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Type :thesis
Subject :QA76 Computer software
Main Author :Muhammad Yunus Hamid
Title :The development of optical stiffness calibration software base on equipartition theorem, boltzman statistics and power spectrum density
Place of Production :Tanjong Malim
Publisher :Fakulti Sains dan Matematik
Year of Publication :2018
Corporate Name :Universiti Pendidikan Sultan Idris
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Abstract : Universiti Pendidikan Sultan Idris
This  study  aimed  to  develop  an  optical  stiffness  calibration  system  using  three methods. The used methods were Boltzmann statistics (BS), equipartition theorem (ET) and power  spectrum density (PSD) analysis. This study consisted of two phases; system development phase and  testing phase. System development phase involved hardware and software components. Testing phase  involved actual experimental optical stiffness calibration. The finding of the study was a  calibration system consisting hardware and software parts. The hardware part was an optical  tweezers that was equipped with quadrant photodiode (QPD) and piezostage. The software part was a  custom made software which was constructed on LabVIEW 2012 platform, namely OSCal. This study  showed the conversion factor (used in ET and BS methods) and optical stiffness depends on trapping  laser power. In conclusion, this study successfully developed the aimed system with comparable  optical stiffness between each method and other reference studies. The implication of this study is  that the time spent for calibration for optical tweezers procedure can be shortened so that other researcher can focus more on the intended applications of the optical tweezers.  

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