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Type :article
Subject :Q Science (General)
ISSN :0094243X
Main Author :Komathi Sockalingam
Additional Authors :Rosmilah Misnan
Zailatul Hani Mohd Yadzir
Title :The effects of thermal treatments on protein profiles of Macrobrachium rosenbergii (giant river prawn)
Year of Publication :2017

Abstract :
Prawn allergy is certainly the most frequent cause of allergic reactions in countries where this crustacean is a popular dish of seafood. The aim of this study was to determine the protein profiles of giant river prawn which scientifically known as Macrobrachium rosenbergii. Raw and cooked extracts (boiled, steamed and fried) of prawn samples were prepared and then resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). 27 protein bands between 6 to 207 kDa were detected in the SDS-PAGE gel of raw extracts while boiled, steamed and fried extracts revealed fewer protein bands. Steamed and boiled prawns presented higher numbers of protein bands compared to fried prawn. A prominent heat-resistant band between 32 to 38 kDa was seen in all extracts, might hypothesized to be tropomyosin. Other prominent bands between 17 to 20 kDa were also seen in all treated prawn extracts while bands of 24 to 27 kDa were seen in steamed and boiled prawn extracts. These positions are consistent with the known shellfish allergens myosin light chain, sacroplasmic calcium binding protein and troponin C respectively. Several other heat-sensitive protein bands at various molecular weights were also not detected in boiled, steamed and fried extracts of this prawn. This study showed that M. rosenbergii contains numerous heat-sensitive and heat-resistant proteins, which may play an important role in prawn allergy.

References

1. Abdel Rahman, A.M., Kamath, S.D., Gagné, S., Lopata, A.L., Helleur, R. Comprehensive proteomics approach in characterizing and quantifying allergenic proteins from northern shrimp: Toward better occupational asthma prevention (2013) Journal of Proteome Research, 12 (2), pp. 647-656. Cited 32 times. doi: 10.1021/pr300755p 2. DeWitt, Å.M., Mattsson, L., Lauer, I., Reese, G., Lidholm, J. Recombinant tropomyosin from Penaeus aztecus (rPen a 1) for measurement of specific immunoglobulin E antibodies relevant in food allergy to crustaceans and other invertebrates (2004) Molecular Nutrition and Food Research, 48 (5), pp. 370-379. Cited 38 times. doi: 10.1002/mnfr.200400015 3. Opinion of the Scientific Panel on Dietetic Products, Nutrition and Allergies on a request from the Commission related to the evaluation of molluscs for labelling purposes (2006) The EFSA Journal, 327, pp. 1-25. Cited 18 times. EFSA. (2006). 4. Beyer, K., Morrow, E., Li, X.-M., Bardina, L., Bannon, G.A., Burks, A.W., Sampson, H.A. Effects of cooking methods on peanut allergenicity (2001) Journal of Allergy and Clinical Immunology, 107 (6), pp. 1077-1081. Cited 338 times. doi: 10.1067/mai.2001.115480 5. Lopata, A.L., Lehrer, S.B. New insights into seafood allergy (2009) Current Opinion in Allergy and Clinical Immunology, 9 (3), pp. 270-277. Cited 146 times. doi: 10.1097/ACI.0b013e32832b3e6f 6. Jeebhay,M.F.,Robins, T.G.,Lehrer, S.B.,Lopata, A.L. Occupational seafood allergy: A review (Open Access) (2001) Occupational and Environmental Medicine, 58 (9), pp. 553-562. Cited 179 times. doi: 10.1136/oem.58.9.553 7. Rosmilah, M., Shahnaz, M., Zailatul, H.M.Y., Noormalin, A., Normilah, I. Identification of tropomyosin and arginine kinase as major allergens of Portunus pelagicus (blue swimming crab)(2012) Tropical Biomedicine, 29 (3), pp. 467-478. Cited 26 times. http://www.msptm.org/files/467_-_478_Rosmilah_M.pdf 8. Netting, M., Donato, A., Makrides, M., Gold, M., Quinn, P., Penttila, I. Allergenicity of pasteurized whole raw Hen's egg compared with fresh whole raw Hen's egg (2015) Pediatric Allergy and Immunology, 26 (3), pp. 234-238. Cited 7 times. http://www.wiley.com/bw/journal.asp?ref=0905-6157 doi: 10.1111/pai.12365 9. Moody, M.W., Roberts, K.J., Huner, J.V. Phytogeny of commercially important seafood and description of the seafood industry (1993) Clinical Reviews in Allergy, 11 (2), pp. 159-181. Cited 12 times. doi: 10.1007/BF02914469 10. Yadzir, Z.H.M., Misnan, R., Abdullah, N., Bakhtiar, F., Arip, M., Murad, S. Identification of the major allergen of Macrobrachium rosenbergii (giant freshwater prawn) (2012) Asian Pacific Journal of Tropical Biomedicine, 2 (1), pp. 50-54. Cited 26 times. http://www.journals.elsevier.com/asian-pacific-journal-of-tropical-biomedicine/ doi: 10.1016/S2221-1691(11)60189-5 11. Thomison, J.B. Primum non nocere(1987) Southern Medical Journal, 80 (2), pp. 149-150. doi: 10.1097/00007611-198702000-00001 12. Smith, W. Skin Prick Testing for the Diagnosis of Allergic Disease (2013) Australasian Society of Clinical Immunology and Allergy, 39. Cited 2 times. 13. Zhang, Y., Matsuo, H., Morita, E. Cross-reactivity among shrimp, crab and scallops in a patient with a seafood allergy (2006) Journal of Dermatology, 33 (3), pp. 174-177. Cited 35 times. doi: 10.1111/j.1346-8138.2006.00040.x


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