|
UPSI Digital Repository (UDRep)
|
|
| ||||||||||||||||||||
| Abstract : Universiti Pendidikan Sultan Idris |
| Fungi are eukaryotic organisms that live as saprophytes, parasites or symbionts in their plant or animal hosts. Despite extensive research on the involvement of fungal allergens in the pathophysiology of allergic diseases, studies on fungi as a prominent source of allergens are still lacking in basic research and clinical practice. Hence, this study was conducted to identify ten common airborne fungal species as a preliminary work prior to conducting a sensitisation study on common fungal allergens. Fifty-four dust samples were collected from offices, laboratories lecturers’ rooms that are situated within three blocks of the Sultan Azlan Shah Campus, UPSI, Tanjong Malim, Perak, Malaysia by using a vacuum cleaner. The sieved dust was cultured with potato dextrose agar media, incubated at room temperature to propagate pure cultures and sent to the Malaysian Agricultural Research and Development Institute for identification viapolymerase chain reaction. The most common species found in the premises were Penicilliumsimplicissimum (94%), Aspergillus aculeatus (85%), Rhodosporidiobolusruineniae (74%), Ceriporialacerata (92%), A.caliodustus (57%), Syncephalastrumsp. (62%), Aspergillus sp. (72%), A. fumigatus (77%), Fusarium sp. (77%) and P.canescens (83%). A number of species identified in this study do not trigger fungal allergies. Therefore, further studies must be conducted to confirm their potential as fungi allergens. Due to high frequency of Penicillium and Aspergillus, The three-block research area in this study could be categorised as sick building and mycoremediation was recommended to minimise mould growth |
| References |
[1] Abdulmumin, U.A., Manga, S.B., Shinkafi, S.A., Rabah, A.B., Gambo, A., & Odeh, P. A. (2014). Annals of Biological Sciences Fungi associated with carpets and floor dust samples as an indicator for indoor air quality. Department of Microbiology, Usmanu Danfodio University, P.M. B 2346, Sokoto State-Nigeria Department of Microbiology, Bayero U, 2(4), 16–19. [2] Luongo, J. C., Barberán, A., Hacker?Cary, R., Morgan, E. E., Miller, S. L., & Fierer, N. (2017). Microbial analyses of airborne dust collected from dormitory rooms predict the sex of occupants. Indoor air, 27(2), 338-344. [3] Prussin, A. J., Garcia, E. B., & Marr, L. C. (2015). Total concentrations of virus and bacteria in indoor and outdoor air. Environmental Science and Technology Letters, 2(4), 84–88. [4] Amann, R. I., Ludwig, W., & Schleifer, K. H. (1995). Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiological Reviews, 59(1), 143–169. [5] Whitley, B. E., & Kite, M. E. (2018). Principles of research in behavioral science. Routledge. [6] Dannemiller, K. C., Weschler, C. J., & Peccia, J. (2017). Fungal and bacterial growth in floor dust at elevated relative humidity levels. Indoor Air, 27(2), 354-363. [7] Barberán, A., Ladau, J., Leff, J. W., Pollard, K. S., Menninger, H. L., Dunn, R. R., & Fierer, N. (2015). Continental-scale distributions of dust-associated bacteria and fungi. Proceedings of the National Academy of Sciences, 112(18), 5756-5761. [8] Ghiasian, S. A., Maghsood, A. H., & Aghamirian, M. R. (2016). Aeromycological analysis of allergenic airborne fungi in Qazvin, Iran. Current medical mycology, 2(3), 5–9. [9] ?ukiewicz-Sobczak, W. A. (2013). The role of fungi in allergic diseases. Advances in Dermatology and Allergology/Post?py Dermatologii I Alergologii, 30(1), 42-45. [10] Fukutomi, Y., & Taniguchi, M. (2015). Sensitization to fungal allergens: resolved and unresolved issues. Allergology International, 64(4), 321-331. [11] Twaroch, T. E., Curin, M., Valenta, R., & Swoboda, I. (2015). Mold allergens in respiratory allergy: from structure to therapy. Allergy, asthma & immunology research, 7(3), 205-220. [12] Macher, J.M. (2001). Review of methods to collect settled dust and isolate culturable microorganisms. Indoor Air, 11(2), 99–110. [13] WHO/IUIS Allergen Nomenclature Sub-Committee database 2019. [14] Savill, J., & Fadok, V. (2002). Dustborne fungi in large office buildings. Mycopathologia, 154(2), 93–106. [15] Abarca, M.L. (2000). Taxonomy and identification of the species involved in nosocomial aspergillosis. Revista iberoamericana de micologia, 17(3), S79—84. [16] Neofytos, D., Horn, D., Anaissie, E., Steinbach, W., Olyaei, A., Fishman, J., & Marr, K. (2009). Epidemiology and outcome of invasive fungal infection in adult hematopoietic stem cell transplant recipients: analysis of Multicenter Prospective Antifungal Therapy (PATH) Alliance registry. Clinical Infectious Diseases, 48(3), 265-273. [17] Kubak, B. M., & Huprikar, S. S. (2009). Emerging & rare fungal infections in solid organ transplant recipients. American Journal of Transplantation, 9(s4). [18] Hussin, N. H. M., Sann, L. M., Shamsudin, M. N., & Hashim, Z. (2011). Characterization of bacteria and fungi bioaerosol in the indoor air of selected primary schools in Malaysia. Indoor and Built Environment, 20(6), 607-617. [19] Rahman, W. A., Rosli, H., Baharuddin, S. N., & Salleh, B. (2012). Incidence and remediation of fungi in a sick building in Malaysia: a case study. Aerobiologia, 28(2), 275-283. [20] Hock, O. G., Cheng, H. C., Fang, V. L. B., Yong, W. Y., & Shing, W. L. (2018). Isolation and identification of potential fungal species for spent engine lubrication oil remediation in Peninsular Malaysia. Remediation Journal, 28(3), 91-95. [21] Talley, S. M., Coley, P. D., & Kursar, T. A. (2002). The effects of weather on fungal abundance and richness among 25 communities in the Intermountain West. BMC ecology, 2(1), 7–10. [22] Nevalainen, A., Täubel, M., & Hyvärinen, A. (2015). Indoor fungi: companions and contaminants. Indoor air, 25(2), 125-156.
|
| This material may be protected under Copyright Act which governs the making of photocopies or reproductions of copyrighted materials. You may use the digitized material for private study, scholarship, or research. |