Antibacterial activity of phytochemicals from garcinia parvifolia miq. and garcinia hombroniana pierre

Noor Amira Muhammad

Abstract

Phytochemical studies on the stem bark of Garcinia parvifolia and pericarp of Garcinia hombroniana have been investigated. The crude extracts were purified using chromatographic techniques to obtain pure compounds named as rubraxanthone (1), parvixanthone G (2) cowanin (3), garcihombronane B (4), garcihombronane C (5) and stigmasterol (6). The structures of all compounds were characterized by spectroscopic methods. The extracts and phytochemicals were tested for their antibacterial activity using disc diffusion method, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Acetone extract of the pericarp of G. hombroniana showed the most significant antibacterial activity with MIC and MBC values of 225 μg/mL, meanwhile compound (3) exhibited the most significant antibacterial activity compared to other xanthones with MBC value of 225 μg/mL.

References

Aligians, N., Kalpoutzakis, E., Mitaku, S., Chinou, I.B. (2001). Composition and antimicrobial activity of the essential oil from Origanum Species. Journal of Agricultural and Food Chemistry, 49. 4168-4170.

Dharmaratne, H.R.W., Sakagami, Y., Piyasena, K.G.P., Thevanesam, V. (2013). Antibacterial activity of xanthones from Garcinia mangostana (L.) and their structure-activity relationship studies. Natural Product Research, 27(10), 938-41.

Ee, G.C.L., Ng, S.H., Goh, J.K., Sukari, M.A., Rahmania, M. (2009). Chemical constituents of Garcinia parvifolia (Guttiferae). Malaysian Journal of Science, 28 (1), 105-110.

Iinuma, M., Tosa, H., Tanaka, T., Asai, F., Kobayashi, Y., Shimano, R., Miyauchi, K. (1996). Antibacterial activity of xanthones from guttiferaeous plants against methicillin-resistant Staphylococcus aureus. Journal of Pharmacy and Pharmacology, 48(8), 861-865.

John, K.J., Senthil Kumar, R., Suresh, C.P., George, J.K., Abraham, Z. (2008). Occurrence, distribution and economic potential of seashore mangosteen (Garcinia hombroniana Pierre) in India. Genetic Resources and Crop Evolution, 55, 183-186.

Kardono, L.B.S., Hanafi, M., Sherley, G., Kosela, S., Harrison, L.J. (2006). Bioactive constituents of Garcinia porrecta and G. parvifolia Grown in Indonesia. Pakistan Journal of Biological Sciences, 9(3), 483-486.

Mbaveng, A.T., Ngameni, B., Kuete, V., Simo, I.K., Ambassa, P., Roy, R., Bezabih, M., Etoa, F.X., Ngadjui, B.T., Abegaz, B.M., Meyer, J.J., Lall, N., Beng, V.P. (2008). Antimicrobial activity of the crude extracts and five flavanoids from the twigs of Dorstenia barteri (Moraceae). Journal of Ethnopharmacology, 116. 489-489.

Mori, A., Nishino, C., Enoki, N., Tawata, S. (1987). Antibacterial activity and mode of plant flavonoids against Proteus vulgaris and Staphylococcus aureus (1987). Phytochemistry, 26. 223-224.

Najmuldeen, I.A., Hadi, A.H., Mohamad, K., Awang, K., Nasab, M.F., Ketuly, K.A., Mukhtar, M.R., Morita, H. (2011). Steroids from Chisocheton Tomentosus. Malaysian Journal of Science, 30(2), 144-153.

Nguyen, H.D., Trinh, B.T.D., Tran, Q.N., Pham, H.D., Hansen, P.E., Duus, F., Connolly, J.D., Nguyen, L.D. (2011). Friedolanostane, friedocycloartane and benzophenone constituents of the bark and leaves of Garcinia benthami. Phytochemistry, 72, 290-295.

Niu, S., Li, Z., Ji, F., Liu, G., Zhao, N., Liu, X., Jing, Y., Hua, H. (2012). Xanthones from the stem bark of Garcinia bracteata with growth inhibitory effects against HL-60 cells. Phytochemistry, 77, 280-286.

Pattalung, P., Thongtheeraparp, W., Wiriyachitra, P.C., Taylor, W. (1994). Xanthones of Garcinia cowa. Planta Medica, 60, 365-368.

Rukachaisirikul, V., Saelim, S., Karnsomchoke, P., Phongpaichit, S. (2005). Friedolanostanes and lanostanes from the leaves of Garcinia hombroniana. Journal of Natural Products, 68, 1222-1225.

Rukachaisirikul, V., Trisuwan, K., Sukpondma, Y., Phongpaichit, S. (2008). A new benzoquinone derivative from the leaves of Garcinia parvifolia. Archives of Pharmacal Research, 31(1), 17-20.

Vieiraa, L.M., Kijjoaa, A., Silvad, A.M.S., Mondranondrae, I., Kengthonge, S., Galesa, L., Damasa, A. M., Herz, W. (2004). Lanostanes and friedolanostanes from the bark of Garcinia speciosa. Phytochemistry, 65, 393-398.

Xu, Y., Lai, Y., Imiyabir, Z., Goh, S. (2001). Xanthones from Garcinia parvifolia. Journal of Natural Products, 64, 1191-1195.