Interactions of flavone and steroid from A. subintegra as potential inhibitors for porcine pancreatic lipase

Yuhanis Mhd Bakri

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Type :	article
Subject :	Q Science (General)
Main Author :	Yuhanis Mhd Bakri
Additional Authors :	Wong, Chee Fah Fauziah Abdullah Saripah Salbiah Syed Abdul Azziz Mastura Ibrahim
Title :	Interactions of flavone and steroid from A. subintegra as potential inhibitors for porcine pancreatic lipase

Place of Production :	Tanjong Malim
Publisher :	Fakulti Sains dan Matematik
Year of Publication :	2019
Corporate Name :	Universiti Pendidikan Sultan Idris
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Abstract : Universiti Pendidikan Sultan Idris

Background: Obesity is one serious health condition that contributes to various chronic diseases. The inhibition of pancreatic lipase is a promising treatment for obesity. Objective: The present study was designed to investigate anti-porcine pancreatic lipase effect of isolated compounds from Aquilaria subintegra and its mechanism. Method: Compounds were isolated with serial column chromatography and their structure were identified using spectroscopic methods. Isolated compounds were tested for anti-lipase potential activity using colorimetric assay. The prediction of energy binding between isolated compounds and enzyme was described using YASARA software. Results: Four compounds were successfully isolated from the bark of A. subintegra, namely, 5-hydroxy-7,4’-dimethoxyflavone, luteolin-7,3’,4’-trimethyl ether, 5,3’-dihydroxy-7,4’-dimethoxyflavone and β-sitosterol. The results indicated that all compounds displayed promising pancreatic lipase inhibitory activity ranging between of 6% to 53% inhibition. Compound 5-hydroxy-7,4’-dimethoxyflavone was a competitive inhibitor and decreases the enzyme catalysis. Meanwhile, βsitosterol was a non- competitive inhibitor since the latter was bind allosterically toward enzyme. Conclusion: This finding is significant for further investigation of bioactive compounds from A. subintegra on animal study

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