Electron Microscopy (FESEM). The morphology of the as-synthesized Ag samples are consists of nanoparticles and nanoplates with the diameter is in the range of 45 - 140 nm. The Ag nanoparticles were tested on Gram-Negative bacteria, Eschirichia coli (E.coli) which represent as an indicator for water pollution by using disc diffusion methods. Different concentrations of Ag nanoparticles were used to treat E.coli which is at 25 μg/ml, 50 μg/ml and 100 μg/ml respectively. The results show that for every samples, the inhibition zone of the E.coli increased as the concentration of Ag nanoparticles increased. Ag nanoparticles which synthesized at 100°C/ 8 hrs exhibits the most optimum inhibition zone for the growth of E.coli due to its smaller size and the triangular nanoplate shaped. The diameter of the inhibition zone is between 6.17 ± 0.03 to 8.03 ± 0.03 mm. |
| References |
1. Liau,S.Y.,Read,D.C.,Pugh,W.J.,Furr, J.R., Russell, A.D. Interaction of silver nitrate with readily identifiable groups: Relationship to the antibacterial action of silver ions (Open Access)(1997) Letters in Applied Microbiology, 25 (4), pp. 279-283. Cited 564 times. http://www3.interscience.wiley.com/journal/117997861/home doi: 10.1046/j.1472-765X.1997.00219.x 2. Ki, Y.Y., Jeong, H.B., Jae, H.P., Jungho, H. (2006) Journal Science of the Total Environment, 373, pp. 572-575. 3. Sondi, I., Salopek-Sondi, B. Silver nanoparticles as antimicrobial agent: A case study on E. coli as a model for Gram-negative bacteria (2004) Journal of Colloid and Interface Science, 275 (1), pp. 177-182. Cited 3417 times. doi: 10.1016/j.jcis.2004.02.012 4. Chudasama, B., Vala, A.K., Andhariya, N., Mehta, R.V., Upadhyay, R.V. Highly bacterial resistant silver nanoparticles: Synthesis and antibacterial activities (2010) Journal of Nanoparticle Research, 12 (5), pp. 1677-1685. Cited 76 times. doi: 10.1007/s11051-009-9845-1 5. Edberg, S.C., Rice, E.W., Karlin, R.J., Allen, M.J. Escherichia coli: The best biological drinking water indicator for public health protection (2000) Journal of Applied Microbiology Symposium Supplement, 88 (29), pp. 106s-116s. Cited 396 times. www.blacksci.co.uk/~cgilib/jnlpage.bin?Journal=jam&File=jam&Page=aims 6. Wani, I.A., Ganguly, A., Ahmed, J., Ahmad, T. Silver nanoparticles: Ultrasonic wave assisted synthesis, optical characterization and surface area studies (2011) Materials Letters, 65 (3), pp. 520-522. Cited 130 times. http://www.journals.elsevier.com/materials-letters/ doi: 10.1016/j.matlet.2010.11.003 7. RubenMorones-Ramirez, J., Winkler, J.A., Spina, C.S., Collins, J.J. Silver enhances antibiotic activity against gram-negative bacteria (2013) Science Translational Medicine, 5 (190), art. no. 190ra81. Cited 271 times. http://stm.sciencemag.org/content/5/190/190ra81.full.pdf doi: 10.1126/scitranslmed.3006276 8. Awwad, A.M., Salem, N.M., Abdeen, A.O. (2013) Int J Ind Chem, 4, p. 29. Cited 151 times. 9. Van Dong, P., Ha, C.H., Binh, L.T. (2012) Int Nano Lett, 2, p. 9. Cited 103 times. 10. Solioz, M., Odermatt, A. Copper and silver transport by CopB-ATPase in membrane vesicles of Enterococcus hirae (Open Access) (1995) Journal of Biological Chemistry, 270 (16), pp. 9217-9221. Cited 217 times. doi: 10.1074/jbc.270.16.9217 11. Kokkoris, M., Trapalis, C.C., Kossionides, S., Vlastou, R., Nsouli, B., Grötzschel, R., Spartalis, S., (...), Paradellis, Th. RBS and HIRBS studies of nanostructured AgSiO2 sol-gel thin coatings (2002) Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms, 188 (1-4), pp. 67-72. Cited 49 times. doi: 10.1016/S0168-583X(01)01020-5 12. Bell, R.A., Kramer, J.R. Structural chemistry and geochemistry of silver-sulfur compounds: Critical review (1999) Environmental Toxicology and Chemistry, 18 (1), pp. 9-22. Cited 155 times. doi: 10.1897/1551-5028(1999)018<0009:SCAGOS>2.3.CO;2 13. Behra, R., Sigg, L., Clift, M.J.D., Herzog, F., Minghetti, M., Johnston, B., Petri-Fink, A., (...), Rothen-Rutishauser, B. Bioavailability of silver nanoparticles and ions: From a chemical and biochemical perspective (Open Access) (2013) Journal of the Royal Society Interface, 10 (87), art. no. 20130396. Cited 161 times. http://rsif.royalsocietypublishing.org/content/10/87/20130396.full.pdf+html doi: 10.1098/rsif.2013.0396 14. Feng, Q.L., Wu, J., Chen, G.Q., Cui, F.Z., Kim, T.N., Kim, J.O. A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus (2000) Journal of Biomedical Materials Research, 52 (4), pp. 662-668. Cited 2464 times. doi: 10.1002/1097-4636(20001215)52:4<662::AID-JBM10>3.0.CO;2-3 |
| This material may be protected under Copyright Act which governs the making of photocopies or reproductions of copyrighted materials. You may use the digitized material for private study, scholarship, or research. |