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Type :thesis
Subject :RM Therapeutics. Pharmacology
Main Author :Nik Rabi'atul Adawiyah Nik Zulkifeli
Title :The effect of election beam irradiation on the quality of hydrolysed collagen and cell viability
Place of Production :Tanjong Malim
Publisher :Fakulti Sains dan Matematik
Year of Publication :2018
Corporate Name :Universiti Pendidikan Sultan Idris
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Abstract : Universiti Pendidikan Sultan Idris
The purpose of this study was to evaluate the effect of electron beam irradiation at different dosages towards the quality of hydrolysed collagen samples from Oreochromis mossambicus’s  scales. Hydrolysed collagen samples powder were irradiated using electron beam at doses of 5, 10,  15 and 20 kGy. After being irradiated, samples were individually vacuum-packaged and stored at 4°C  for four weeks. Then, the total number of coliform, yeast and mould, pH, macronutrients of samples,  and molecular weight of the samples’ protein were investigated. This study also determined the  effect of hydrolysed collagen samples at different concentrations towards the viability and  proliferation of skin fibroblast primary cell. The results showed that total number of coliform,  yeast and mould decreased with the increment of irradiation dosage, but, no coliform, yeast and  mould growth in 15 and 20 kGy samples. Meanwhile, the D10 value of coliform is 14.43 kGy whilst  yeast and mould is 13.76 kGy. The samples’ pH values and macronutrients evaluation did not show any significant  differences between non-irradiated and irradiated samples. Sodium dodecyl sulphate polyacrylamide  gel electrophoresis (SDS-PAGE) separated two bands of protein between 17.75 to 23.5 kDa. It also  showed that electron beam irradiation dosage did not cause any substantial change of  electrophoretic pattern on molecular weight of hydrolysed collagen. The concentration of hydrolysed  collagen between 4 mg/mL to 10 mg/mL has increased the viability and proliferation of normal skin  fibroblast primary cell. As a conclusion, the results suggest that electron beam irradiation is useful in improving microbial safety without impairing the quality of hydrolysed  collagen. The implication of this study is hydrolysed collagen from O.mossambicus’s scales can be  used to increase the number and proliferation of skin fibroblast cell and would be essential for any skin care and pharmaceutical use.  

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