Inducement of agarwood resin using various chemical formulation of stem tissue (Aquilaria malaccensis)

Saripah Salbiah Syed Abdul Azziz

Abstract

Agarwood is described as fragrance wood that is usually derived from the trunk of genus Aquilaria which have been infected by a particular disease. The main focus of this research is to study the effectiveness of four chemical inoculants applied into 6 year old karas tree (A. malaccensis) plantations in Slim River, Perak, Malaysia using dripping techniques. The inoculants used were labelled as A, B, C and D. Commercial inoculant, labelled as K, was used for comparison purposes and control labelled as N. After eight months of inoculation, the karas tree were cut down and discoloured tissue samples were collected. Solvent extraction methods using dichloromethane (DCM) was carried out to obtain the resin crude extract from the plant tissue. Resin crude extract were then analysed using gas chromatography-mass spectrometry (GC-MS). The results showed that all chemicals successfully stimulated agarwood production. Inoculant A produced the highest crude extract recovery (8.63%) compared to inoculants B (7.45%), D (6.55%), C (5.95%), K (1.89%) and N (0.49%). GC-MS analysis revealed that aromatic compounds, sesquiterpenes, monoterpenes, sterols, alkanes and fatty acid methyl ester were identified from DCM extract. As an outcome, inoculant A is the best to be used as stimulant for agarwood due to product highest yield. This finding will hopefully contribute to new types of inoculants production in agarwood industries in Malaysia.

References

1. Suharti S, Santosa E Turjama (2011) Feasibility Study of Business in Agarwood Inoculation and Different Stem Diameters and Inoculation Periods. J. For Res, 8(5): 114-129.

2. Barden A, Anak NA, Mulliken T, Song M (2000) Heart of the Matter: Agarwooduse and trade and CITES implementation for Aquilaria malaccensis, 1-52.

3. Napis S, Salleh K M, Itam K, Latiff A (2001) Biodiversity Databases for Malaysian Flora and Fauna: an Update. In Proceesings of IWS 2001 (Internet Workshop 2001) 21-23 6.

4. Zachary S Rogers (2009) A Revision of Malagasy Gnidia (Thymelaeaceae, Thymelaeoideae). Missouri Botanical Garden, 96(2): 324-369.

5. Lok E (2016) Growth And Management of Aquilaria malaccensis For Agarwood. International Journal of Agriculture, Forestry and Plantation, 3 55-60.

6. Lim TK, Norainie AA (2010) Wood for tress: A review of the agarwood (gaharu) trade in Malaysia. Traffic Southeast Asia.

7. Yahya A, Husin HR, Atan HM, Halip NLM, Hashim N, Hamid Z, Saleh SB & Bakar FA (2011) Industri Gaharu Malaysia: Cabaran dan Prospek.(2nd ed.). Lembaga Perindustrian Kayu Malaysia.

8. Jayachandran K, Sekar I, Parthiban KT, Amirtham D, Suresh KK (2014) Analysis of different grades of Agarwood (Aquilaria malaccensis Lamk.) oil through GC-MS. Indian J. Nat. Prod. Resource, 5(1): 44-47.

9. Radhuan Hussain (2014) Harga Gaharu RM130,000 /kg. Utusan Malaysia. Retrieved from https:// www. utusan. com.my/berita/nasional/harga-gaharurm130-000-kg-1.24374.

10. Yusof S, Nizam S, Mansor R, Wei P & Mazila AN (2018) Gas Chromatography Analysis of Artificially Inoculated Agarwood Compounds Related to High Quality Agarwood from Malaysia Plantation. Chemistry of Advanced Materials, 3(3): 60-66.

11. Rasool S, Mohamed R (2016) Understanding Agarwood Formation and Its Challenges. In R. Mohamed (Ed.), Agarwood: Science Behind the Fragrance (pp. 39-56) Singapore: Springer Singapore. https://doi.org/10.1007/978-981-10-08337_3.

12. Liu YY, Chen H, Yang Y, Zhang ZL, Wei JH, Meng H, Chen H (2013) Whole- Tree agarwood-inducing Technique: An efficient novel technique for producing high-quality agarwood in cultivated Aquilaria sinensis trees. Molecules, 18 (3): 3086-3106. https://doi.org/10.3390/molecules18033086.

13. Zhang XL, Liu YY, Wei JH, Yang YY, Zhang Z, Huang JQ, Liu YJ (2012) Production of high-quality agarwood in Aquilaria sinensis trees via whole-tree agarwood-induction technology. Chin. Chem. Lett., 23.

14. Okudera Y, Ito M (2009) Production of agarwood fragrant constituents in Aquilaria calli and cell suspension cultures. Plant Biotechnology, 26(3): 307-315.

15. Chen H, Yang YY, Xue J, Wei JH, Zhang ZL, & Chen H (2011) Comparison of Compositions and Antimicrobial Activities of Essential Oils from Chemically Stimulated Agarwood, Wild Agarwood and Healthy Aquilaria sinensis (Lour.) Gilg Trees. Molecules, 16(6): 4884. https:// doi.org/10.3390/molecules16064884.

16. Chua LSL (2008) Agarwood (Aquilaria malaccensis) in Malaysia. NDF Workshop Case Studies 3.

17. Zhang ZL, Wei JH, Han X, Liang L, YangYY, Meng H, Gao Z (2014) The sesquiterpene biosynthesis and vesselocclusion formation in stems of Aquilaria sinensi (lour.) gilg trees induced by wounding treatments without variation of microbial communities. International Journal of Molecular Sciences, 15(12):23589-23603. https:// doi. org/ 10. 3390 /ijms151223589.

18. Nor Azah MA, Chang YS, Mailina J, Abu Said A, Abd Majid J, Saidatul Husni S, Nik Yasmin Y (2008) Comparison of Chemical Profiles of Selected Gaharu Oils From Peninsular Malaysia. The Malaysian Journal of Analytical Sciences.

19. Tajuddin SN, Yusof MM (2010) Chemical composition of volatile oils of Aquilaria malaccensis (Thymelaeaceae) from Malaysia. Natural Products Communication.

20. Tamuli P, Boruah P, Samanta R (2004) Biochemical changes in agarwood tree (Aquilaria malaccensis Lamk.) during pathogenesis. Journal of Spices and Aromatic Crops, 13(2): 87-91.