UPSI Digital Repository (UDRep)
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UPSI Digital Repository (UDRep)
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| Abstract : Universiti Pendidikan Sultan Idris |
| Objective: To investigate the effects of Aquilaria subintegra leaf aqueous extracts on the aphrodisiac properties including sexual behaviour, testosterone level, percentage of pregnancy, number of offspring and male to female ratio of offspring in ICR mice.
Methods: In this experiment, each male cohabitated with one female in a polysulfone cage. 30 ICR male mice were divided into 6 groups that received normal saline (the control group), 50 mg/kg, 100 mg/kg, 200 mg/kg, 500 mg/kg, and 1 000 mg/kg body weight of Aquilaria subintegra leaf aqueous extracts orally for 21 days consecutively. Sexual behavior, percentage of pregnancy, number of offspring and male to female ratio of offspring in ICR mice were measured according to the established methods. Testosterone level was measured by using enzyme-linked immunosorbent assay.
Results: Mice that received Aquilaria subintegra leaf aqueous extracts at 50 mg/kg body weight (day 0) had significantly higher mount frequency as compared to the control group; groups treated with 100, 500, 1 000 mg/kg body weight extracts produced a greater number of offsprings when compared to the control group. All aphrodisiac parameters were similar between the treatment groups and the control group, indicating that Aquilaria subintegra leaf aqueous extract did not significantly alter the aphrodisiac parameters.
Conclusions: Aquilaria subintegra leaf aqueous extracts have no effect on the aphrodisiac properties, but could increase the breeding rate in mice.
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| References |
[1] A’attiyyah AA, Wan Afiqah Syahirah WG, Nor Azah MA, Kannan TP, Suharni M, Ahmad A. Phytochemical properties and traditional uses of selected medicinal plants in Malaysia: A review. J Biomed Clin Sci 2017; 2(2): 14-25. [2] Saw LG, Chua LSL, Suhaida M, Yong WSY, Hamidah M. Conservation of some rare and endangered plants from Peninsular Malaysia. Kew Bulletin 2010; 65(4): 681-689. [3] Ong HC, Rosnaini MZ, Pozi Milow. Traditional knowledge of medicinal plants among the Malay villagers in Kampung Mak Kemas, Terengganu, Malaysia. Stud Ethno-Med 2011; 5(3): 175-185. [4] Sharma P, Bhardwaj P, Arif T, Khan I, Singh R. Pharmacology, phytochemistry and safety of aphrodisiac medicinal plants: A review. J Pharmacol Toxicol Stud 2014; 2(3): 1-18. [5] Redzuan Nul Hakim AR, Muhammad Lokman MI, Afzan MY, Azantee Yazmie AW, Hussin M, Roszaman R. Animal studies on male fertility enhancing properties of plants in Malaysia: A review of the past 16 years. J Biotechnol Strateg Health Res 2017; 1(1): 17-23. [6] Mathur M. Herbal aphrodisiac their need, biology and status: Global and regional scenario. J Nat Prod 2012; 5: 131-146. [7] Kotta S, Ansari SH, Ali J. Exploring scientifically proven herbal aphrodisiacs. Pharmacogn Rev 2013; 7(13): 1-10. [8] Norhazlina AW, Norfilza MM, Wan Nurul Heriza AH, Das S. The effect of Eurycoma longifolia Jack on spermatogenesis in estrogen treated rats. Clinics 2010; 65(1): 93-98. [9] Nor-Raidah R, Mahanem MN. Enhancement of fertility and libido in male Sprague Dawley rats following the administration of aqueous extract of Lunasia amara. Malays Appl Biol 2015; 44(1): 125-131. [10]Muhd Hanis MI, Siti Balkis B, Mohamad O, Jamaludin M. Protective role of Hibiscus sabdariffa calyx extract against streptozotocin induced sperm damage in diabetic rats. EXCLI J 2012; 11: 659-669. [11]Bahrani H, Mohamad J, Paydar MJ, Rothan HA. Isolation and characterization of acetylcholinesterase inhibitors from Aquilaria subintegra for the treatment of Alzheimer’s disease (AD). Curr Alzheimer Res 2014; 11(2): 206-214. [12]Hara H, Ise Y, Morimoto N, Shimazawa M, Ichihashi K, Ohyama M, et al. Laxative effect of agarwood leaves and its mechanism. Biosci Biotechnol Biochem 2008; 72(2): 335-345. [13]Rahman H, Eswaraiah MC, Dutta AM. Anti-arthritic activity of leaves and oil of Aquilaria agallocha. Saudi J Life Sci 2016; 1(1): 34-43. [14]Nik Noor Asma NW, Nor Adila MO, Noorhuda AI, Saiful Nizam T. In vitro antioxidant activity and phytochemical screening of Aquilaria malaccensis leaf extracts. J Chem Pharm Res 2014; 6(12): 688-693. [15]Ibrahim M, Syed Abdul Azziz SS, Wong CF, Wan Mohamad Din WNI, Wan Mahamod WR, Bakri YM, et al. Evaluation of anti-lipase activity of leaf and bark extracts from Aquilaria subintegra and A. malaccensis. Mamar Pharm J 2018; 22(1): 91-95. [16]Yang L, Qiao L, Xie D, Yuan Y, Chen N, Dai J, et al. 2-(2-phenylethyl) chromones from Chinese eaglewood. Phytochemistry 2012; 76: 92-97. [17]Chen HQ, Wei JH, Yang JS, Zhang Z, Yang Y, Gao ZH, et al. Chemical constituents of agarwood originating from the endemic genus Aquilaria plants. Chem Biodivers 2012; 9(2): 236-250. [18]Fatmawati, Hidayat R. Anti-cancer activity of Aquilaria malaccensis leaves on human. Eur J Pharm Med Res 2016; 3(1): 46-49. [19]Alam J, Mujahid M, Rahman M, Akhtar J, Khalid M, Jahan Y, et al. An insight of pharmacognostic study and phytopharmacology of Aquilaria agallocha. J Appl Pharm Sci 2015; 5(8): 173-181. [20]Yakubu MT, Akanji MA, Oladiji AT. Aphrodisiac potentials of the aqueous extract of Fadogia agrestis (Schweinf. Ex Hiern) stem in male albino rats. Asian J Androl 2005; 7(4): 399-404. [21]Zamblé A, Martin-Nizard F, Sahpaz S, Reynaert ML, Staels B, Bordet R, et al. Effects of Microdesmis keayana alkaloids on vascular parameters of erectile dysfunction. Phytother Res 2009; 23(6): 892-895. [22]Silva CV, Borges FM, Velozo ES. Phytochemistry of some Brazilian plants with aphrodisiac activity. Brazil: Federal University of Bahia; 2012. [23]Ghan SY, Chin JH, Thoo YY, Yim HS, Ho CW. Acute oral toxicity study of Aquilaria crassna and?-tocopherol in mice. Int J Pharm Sci Res 2016;7(4): 1456-1461. [24]Erhirhie EO, Ekene NE, Ajaghaku DL. Guidelines on dosage calculation and stock solution preparation in experimental animals’ studies. J Nat Sci Res 2014; 4(18): 100-106. [25]Zade V, Dabhadkar D, Thakare V, Pare S. Evaluation of potential aphrodisiac activity of Moringa oleifera seed in male albino rats. Int J Pharm Pharm Sci 2013; 5(4): 683-689. [26]Mokhtari M, Zanboori M. The effects of lead acetate on sexual behavior and the level of testosterone in adult male rats. Int J Fertil Steril 2011;5(1): 13-20. [27]Palaniyappan V, Kothandam H, Vasu S, Moses P, Sundaram S. Evaluation of phytoconstituents and aphrodisiac activity of the fruits of Durio zibenthinus Linn. J Pharm Res 2009; 2(9): 1493-1495. [28]Low BS, Das PK, Chan KL. Standardized quassinoid-rich Eurycoma longifolia extract improved spermatogenesis and fertility in male rats via the hypothalamic-pituitary-gonadal axis. J Ethnopharmacol 2013; 145(3):706-714. [29]Balamurugan G, Muralidharan P, Polapala S. Aphrodisiac activity and curative effects of Pedalium murex (L.) against ethanol-induced infertility in male rats. Turk J Biol 2010; 34: 153-163. [30]Subramoniam A, Madhavachandran V, Ravi K, Anuja VS. Aphrodisiac property of the elephant creeper Argyreia nervosa. J Endocrinol Reprod 2007; 11(2): 82-85. [31]Dulay RMR, Apolinar AAC, Tiniola RC, Kalaw SP, Reyes RG. Aphrodisiac and diuretic activity of Philippine wild higher Basidiomycetes, Ganoderma lucidum (W. Curt.: Fr.) P. Karst., extract in male mice (Mus musculus). Adv Environ Biol 2016; 10(1): 144-149. [32]Dasofunjo K, Asuk AA, Ezugwu HC, Nwodo OFC, Olatunji TL. Aphrodisiac effect of ethanol extract of Piliostigma thonningii leaf on male albino Wistar rats. J Appl Pharm Sci 2013; 3(10): 130-135. [33]Wawata AU, Dikko AAU, Olorunshola KV, Wawata MU, Attahir A, Ahmed MK. The effect of aqueous methanolic stem bark extracts of Acacia polyacantha on sexual behaviour, serum testosterone levels in male Wistar rats. Asian J Med Sci 2010; 2(3): 138-140. [34]Fouche G, Afolayan AJ, Wintola OA, Khorombi TE, Senabe J. Effect of the aqueous extract of the aerial parts of Monsonia angustifolia E. Mey. Ex A. Rich., on the sexual behaviour of male Wistar rats. BMC Complement Altern Med 2015; 15(1): 343-353. [35]Nchegang B, Mezui C, Longo F, Nkwengoua ZE, Amang AP, Tan PV.Effects of the aqueous extract of Eremomastax speciosa (Acanthaceae) on sexual behavior in normal male rats. Biomed Res Int 2016; 2016: 1-10. [36]Che Musa NH, Mohd Zain HH, Ibrahim H. Aphrodisiac properties of Aquilaria malaccensis leaves aqueous extract in ICR mice. J Res Pharm 2019; 23(1): 130-140. [37]Yakubu MT, Awotunde OS, Ajiboye TO, Oladiji AT, Akanji MA. Prosexual effects of aqueous extracts of Massularia acuminata root in male Wistar rats. Andrology 2011; 43(5): 334-340. [38]Patel DK, Kumar R, Laloo D, Sairam K, Hemalatha S. Aphrodisiac activity of ethanolic extract of Pedalium murex Linn fruit. Asian Pac J Trop Biomed 2012; 2(3): S1568-S1571. [39]Oto EO. An evaluation of the aphrodisiac and fertility enhancing effect of the methanolic root extract of Cissus populnea Guill. and Perr. (Vitaceae.) Doctoral dissertation. Ahmadu Bello University; 2015. [40]Yakubu MT, Quadri AL. Garcinia kola seeds: Is the aqueous extract a true aphrodisiac in male Wistar rats? Afr J Tradit Complement Altern Med 2012; 9(4): 530-535. [41]Ugwah-Oguejiofor CJ, Bello SO, Okolo RU, Etuk EU, Ugwah MO, Igbokwe VU. Ficus platyphylla promotes fertility in female Rattus norvegicus Wistar strain: A preliminary study. Reprod Biol Endocrinol 2011; 9(1): 145-151. [42]Saxena M, Saxena J, Nema R, Singh D, Gupta A. Phytochemistry of medicinal plants. J Pharmacogn Phytochem 2013; 1(6): 168-182. [43]Najafi S. Pyhtochemical screening and antibacterial activity of leaf extract of Ziziphus mauritiana Lam. Int J Sci Basic Appl Res 2013; 4(11):3274-3276. [44]Sheweita SA, Tilmisany AM, Al-Sawaf H. Mechanisms of male infertility: Role of antioxidants. Curr Drug Metab 2005; 6(5): 495-501. [45]Hamad AWR, Al-Daghistani HI, Shquirat WD, Abdel-Dayem M, AlSwaif M. Sodium, potassium, calcium and copper levels in seminal plasma are associated with sperm quality in fertile and infertile men. Biochem Pharmacol 2014; 3(4): 1-7.
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