UPSI Digital Repository (UDRep)
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Abstract : Universiti Pendidikan Sultan Idris |
This study performed to investigate the chemical compounds, toxicology, in vitro and
in vivo antioxidant capacity of Malaysian Peperomia pellucida plant towards ICR mice.
The qualitative chemical compound test was performed and the antioxidant capacity of P.
pellucida plant extract was analyzed by 2,2-diphenyl-1-picrylhydrazyl (DPPH), β-carotene
bleaching and oxygen radical absorbance capacity (ORAC) assays. The in vivo sub-acute
toxicity study that used the best solvent from in vitro assay was done using ICR mice which were
randomly divided. The different doses of 50 mg/kg, 150 mg/kg, 200 mg/kg, 500 mg/kg and 1000 mg/kg
body weight of extract were administered daily for 21 days to the treated mice. The antioxidant
enzymes in liver and kidney of mice were investigated using standard assays. P. pellucida only
exhibited the presence of alkaloid, phenol and saponin. The ability to scavenge free radical in
DPPH assay showed that methanol extract of Selangor (ground) sample depicted the highest value of
1108 ± 5.568 mg/ml compared to the other samples. In β-carotene bleaching assay, the ethyl
acetate extract of Penang (ground) sample offered the highest percentage (86.111%) of
scavenging activity from free radical compared to the other samples. In ORAC assay,
methanol extract of Penang (ground) sample showed the highest value 397.691 μmol of Trolox
equivalent/μl. Findings showed that ethyl acetate and methanol extracts exhibited a good solvent
for antioxidant capacity of P. pellucida. However, in sub-acute toxicity study, ethyl
acetate and methanol extract of higher doses lead to mortality in mice. Furthermore, the
administration of ethyl acetate and methanol extract of P. pellucida did not affect the antioxidant
enzymes in liver and kidney of mice. In conclusion, the antioxidant capacity of P. pellucida can
only be seen by in vitro assay. Thus, P. pellucida could be a potential source of
antioxidant substance.
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References |
A'attiyyah, A. A., Wan Afiqah Syahirah WG., Nor Azah M. A., Kannan T. P., Suharni, M., & Ahmad, A. (2017). Phytochemical properties and traditional uses of selected medicinal plants in Malaysia: A review. Journal of Biomedical and Clinical Sciences, 2(2), 14-25.
Abas Hussin. (2001). Adverse effects of herbs and drug-herbal interactions. Malaysian Journal of Pharmacy, 1(2), 39-44.
Abdille, Md. H., Singh, R. P., Jayaprakasha, G. K., & Jena, B. S. (2005). Antioxidant activity of the extracts from Dillenia indica fruits. Food chemistry, 90, 891-896.
Abere, T. A., & Okpalaonyagu, S. O. (2015). Pharmacognostic evaluation and antisickling activity of the leaves of Peperomia pellucida (L.) HBK (Piperaceae). African Journal of Pharmacy and Pharmacology, 9(21), 561-566.
Abeysinghe Pushpa, D., & Weeraddana Chaminda, De S. (2011). Screening of petroleum ether, chloroform, ethyl acetate, ethanol and water extracts of medicinal plant, Avicennia marina for antibacterial activity against antibiotic resistant bacteria species, Staphylococcus and Proteus. Journal of Pharmaceutical and Biomedical Sciences, 11 (11),1-4.
Abourashed, E. A. (2013). Bioavailability of plant-derived antioxidants. Antioxidants, 2, 309-325.
Adeshina, G. O., Kunle, O. F., Onaolapo, J. A., Ehinmidu, J. O., & Odama, L. E. (2012). Phytochemical and antibacterial studies of the hexane extract of Alchornea cordifolia leaf. Phytochemicals as Nutraceuticals-Global Approaches to Their Role in Nutrition and Health (pp. 90). Nigeria.
Agrawal, M., Agrawal, Y., Itankar, P., Patil, A., Vyas, J., & Kelkar, A. (2012). Phytochemical and HPTLC studies of various extracts of Annona squamosa (Annonaceae). International Journal of PharmaTech Research, 4(1), 364-368.
Ahmed, S. M., Ahmed, S., Tasleem, F., ul Hasan, M. M., & Azhar, I. (2012). Acute systemic toxicity of four Mimosaceous plants leaves in mice. Journal of Pharmacy, 2(2), 291-295.
Ahmed, M. (2015). Acute toxicity (lethal dose 50 calculation) of herbal drug Somina in rats and mice. Pharmacology & Pharmacy, 6, 185-189.
Akinnibosun, H. A., Akinnibosun, F. I., & German, B. E. (2008). Antibacterial activity of aqueous and ethanolic leaf extract of Peperomia peluucida (L.) H.B & K. (Piperaceae) on three gram-negative bacteria isolates, Science World Journal, 3(4), 33-36.
Alam, M. N., Bristi, N. J., & Rafiquzzaman, Md. (2013). Review on in vivo and in vitro methods evaluation of antioxidant activity. Saudi Pharmaceutical Journal, 21, 143-152.
Ali, H. S., Caballero, C. E., Maunting, N. G., Patricio, M. J. S., & Reyes, L. D. D. (2014). The Effect of Peperomia pellucida (Shiny Bush) leaf crude extract on the body temperature of boiled milk-induced male white mice. Advancing Pharmacy Research, 1, 70-82.
Ali Khairullah Zahi, Hazilawati Hamzah, Mohd Rosly Shaari, Widodo, R. T., Johnny, L., Noordin, M. M., et al (2016). Evaluation of acute and sub-acute dermal toxicity studies of ethanolic leaf extract of Lawsonia Inermis in Rats. Journal of Advances in Biotechnology, 6(1), 835-847.
Ali, R., Ali, R., Jaimini, A., Nishad, D. K., Mittal, Q., Chaurasia, O. P., et al. (2012). Acute and sub-acute toxicity and efficacy studies of Hippophae rhamnoides based herbal antioxidant supplement. Indian Journal of Pharmacology, 44(4), 504-509.
Al-Malki, A. L. (2010). Antioxidant properties of thymol and butylated hydroxytoluene in carbon tetrachloride-induced mice liver injury. Journal of King Abdulaziz University: Sciences, 22(1), 239-248.
Alsarhan, A., Sultana, N., Al-Khatib, A., & Mohammed Rafiq Abdul Kadir (2014). Review on some Malaysian traditional medicinal plants with therapeutic properties. Journal of Basic & Applied Sciences, 10, 149-159.
Altemimi, A., Lakhssassi, N., Baharlouei, A., Watson, D. G., & Lightfoot, D. A. (2017). Phytochemicals: Extraction, isolation, and identification of bioactive compounds from plant extracts. Plants, 6, 1-23.
Amel, B., (2015). Screening of the antioxidant and hypotensive activities of some medicinal plants in relation to their polyphenolic contents. (Doctoral dissertation, University of Ferhat Abbas). Retrieved from https://www.univsetif. dz/Tdoctorat/2015/SNV/BOUAZIZ%20AMEL.PDF.
Amin, M., Jassal, M. M. S., & Tygi, S. V. (2013). Phytochemical screening and isolation of Eugenol from Syzygium aromaticum by gas chromatography. International Journal of Research in Phytochemistry and Pharmacology, 3(1), 74-77.
Amutha, M., Geetha, A., & Amutha, M. (2014). A pilot study on the determination of antioxidant potential and lethal dosage of hydro alcoholic fruit extract of Terminalia Chebula. International Journal of Pharmacy and Pharmaceutical Sciences, 6(6), 346-351.
Anbudhasan, P., Surendraraj, A., Karkuzhali, S. & Sathishkumaran P. (2014). Natural antioxidants and its benefits. International Journal of Food and Nutritional Sciences, 3(6), 225-232.
Anderson, G. E. (2011). Solvent extraction. Retrieved from http://lipidlibrary.aocs.org/OilsFats/content.cfm?ItemNumber=40337.
Aniagu, S. O., Nwinyi, F. C., Akumka, D. D., Ajoku, G. A., Dzarma, S., Izebe, K. S., et al (2005). Toxicity studies in rats fed nature cure bitters. African Journal of Biotechnology, 4(1), 72-78.
Anisuzzaman, A. S. M., Sugimoto, N., Sadik, G., & Gafur, M. A. (2001). Sub-acute toxicity study of 5-hydroxy-2 (hydroxymethyl) 4H-pyran-4-One, isolated from Aspergillus fumigatus. Pakistan Journal of Biological Sciences, 4(8), 1012- 1015.
Anokwuru, C. P., Anyasor, G. N., Ajibaye, O., Fakoya, O., & Okebugwu, P. (2011). Effect of extraction solvents on phenolic, flavonoid and antioxidant activities of three nigerian medicinal plants. Nature and Science, 9(7), 53-61.
Anthony, K. P. & Saleh, M. A. (2013). Free radical scavenging and antioxidant activities of silymarin components. Antioxidants, 2, 398-407.
Anwar, F., Qayyum, H. M. A., Hussain, A. I., & Iqbal, S. (2010). Antioxidant activity of 100% and 80% methanol extracts from barley seeds (Hordeum vulgare L.): Stabilization of sunflower oil. Grasas y aceites, 61(3), 237-243.
Apak, R., Gorinstein, S., Böhm, V., Schaich, K. M., Özyürek, M., & Güçlü, K. (2013). Methods of measurement and evaluation of natural antioxidant capacity/activity (IUPAC Technical Report). Pure and Applied Chemistry, 85(5), 957-998.
Aziba, P. I., Adedeji, A., Ekor, M., & Adeyemi, O. (2001). Analgesic activity of Peperomia pellucida aerial parts in mice. Fitoterapia, 72, 57-58.
Azizah Othman, Nor Juwariah Mukhtar, Nurul Syakirin Ismail & Chang, S. K. (2014). Phenolics, flavonoids content and antioxidant activities of 4 Malaysian herbal plants. International Food Research Journal, 21(2), 759-766.
Badarinath, A. V., Rao, K. M., Chetty, C. M. S., Ramkanth, S., Rajan, T. V. S., & Gnanaprakash, K. (2010). A review on in-vitro antioxidant methods: Comparisions, correlations and considerations. International Journal of PharmTech Research, 2(2), 1276-1285.
Badrulhadza Amzah (2016). Bab 8: Pengurusan penyakit dan perosak (pp 64). Retrieved from https://www.researchgate.net/publication/315696807_Pengurusan_Penyakit_da n_Perosak
Bagchi, K. & Puri, S. (1998). Free radicals and antioxidants in health and disease: A review. La Revue de Sante de la Mediterranee Orientale, 4(2), 350-360.
Bagewadi, Z. K., Siddanagouda, R. S., & Baligar, P. G. (2012). Phytochemical screening and evaluation of antimicrobial activity of Semecarpus anacardium nuts. International Journal of Pharmacology and Pharmaceutical Technology, 1(2), 68-74.
Bajpai, V. K., Majumder, R., & Park, J. G. (2016). Isolation and purification of plant secondary metabolites using column-chromatographic technique. Bangladesh Journal of Pharmacology, 11(4), 844-848.
Baki, M. A., Khan, A., Al-Bari, M. A. A., Mosaddik, A., Sadik, G., & Mondal, K. A. M. S. H. (2007). Sub-acute toxicological studies of pongamol isolated from Pongamia pinnata. Research Journal of Medicine and Medical Sciences, 2(2), 53-57.
Balahoro?lu, R., Dülger, H., Özbek, H., Bayram, ?., & ?ekero?lu, M. R. (2015). Protective effects of antioxidants on the experimental liver and kidney toxicity in mice. European Journal of General Medicine, 5(3), 157-164.
Barchan, A., Bakkali, M., Arakrak, A., Pagán, R., & Laglaoui, A. (2014). The effects of solvents polarity on the phenolic contents and antioxidant activity of three Mentha species extracts. International Journal of Current Microbiology and Applied Sciences, 3(11), 399-412.
Batugal, P. (2004). Inventory and documentation of medicinal plants in 14 Asia Pacific countries. In Batugal, P. A., Kanniah, J., Lee, Sy., & Oliver, J. T. (Eds.), Medicinal plants research in Asia: Volume I. The framework and project workplans (pp. 6). Selangor, Malaysia: International Plant Genetic Resources Institute.
Bello, I., Bakkouri, A. S., Tabana, Y. M., Al-Hindi, B., Al-Mansoub, M. A., Roziahanim Mahmud et al (2016). Acute and sub-acute toxicity evaluation of the methanolic extract of Alstonia scholaris stem bark. Medical Sciences, 4(4), 1-14.
Benhammou, N., Ghambaza, N., Benabdelkader, S., Atik-Bekkara, F., & Panovska, F. K. (2013). Phytochemicals and antioxidant properties of extracts from the root and stems of Anabasis articulata. International Food Research Journal, 20(5), 2057-2063.
Bernhof, A. (2010). A brief review on bioactive compounds in plants In Bernholf, A (Eds.), Bioactive Compounds in Plants-Benefits and Risks for Man and Animals (pp. 11). Norway: The Norwegian Academy of Science and Letters.
Bhardwaj, S., & Gupta, D. (2012). Study of acute, subacute and chronic toxicity test. International Journal of Advanced Research in Pharmaceutical & Bio Sciences, 2(2), 103-129.
Bhattacharya, S. (2015). Reactive oxygen species and cellular defense system. In Rani, V. & Yadav, U. C. S. (Eds.), Free radicals in human health and disease (pp. 21,27), India: Springer India.
Bishi, S. K., Patil, A., Hota, M. R., Dagla, M. C., & Kumar, N. (2014). Health benefiting natural antioxidants: Present and future perspectives. Popular Kheti, 2(2), 198-201.
Bradford, M. M. (1976). A rapid and sensitive method for quantitation of micrograms quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72, 248-254.
Brandsch, C., Schmidt, T., Behn, D., Weiße, K., Mueller, A. S., & Stangl, G. I. (2010). Glutathione deficiency down-regulates hepatic lipogenesis in rats. Lipids in Health and Disease, 9(50), 1-8.
Breitenbach, M. & Eckl, P. (2015). Introduction to oxidative stress in biomedical and biological research. Biomolecules, 5, 1169-1177.
Canda, B. D. (2012). Modulation of oxidative stress by rooibos (Aspalathus linearis) herbal tea, chinese green (Camellia sinensis) tea and commercial tea supplements using a rodent model (Doctoral dissertation, Cape Peninsula University of Technology). Retrieved from http://etd.cput.ac.za/bitstream/handle/20.500.11838/1506/BarThesis.pdf?sequen ce=1&isAllowed=y
Carlsen, M. H., Halvorsen, B. L., Holte, K., Bøhn, S. K., Dragland, S., Sampson, L., et al (2010). The total antioxidant content of more than 3100 foods, beverages, spices, herbs and supplements used worldwide. Nutrition journal, 9(3), 1-11.
Carson, T. L., Osweiler, G. D., & Thorne, P. S. (2002). Adverse Health Effects: Toxicology. Iowa Concentrated Animal Feeding Operations Air Quality Study (pp. 104)
Cearfoss, J. M. (2012). The induction of oxidative stress in the livers of mice following long-term exposure to the water chlorination by-products, dichloroacetate and trichloroacetate. (Doctoral dissertation, University of Toledo). Retrieved from https://pdfs.semanticscholar.org/af75/59efb17ac8054292d67afa297b8069e61e8 9.pdf.
Chanda, S. & Dave, R. (2009). In vitro models for antioxidant activity evaluation and some medicinal plants possessing antioxidant properties: An overview. African Journal of Microbiology Research, 3(13), 981-996.
Chanda, S. V. & Kaneria, M. J. (2012). Optimization of conditions for the extraction of antioxidants from leaves of Syzygium cumini L. using different solvents. Food Analytical Methods, 5, 332-338.
Chen, G., Wang, S., Huang, X., Hong, J., Du, L., Zhang, L., et al. (2015). Environmental factors affecting growth and development of Banlangen (Radix Isatidis) in China. African Journal of Plant Science, 9(11), 421-426.
Chia, R., Achilli, F., Festing, M. F. W., & Fisher, E. M. C (2005). The origins and uses of mouse outbred stocks. Nature genetics, 37(11), 1181-1186.
Chun, O. K., Floegel, A., Chung, S. J., Chung, C. E., Song, W. O., & Koo, S. I. (2009). Estimation of antioxidant intakes from diet and supplements in US adults. The Journal of Nutrition, 140(2), 317-324.
Cichoski, A. J., Rotta, R. B., Scheuermann, G., Cunha Junior, A., & Barin, J. S. (2012). Investigation of glutathione peroxidase activity in chicken meat under different experimental conditions. Food Science and Technology, 32(4), 661- 667.
County, K. (2014). Garden tips, soil: It’s more than just dirt! Retrieved from https://web.extension.illinois.edu/hkmw/downloads/65475.pdf
Cox, G. V. (1985). The use of animals in testing. Alternatives to Animal Use in Research, Testing, and Education (pp. 149, 153, 154, 155). United States of America. Chemical Manufacturers Association.
Cri?an, C. C., Cãlinescu, I., Zãlaru, C., & Moldovan, Z. (2013). Techniques for extracting polyphenols from Coreopsis tinctoria nutt. fruits. University Politehnica of Bucharest Scientific Bulletin, 75(4), 169-178.
Crouse, K. & Denny, G. (2015). Soil ph and fertilizers. Retrieved from https://extension.msstate.edu/sites/default/files/publications/informationsheets/ is0372_1.pdf
Dai, J. & Mumper, R. J. (2010). Plant phenolics: extraction, analysis and their antioxidant and anticancer properties. Molecules, 15, 7313-7352. Danesi, F. & Ferguson, L. R. (2017). Could pomegranate juice help in the control of inflammatory diseases?. Nutrients, 9, 1-23.
Das, K., Tiwari, R. K. S., & Shrivastava, D. K (2010). Techniques for evaluation of medicinal plant products as antimicrobial agents; Current methods and future trends. Journal of Medicinal Plants Research, 4(2), 104-111.
Dávalos, A., Bartolomé, B., Suberviola, J., & Gómez-Cordovés, C. (2003). ORACfluorescein as a model for evaluating antioxidant activity of wines. Polish Journal of Food and Nutrition Sciences, 12(53), 133-136.
da Silva, M. H. L., Zoghbi, M. D. G. B., Andrade, E. H. A., & Maia, J. G. S. (1999). The essential oils of Peperomia pellucida Kunth and P. circinnata Link var. circinnata. Flavour and Fragrance Journal, 14, 312-314.
Dayang Norafizan Awang Chee (2008). Phytochemical studies and biological activities of the methanol extract of Peperomia pellucida. (Bachelor dissertation, Universiti Malaysia Sarawak).
Decoteau, D. (1998). Plant physiology: Environmental factors and photosynthesis. In Greenhouse Glazing & Solar Radiation Transmission Workshop, Department of Horticulture, Pennsylvania State University, USA. Retrieved from http://citeseerx.ist.psu.edu/viewdoc/download;jsessionid=455C463C5368E7C5 7259710BD9B593B0?doi=10.1.1.499.2367&rep=rep1&type=pdf
de Fátima, A. B. M., Oliveira, R. L. B., Mendes, S. S., de Albuquerque S. P., Antoniolli, Â. R., Vilar, J. C., et al (2002). Seed germination, phenology, and antiedematogenic activity of Peperomia pellucida (L.) HBK. BMC pharmacology, 2(12), 1-8.
de Fátima, A. B., M., Dmitrieva, E. G., Franzotti, E. M., Antoniolli, A. R., Andrade, M. R., & Marchioro, M. (2004). Anti-inflammatory and analgesic activity of Peperomia pellucida (L.) HBK (Piperaceae). Journal of Ethnopharmacology, 91(2004), 215-218.
Diehl, K. H., Hull, R., Morton, D., Pfister, R., Rabemampianina, Y., Smith, D., et al (2001). A good practice guide to the administration of substances and removal of blood, including routes and volumes. Journal of Applied Toxicology, 21, 15- 23.
Donno, D., Beccaro, G. L., Mellano, M. G., Cerutti, A. K., & Bounous, G. (2013). Medicinal plants, chemical composition and quality: May blackcurrant buds and blackberry sprouts be a new polyphenol source for herbal preparations?. Journal of Applied Botany and Food Quality, 86, 79-89.
dos Santos, P. R. D., de Lima Moreira, D., Guimarães, E. F., & Kaplan, M. A. C. (2001). Essential oil analysis of 10 Piperaceae species from the Brazilian Atlantic forest. Phytochemistry, 58, 547-551.
Dutta, M., Biswas, U. K., Chakraborty, R., Banerjee, P., Kumar, A., & Raychaudhuri, U. (2014). Enhanced antioxidant enzyme activity in tissues and reduced total oxidative stress in plasma by the effect of Swietenia macrophylla king seeds in type II diabetes rats. International Journal of Herbal Medicine, 1(6), 31-36.
Edward (2017). Plant-Based Supplements: The New Frontier of Supplements. Retrieved from https://www.globalhealingcenter.com/natural-health/plantbased- supplements/.
Egwuche, R. U., Odetola, A. A., & Erukainure, O. L. (2011). Preliminary investigation into the chemical properties of Peperomia pellucida L. Research Journal of Phytochemistry, 1-6.
Ezhilarasan, D., Sokal, E., Karthikeyan, S., & Najimi, M. (2014). Plant derived antioxidants and antifibrotic drugs: Past, present and future. Journal of Coastal Life Medicine, 2(9), 738-745.
Farah Amna, O., Nooraain, H., Noriham, A., Azizah, A. H., & Husna, R. N. (2013). Acute and oral subacute toxicity study of ethanolic extract of Cosmos caudatus leaf in Sprague Dawley rats. International Journal of Bioscience, Biochemistry and Bioinformatics, 3(4), 301-305.
Fehmer. D. & Kiupel, I (2016). The Senate Commission on Animal Protection and Experimentation: Animal Experimentation in Research (pp. 11). Berlin: Deutsche Forschungsgemeinschaft (DFG, German Research Foundation).
Florence, N. T., Huguette, S. T. S., Hubert, D. J., Raceline, G. K., Desire, D. D. P., Pierre, K., et al (2017). Aqueous extract of Peperomia pellucida (L.) HBK accelerates fracture healing in Wistar rats. BMC Complementary and Alternative Medicine, 17(188), 1-9.
Gautam, M. K. & Goel, R. K. (2014). Toxicological study of Ocimum sanctum Linn leaves: Hematological, biochemical, and histopathological studies. Journal of Toxicology, 1-9.
Geetha, T. S. & Geetha, N. (2014). Phytochemical screening, quantitative analysis of primary and secondary metabolites of Cymbopogan citratus (DC) Stapf. leaves from Kodaikanal hills, Tamilnadu. International Journal of PharmTech Research, 6(2), 521-529.
Ghan, S. Y., Chin, J. H., Thoo, Y. Y., Yim, H. S., & Ho, C. W. (2016). Acute oral toxicity study of Aquilaria crassna and [alpha]-tocopherol in mice. International Journal of Pharmaceutical Sciences and Research, 7(4), 1456-1461.
Gandhare, B., Kavimani, S., & Rajkapoor, B. (2013). Acute and subacute toxicity study of methanolic extract of Ceiba pentandra (Linn.) Gaertn. on rats. Journal of Scientific Research, 5(2), 315-324.
Gini, T. G. & Jothi, G. J. (2013). Preliminary phytochemical screening for active compounds in the whole plant extract of Peperomia pellucida (Linn.) HBK (Piperaceae) and Marsilea quadrifolia Linn.(Marsileaceae). International Journal of Pharmacognosy and Phytochemical Research, 5(3), 200-214.
Gospodaryov, D. & Lushchak, V. (2012). Oxidative stress: cause and consequence of diseases. Retrieved from: https://www.intechopen.com/books/oxidative-stressand- diseases/oxidative-stress-as-a-cause-and-consequence-of-diseases.
Governo, A. C. (2004). The effects of the flavonoids quercetin and genistein on the antioxidant enzymes Cu, Zn superoxide dismutase, glutathione peroxidase, and glutathione reductase in male Sprague-Dawley rats (Master dissertation, Florida State University). Retrieved from https://pdfsecret.com/queue/theeffect- of-the-flavonoids-quercetin-and-genistein-on-the-antioxidant- _5a1df584d64ab217dbbfc8f0_pdf?queue_id=-1
Habig, W. H., Pabst, M. J., & Jakoby, W. B. (1974). Glutathione S-transferases the first enzymatic step in mercapturic acid formation. Journal of Biological Chemistry, 249(22), 7130-7139.
Habsah Mohamad, Yosie Andriani, Kamariah Bakar, Chow, C. S., Syamsumir, D. F., Asmah Alias et al (2015). Effect of drying method on anti-microbial, antioxidant activities and isolation of bioactive compounds from Peperomia pellucida (L) Hbk. Journal of Chemical and Pharmaceutical Research, 7(8), 578-584.
Hamzah, R. U., Odetola, A. A., Erukainure, O. L., & Oyagbemi, A. A. (2012). Peperomia pellucida in diets modulates hyperglyceamia, oxidative stress and dyslipidemia in diabetic rats. Journal of Acute Disease, 135-140.
Han, C. T., Kim, M. J., Moon, S. H., Jeon, Y. R., Hwang, J. S., Nam, C., et al (2015). Acute and 28-day subacute toxicity studies of hexane extracts of the roots of Lithospermum erythrorhizon in Sprague-Dawley rats. Toxicological research, 31(4), 403-414.
Hara, C. (2014). Study of treated and untreated oil-based drilling waste exposure in Atlantic salmon (Salmo salar) using a biomarker approach: EROD and oxidative stress parameters (Master's thesis, University of Stavanger, Norway). Retrieved from https://brage.bibsys.no/xmlui/bitstream/id/209878/Masters.
Hartati, S., Angelina, M., Dewiyanti, I. D., & Meilawati, L. (2015). Isolation and characterization compounds from hexane and ethyl acetate fractions of Peperomia pellucida L. Journal of Tropical Life Science, 5(3), 117-122.
Hasani, P., Yasa, N., Vosough-Ghanbari, S., Mohammadirad, A., Dehghan, G., & Abdollahi, M. (2007). In vivo antioxidant potential of Teucrium polium, as compared to α-tocopherol. Acta pharmaceutica, 57, 123-129.
Hassan, L. E. A., Hasnah Mohd Sirat,, Yagi, S. M. A., Koko, W. S., & Abdelwahab, S. I. (2011). In vitro antimicrobial activities of chloroformic, hexane and ethanolic extracts of Citrullus lanatus var. citroides (Wild melon). Journal of Medicinal Plants Research, 5(8), 1338-1344.
Horwith, B. & Lindsay, K. (1999). In Towle, J. (Eds.) A biodiversity profile of St. Kitts and Nevis (pp. 10) St. Johns, Antigua: Island Resources Foundation.
Hosseinzadeh, S., Jafarikukhdan, A., Hosseini, A., & Armand, R. (2015). The application of medicinal plants in traditional and modern medicine: A review of Thymus vulgaris. International Journal of Clinical Medicine, 6, 635-642.
Hotra, L., Kolasky, E., & Voss, J. (2003). Abiotic factors influencing the abundance and distribution of marsh marigolds along Carp Creek. Retrieved from https://deepblue.lib.umich.edu/bitstream/handle/2027.42/54986/3427.pdf?seque nce=1
Htet, Y. M., Sabai, & Khaing, M. M. (2016). Botanical studies and phytochemical screening of Peperomia pellucida (L.) kunth (Thit-Yay-Gyi). Hinthada University Research Journal, 7(1), 106-111.
Huang, D., Ou, B., & Prior, R. L. (2005). The chemistry behind antioxidant capacity assays. Journal of Agricultural and Food Chemistry, 53(6), 1841-1856.
Husain, N. & Kumar, A. (2012). Reactive oxygen species and natural antioxidants: A review. Advances in Bioresearch, 17(1), 24-38.
Ibibia, E. K. T. (2012). Phytochemical and antimicrobial analyses of extracts of Peperomia pellucida (L). Journal of Pharmacy Research Vol, 5(5), 2934-2937.
Ibrahim Jantan (2004). Medicinal plant research in Malaysia: Scientific interests and advances. Jurnal Sains Kesihatan Malaysia, 2(2), 27-46.
Ibrahim Jantan (2006). The scientific values of Malaysian herbal products. Jurnal Sains Kesihatan Malaysia, 4(1), 59-70.
Igwe, O. U. & Mgbemena, N.M. A. (2014). Chemical investigations and antibacterial activity of the leaves of Peperomia pellucida L. HBK (Piperaceae). Asian Journal of Chemical and Pharmaceutical Research, 2(1), 78-86.
Ingebrigtsen, K. (2010). Main plant poisonings in livestock in the Nordic countries. In Bernholf, A (Eds.), Bioactive Compounds in Plants-Benefits and Risks for Man and Animals (pp. 31). Norway: The Norwegian Academy of Science and Letters.
Inkielewicz, I., Rogowska, M., & Krechniak, J. (2006). Lipid peroxidation and antioxidant enzyme activity in rats exposed to fluoride and ethanol. Fluoride, 39(1), 53-59.
Jain, A., Bhatt, S., & Dhyani, S. (2012). Phytochemical screening of secondary metabolites of Ziziphus Mauritiana Lam. bark. International Journal of Current Pharmaceutical Research, 4(3), 156-159.
Jaradat, N., Hussen, F., & Al Ali, A. (2015). Preliminary phytochemical screening, quantitative estimation of total flavonoids, total phenols and antioxidant activity of Ephedra alata Decne. Journal of Materials and Environmental Sciences, 6(6), 1771-1778.
Jayaprakasha, G. K., Singh, R. P., & Sakariah, K. K. (2001). Antioxidant activity of grape seed (Vitis vinifera) extracts on peroxidation models in vitro. Food chemistry, 73(2001), 285-290.
Jeyaseelan, E. C, Tharmila, S., Sathiyaseelan, V., & Niranjan, K. (2012). Antibacterial activity of various solvent extracts of some selected medicinal plants present in Jaffna Peninsula. International Journal of Pharmaceutical & Biological Archive, 3(4), 792-796.
Johanningsmeier, S. D. & Harris, G. K. (2011). Pomegranate as a functional food and nutraceutical source. Annual Review of Food Science and Technology, 2, 181- 201.
Johnson, D. B., Shringi, B. N., Patidar, D. K., Chalichem, N. S. S., & Javvadi, A. K. (2011). Screening of antimicrobial activity of alcoholic & aqueous extract of some indigenous plants. Indo-Global Journal of Pharmaceutical Sciences, 1(2), 186-193.
Jonsson, M., Jestoi, M., Nathanail, A. V., Kokkonen, U. M., Anttila, M., Koivisto, P., et al. (2013). Application of OECD Guideline 423 in assessing the acute oral toxicity of moniliformin. Food and Chemical Toxicology, 53, 27-32.
Jothy, S. L., Zuraini Zakaria, Chen, Y., Yee, L. L., Latha, L. Y., & Sasidharan, S. (2011). Acute oral toxicity of methanolic seed extract of Cassia fistula in mice. Molecules, 16, 5268-5282.
Kabel, A. M. (2014). Free radicals and antioxidants: Role of enzymes and nutrition. World Journal of Nutrition and Health, 2(3), 35-38.
Kang, M., Kim, D. W., Baek, Y. J., Moon, S. H., Jung, H. J., Song, Y. J., et al (2014). Dietary supplement use and its effect on nutrient intake in Korean adult population in the Korea national health and nutrition examination survey IV (2007–2009) data. European Journal of Clinical Nutrition, 68, 804-810.
Kartika, I. G. A. A., Insanu, M., Safitri, D., Putri, C. A., & Adnyana, I. K. (2016). New update: Traditional uses, phytochemical, pharmacological and toxicity review of Peperomia pellucida (l.) Kunth. Newsletter, 2, 30-43.
Kasote, D. M., Katyare, S. S., Hegde, M. V., & Bae, H. (2015). Significance of antioxidant potential of plants and its relevance to therapeutic applications. International Journal of Biological Sciences, 11(8), 982-991.
Kathe, W. (2006). Revision of the 'guidelines on the conservation of medicinal plants' by WHO, IUCN, WWF and TRAFFIC: Medicinal and Aromatic Plants, 109- 120.
Kaware Mangesh, K. (2013). Changes in liver and body weight of mice exposed to toxicant. International Research Journal of Science & Engineering, 1(3), 92-95.
Kennedy, D. O. & Wightman, E. L. (2011). Herbal extracts and phytochemicals: Plant secondary metabolites and the enhancement of human brain function. American Society for Nutrition,: 2, 32-50.
Khan, M. R. & Omoloso, A. D. (2002). Antibacterial activity of Hygrophila stricta and Peperomia pellucida. Fitoterapia, 73, 251-254.
Khan, A., Rahman, M., & Islam, S. (2008a). Antipyretic activity of Peperomia pellucida leaves in rabbit. Turkish Journal of Biology, 32, 37-41.
Khan, A., Rahman, M., & Islam, M. S. (2008b). Neuropharmacological effects of Peperomia pellucida leaves in mice. Daru, 16(1), 35-40.
Khan, A., Rahman, M., & Islam, M. S. (2010). Isolation and bioactivity of a xanthone glycoside from Peperomia pellucida. Life Sciences and Medicine Research. 2010, 1-10.
Khokra, S. L., Parashar, B., Dhamija, H. K., Chandel, A. & Rekha, C. (2011). A review describing various in vitro methods for evaluation of antioxidant activity. Asian Journal of Biochemical and Pharmaceutical Research, 4(1), 94- 99.
Kim, J. E., Nam, J. H., Cho, J. Y., Kim, K. S., & Hwang, D. Y. (2017). Annual tendency of research papers used ICR mice as experimental animals in biomedical research fields. Laboratory animal research, 33(2), 171-178.
Kim, J. W., Lee, S. H., Kim, J. E., Han, K. D., Kwack, T. E., Kim, B. S., et al (2016). The association between taking dietary supplements and healthy habits among Korean adults: Results from the fifth Korea national health and nutritional examination survey (2010–2012). Korean Journal of Family Medicine, 37(3), 182-187.
Kim, S., Song, Y., Lee, J. E., Jun, S., Shin, S., Wie, G. A., et al (2017). Total antioxidant capacity from dietary supplement decreases the likelihood of having metabolic syndrome in Korean adults. Nutrients, 9, 1-14.
Krishnamurthy, P. & Wadhwani, A. (2012). Antioxidant enzymes and human health. In El-Missiry, M. A. (Eds.), Antioxidant enzyme (pp. 3). Croatia: Intech Books and Journals.
Kumar, P., Suba, M., Ramireddy, V., & Srivinas Babu, P. (2014). Acute and sub- Acute (28-Day) oral toxicity studies of ethanolic extract of Celtis Timorensis leaves in rodents. Global Journal of Medical Research: B Pharma, Drug Discovery, Toxicology and Medicine, 14(3), 6-14.
Kumar, S. (2011). Free radicals and antioxidants: Human and food system. Advances in Applied Science Research, 2(1), 129-135.
Kumar, S. (2014). The important of antioxidant and their role in pharmaceutical science- A review. Asian Journal of Research in Chemistry and Pharmaceutical Sciences, 1(1), 27-44.
Lalitha, P., Sripathi, S. K., & Jayanthi, P. (2012). Acute toxicity study of extracts of Eichhornia Crassipes (Mart.) Solms. Asian Journal of Pharmaceutical and Clinical Research, 5(4), 59-61.
Lee, S. W., Sim, K. Y., Wendy, W., & Zulhisyam, A. K. (2016). Peperomia pellucida leaf extract as immunostimulator in controlling motile aeromonad septicemia due to Aeromonas hydrophila in red hybrid tilapia, Oreochromis spp. farming. Veterinary World, 9(3), 231-234.
Liang, N. & Kitts, D. D. (2014). Antioxidant property of coffee components: Assessment of methods that define mechanisms of action. Molecules, 19, 19180-19208.
Liu, R. H. (2004). Potential synergy of phytochemicals in cancer prevention: mechanism of action. Paper presented at the International Research Conference on Food, Nutrition, and Cancer. Retrieved from https://www.ncbi.nlm.nih.gov/pubmed/15570057.
Lobo, V., Patil, A., Phatak, A., & Chandra, N. (2010). Free radicals, antioxidants and functional foods: Impact on human health. Pharmacognosy reviews, 4(8), 118- 126.
Loc, N. H., Bach, N. H., Kim, T. G., & Yang, M. S. (2010). Tissue culture and expression of Escherichia coli heat-labile enterotoxin B subunit in transgenic Peperomia pellucida. Protein Expression and Purification, 72, 82-86.
Lukaszewicz-Hussain, A. & Moniuszko-Jakoniuk, J. (2004). Liver catalase, glutathione peroxidase and reductase activity, reduced glutathione and hydrogen peroxide levels in acute intoxication with chlorfenvinphos, an organophosphate insecticid. Polish Journal of Environmental Studies, 13(3). 303-309.
Mahantesh, S. P., Gangawane, A. K., & Patil, C. S. (2012). Free radicals, antioxidants, diseases and phytomedicines in human health: Future perspects. World Research Journal of Medicinal & Aromatic Plants, 1(1), 6-10.
Maisarah, A. M., Nurul Amira, B., Asmah, R., & Fauziah, O. (2013). Antioxidant analysis of different parts of Carica papaya. International Food Research Journal, 20(3), 1043-1048.
Majumder, P. (2011). Phytochemical, pharmacognostical and physicochemical standardization of Peperomia pellucida (L.) HBK. Stem. International Journal of Comprehensive Pharmacy, 8(6), 1-4.
Majumder, P., Abraham, P., & Satya, V. (2011). Ethno-medicinal, phytochemical and pharmacological review of an amazing medicinal herb Peperomia pellucida (L.) HBK. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 2(4), 358-364.
Majumder, P. (2012). Evaluation of taxo-chemical standardization and quality control parameters of Peperomia pellucida (Family: Piperaceae): A multi valuable medicinal herb. Journal of Pharmaceutical Science Innovation, 1(6), 7-12.
Majumder, P. & Arun K. K. V., (2011). Establishment of quality parameters and pharmacognostic evaluation of leaves of Peperomia pellucida (L.) HBK. International Journal of Pharmacy and Pharmaceutical Sciences, 3(5), 375-378.
Mamedov, N. (2012). Medicinal plants studies: History, challenges and prospective. Medicinal & Aromatic Plants, 1(8), 1000e133.
Mammadov, R., Ili, P., Ertem Vaizogullar, H., & Afacan Makasc?, A. (2011). Antioxidant activity and total phenolic content of Gagea fibrosa and Romulea ramiflora. Iranian Journal of Chemistry and Chemical Engineering, 30(3), 57- 62.
Manoharan, S. (2013). Synergistic activity of chloroform extract of Durio zibethinus wood bark with penicillin g against Staphylococcus aureus. International Journal of Biological & Medical Research, 4(2), 3025-3027.
Marklund, S. & Marklund, G. (1974). Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Europian Journal of Biochemistry, 47, 469-474.
Marxen, K., Vanselow, K. H., Lippemeier, S., Hintze, R., Ruser, A., & Hansen, U. P. (2007). Determination of DPPH radical oxidation caused by methanolic extracts of some microalgal species by linear regression analysis of spectrophotometric measurements. Sensors, 7, 2080-2095.
Mathan, S., Smith, A. A., Daran, S. S., Kumaran, J., & Prakash, S. (2015). Chronis and sub-acute toxicity study of the n-butanol extract of the marine sediment fungus Aspergillus protuberus spl. International Research Journal of Pharmacy, 6(8), 552-558.
McKenzie R. H. (2003). Soil pH and plant nutrients. Agri-Facts: Practicle Information for Alberta’s Agriculture Industry. Retrieved from https://www1.agric.gov.ab.ca/$department/deptdocs.nsf/all/agdex6607/$file/soil ph.pdf?OpenElement.
Meite, S., N’guessan, J. D., Bahi, C., Yapi, H. F., Djaman, A. J., & Guina, F. G. (2009). Antidiarrheal activity of the ethyl acetate extract of Morinda morindoides in rats. Tropical Journal of Pharmaceutical Research, 8(3). 201- 207.
Melo, A., Guimarães, E. F., & Alves, M. (2016). Synopsis of the genus Peperomia Ruiz & Pav.(Piperaceae) in Roraima State, Brazil. Hoehnea, 43(1), 119-134.
Menon, N. (2014). Metabolic effects of the consumption of aqueous Kalanchoe pinnata preparation in streptozotocin-induced diabetic rats. (Master dissertation,Texas A&M University). Retrieved from https://www.google.com/search?q=Metabolic+Effects+of+the+Consumption+of +Aqueous+Kalanchoe+pinnata+Preparation+in+Streptozotocin- Induced+Diabetic+Rats&oq=Metabolic+Effects+of+the+Consumption+of+Aqu eous+Kalanchoe+pinnata+Preparation+in+Streptozotocin- Induced+Diabetic+Rats&aqs=chrome..69i57j69i60.1920j0j1&sourceid=chrome &ie=UTF-8#
Mensah, J. K., Ihenyen, J. O., & Okhiure, M. O. (2013). Nutritional, phytochemical and antimicrobial properties of two wild aromatic vegetables from Edo State. Journal of Natural Product and Plant Resources, 3(1), 8-14.
Misra, A., Srivastava, S., Verma, S., & Rawat, A. J. S. (2015). Nutritional evaluation, antioxidant studies and quantification of poly phenolics, in Roscoea purpurea tubers. BMC research notes, 8(324), 1-7.
Mohamed, A. A., Khalil, A. A., & El-Beltagi, H. E. (2010). Antioxidant and antimicrobial properties of kaff maryam (Anastatica hierochuntica) and doum palm (Hyphaene thebaica). Grasas Y Aceites, 61(1), 67-75.
Mohiseni, M. (2017). Medicinal herbs, strong sources of antioxidant in aquaculture: A Mini Review. Modern Applications for Pharmacy & Pharmacology, 1(1), 1-5.
Morilla, L. J. G., Sumaya, N. H. N., Rivero, H. I., & Madamba, M. R. S. B. (2014). Medicinal plants of the Subanens in Dumingag, Zamboanga del Sur, Philippines. Paper presented at the International Conference on Food, Biological and Medical Sciences, Bangkok, Thailand. Retrieved from http://iicbe.org/upload/4970C0114577.pdf.
Muddappur, U. M., More, S., Karekar, T., Mungi, H., Thakkannavar, S., & Savadatti, N. (2014). Evaluation of acute and sub-acute toxicity effect of ethanol extract from Caesalpinia bonducella seed kernel. Journal of Pharmacognosy and Phytochemistry, 3(3), 120-124.
Muhammad Kifayatullah, Mohd Shahimi Mustafa, Sengupta, P., Sarker, Md. M. R., Das, A., & Das, S. K (2015). Evaluation of the acute and sub-acute toxicity of the ethanolic extract of Pericampylus glaucus (Lam.) Merr. in BALB/c mice. Journal of Acute Disease, 4(4), 309-315.
Muhammad Shahzad Aslam & Muhammad Syarhabil Ahmad (2016). Worldwide importance of medicinal plants: Current and historical perspectives. Recent Advances in Biology and Medicine, 2, 88-93.
Muralidhara, S., Ramanathan, R., Mehta, S. M., Lash, L. H., Acosta, D., & Bruckner, J. V. (2001). Acute, subacute, and subchronic oral toxicity studies of 1, 1- dichloroethane in rats: Application to risk evaluation. Toxicological Sciences, 64, 135-145.
Mutee, A. F., Salhimi, S. M., Yam, M. F., Lim, C. P., Abdullah, G. Z., Ameer, O. Z., et al (2010). In vivo anti-inflammatory and in vitro antioxidant activities of Peperomia pellucida. International Journal of Pharmacology, 6(5), 686-690.
Nagananda, G. S. & Satishchandra, N. (2013). Antimicrobial activity of cold and hot successive pseudobulb extracts of Flickingeria nodosa (Dalz.) Seidenf. Pakistan Journal of Biological Sciences, 16(20), 1189-1193.
Narayanamoorthi, V., Vasantha, K., Rency, R. C., & Maruthasalam, R. R. A. A. (2015). GC MS determination of bioactive components of Peperomia pellucida (L.) Kunth. Bioscience Discovery, 6(2), 83-88.
Naskar, S., Islam, A., Mazumder, U. K., Saha, P., Haldar, P. K., & Gupta, M. (2009). In vitro and in vivo antioxidant potential of hydromethanolic extract of Phoenix dactylifera fruits. Journal of Scientific Research, 2(1), 144-157.
Nayak, B. S. & Pereira, L. M. P. (2006). Catharanthus roseus flower extract has wound-healing activity in Sprague Dawley rats. BMC Complementary and Alternative Medicine, 6(1), 1-6.
Ncube, N. S., Afolayan, A. J., & Okoh, A. I. (2008). Assessment techniques of antimicrobial properties of natural compounds of plant origin: Current methods and future trends. African Journal of Biotechnology, 7(12), 1797-1806.
Ndhlala, A. R., Moyo, M., & Staden, J. (2010). Natural antioxidants: Fascinating or mythical biomolecules?. Molecules, 15, 6905-6930.
Nile, S. H. & Park, S. W. (2014). Edible berries: Bioactive components and their effect on human health. Nutrition, 30(2014), 134-144.
Nostro, A., Germano, M. P., D’angelo, V., Marino, A., & Cannatelli, M. A. (2000). Extraction methods and bioautography for evaluation of medicinal plant antimicrobial activity. Letters in Applied Microbiology, 30, 379-384.
Nur Kartinee Kassim, Mawardi Rahmani, Amin Ismail, Aminah Abdullah & Khalid Hamid Musa (2012). The Measurement of Antioxidant Capacity of Melicope glabra by ORAC-FL and DPPH and its Chemical Constituents. Paper presented in 3rd International Conference on Chemistry and Chemical Engineering, Singapore. Retrieved from http://www.ipcbee.com/vol38/007-ICCCE2012- A011.pdf.
Nur Kartinee Kassim, Mawardi Rahmani, Amin Ismail, Mohd Aspollah Sukari, Ee, G. C. L., Nadiah Md Nasir et al., (2013). Antioxidant activity-guided separation of coumarins and lignan from Melicope glabra (Rutaceae). Food chemistry, 139(1-4), 87-92.
Nwokocha, C. R., Owu, D. U., Kinlocke, K., Murray, J., Delgoda, R., Thaxter, K., et al (2012). Possible mechanism of action of the hypotensive effect of Peperomia pellucida and interactions between human cytochrome P450 enzymes. Medical & Aromatic Plants, 1(4), 1000105.
Nyamai, D. W., Arika, W., Ogola, P. E., Njagi, E. N. M., & Ngugi, M. P. (2016). Medicinally important phytochemicals: An untapped research avenue. Research and Reviews: Journal of Pharmacognosy and Phytochemistry, 4(1), 35-49.
OECD (2008). Guidelines for the Testing of Chemicals 407: Repeated Dose 28-Day Oral Toxicity Study in Rodents.
Oghenesuvwe, E. E., Ekene, N. E., & Lotanna, A. D., (2014). Guidelines on dosage calculation and stock solution preparation in experimental animals’ studies. Journal of Natural Sciences Research, 4(18), 100-106.
Ogbonnia, S. O., Olayemi, S. O., Anyika, E. N., Enwuru, V. N., & Poluyi, O. O. (2009). Evaluation of acute toxicity in mice and subchronic toxicity of hydroethanolic extract of Parinari curatellifolia Planch (Chrysobalanaceae) seeds in rats. African Journal of Biotechnology, 8(9), 1800-1809.
Ogbonnia, S. O., Mbaka, G. O., Anyika, E. N., Osegbo, O. M., & Igbokwe, N. H. (2010). Evaluation of acute toxicity in mice and subchronic toxicity of hydroethanolic extract of Chromolaena odorata (L.) King and Robinson (Fam. Asteraceae) in rats. Agriculture and Biology Journal of North America, 1(5), 859-865.
Ognjanovi?, B. I., Djordjevi?, N. Z., Mati?, M. M., Obradovi?, J. M., Mladenovi?, J. M., Štajn, A. Š., et al (2012). Lipid peroxidative damage on cisplatin exposure and alterations in antioxidant defense system in rat kidneys: A possible protective effect of selenium. International Journal of Molecular Sciences, 13, 1790-1803.
Ojo, O. O., Ajayi, S. S., & Owolabi, L. O. (2012). Phytochemical screening, antinutrient composition, proximate analyses and the antimicrobial activities of the aqueous and organic extracts of bark of Rauvolfia vomitoria and leaves of Peperomia pellucida. International Research Journal of Biochemistry and Bioinformatics, 2(6), 127-134.
Okwu, M. U., Okorie, T. G., Agba, M. I., Ayinde, B. A., & Umumarongie, H. O. (2014). Comparative anti-MRSA activities of seven selected Nigerian medicinal plants and phytochemical constituents of Piper guineense (Schum and Thonn.), Curculigo pilosa (Schum and Thonn.) and Chromolaena odorata (King and Robinson). Journal of Pharmacy and Biological Sciences, 9(5), 7-13.
Oloyede, G. K., Onocha, P. A., & Olaniran, B. B. (2011). Phytochemical, toxicity, antimicrobial and antioxidant screening of leaf extracts of Peperomia pellucida from Nigeria. Advances in Environmental Biology, 5(12), 3700-3709.
Olayinka, E. T., Ore, A., Adeyemo, O. A., Ola, O. S., Olotu, O. O., & Echebiri, R. C. (2015). Quercetin, a flavonoid antioxidant, ameliorated procarbazine-induced oxidative damage to murine tissues. Antioxidants, 4, 304-321.
Omaye, S. & Elsayed, N. (2012). Antioxidants to supplement or not to supplement that is the question. Antioxidants, 1, 1-3.
Ong, H. C., Ruzalila, B. N., & Milow, P. (2011). Traditional knowledge of medicinal plants among the Malay villagers in Kampung Tanjung Sabtu, Terengganu, Malaysia. Indian Journal of Traditional Knowledge, 10(3), 460-465.
Ong, H. C., Lina, E., & Milow, P. (2012). Traditional knowledge and usage of medicinal plants among the Semai Orang Asli at Kampung Batu 16, Tapah, Perak, Malaysia. Ethno Medicine, 6(3), 207-211.
Ooi, D. J., Iqbal, S., & Maznah Ismail (2012). Proximate composition, nutritional attributes and mineral composition of Peperomia pellucida L.(Ketumpangan Air) grown in Malaysia. Molecules, 17, 11139-11145.
Oter, S., Jin, S., Cucullo, L., & Dorman, H. D. (2012). Oxidants and antioxidants: Friends or foes?. Oxidants and Antioxidants in Medical Science, 1(1), 1-4.
Ou, B., Hampsch-Woodill, M., & Prior, R. L. (2001). Development and validation of an improved oxygen radical absorbance capacity assay using fluorescein as the fluorescent probe. Journal of Agricultural and Food Chemistry, 49, 4619-4626.
Pan, S. Y., Litscher, G., Gao, S. H., Zhou, S. F., Yu, Z. L., Chen, H. Q., et al (2014). Historical perspective of traditional indigenous medical practices: The current renaissance and conservation of herbal resources. Evidence-Based Complementary and Alternative Medicine, 2014, 1-20.
Pandey, A. & Tripathi, S. (2014). Concept of standardization, extraction and pre phytochemical screening strategies for herbal drug. Journal of Pharmacognosy and Phytochemistry, 2(5), 115-119.
Pappachen, L. K. & Chacko, A. (2013). In-vitro antioxidant activity and determination of total phenolic, flavonoid contents of Peperomia pellucida Linn. American Journal of Pharmacy and Health Research, 1(7), 93-101.
Parasuraman, S. (2011). Toxicological screening. Journal of Pharmacology & Pharmacotherapeutics, 2(2), 74-80.
Parekh, J., Jadeja, D., & Chanda, S. (2005). Efficacy of aqueous and methanol extracts of some medicinal plants for potential antibacterial activity. Turkish Journal of Biology, 29, 203-210.
Pham-Huy, L. A., He, H., & Pham-Huy, C. (2008). Free radicals, antioxidants in disease and health. International Journal of Biomedical Science, 4(2), 89-96.
Phongtongpasuk, S. & Poadang, S. (2014). Extraction of antioxidants from Peperomia pellucida L. Kunth. Thammasat International Journal of Science & Technology, 19(3), 38-43.
Poljsak, B., Šuput, D., & Milisav, I. (2013). Achieving the balance between ROS and antioxidants: When to use the synthetic antioxidants. Oxidative Medicine and Cellular Longevity, 2013, 1-11.
Porwal, M., Khan, N. A., & Maheshwari, K. K. (2017). Evaluation of acute and subacute oral toxicity induced by ethanolic extract of Marsdenia tenacissima leaves in experimental rats. Scientia pharmaceutica, 85, 1-11.
Prakash, A., Rigelhof, F., & Miller, E. (2018). Medallion laboratories analytical progress: Antioxidant activity. Retrieved from https://www.academia.edu/4772227/ANALYTICAL_PROGRESS_Medallion_Labor atories_Antioxidant_Activity_WHAT_ARE_ANTIOXIDANTS.
Prior, R. L., Wu, X., & Schaich, K. (2005). Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. Journal of Agricultural and Food Chemistry, 53, 4290-4302.
Priyadi, H., Takao, G., Rahmawati, I., Supriyanto, B., Nursal, W. I., & Rahman, I. (2010). Five hundred plant species in Gunung Halimun Salak National Park, West Java: A checklist including Sundanese names, distribution and use (pp. 143). Indonesia: Centre for International Forestry Research.
Pulak, M. & NarayanaMurthy, G. (2011). Physicochemical standardization and pharmacognostical investigation of Zyziphus oenoplia (L.) Mill. root. Asian Journal of Biomedical and Pharmaceutical Sciences, 1(1), 28-31.
Race, S. (2009). Antioxidants: The truth about BHA, BHT, TBHQ and other antioxidants used as food additives (pp. 17, 22). United Kingdom: Tigmor Books.
Radovanovi?, T. B., Borkovi?-Miti?, S. S., Perendija, B. R., Despotovi?, S. G., Pavlovi?, S. Z., Caki?, P. D., et al (2010). Superoxide dismutase and catalase activities in the liver and muscle of barbel (Barbus barbus) and its intestinal parasite (Pomphoryinchus laevis) from the Danube river, Serbia. Archives of Biological Sciences, 62(1), 97-105.
Rafieian-Kopaei, M. (2012). Medicinal plants and the human needs. Journal of HerbMed Pharmacology, 1(1), 1-2.
Raina, H., Soni, G., Jauhari, N., Sharma, N., & Bharadvaja, N. (2014). Phytochemical importance of medicinal plants as potential sources of anticancer agents. Turkish Journal of Botany, 38, 1027-1035.
Rajeshkumar, D. (2010). Evaluation of Antioxidant Property and Toxicological Assessment of Polyalthia Longifolia var. Pendula Leaf (Doctoral dissertation, Saurashtra University). Retrieved from http://etheses.saurashtrauniversity.edu/567/1/dave_ra_thesis_biochemistry_red.. pdf.
Rani, K. (2017). Role of antioxidants in prevention of diseases. Journal of Applied Biotechnology & Bioengineering, 4(1), 00091.
Rao, S. P., Kalva, S., Yerramilli, A., & Mamidi, S. (2011). Free radicals and tissue damage: Role of antioxidants. Free Radicals and Antioxidants, 1(4), 2-7.
Rao, U. S. M., Muhammad Abdulrrazak, & Khamsah Suryati Mohd (2016). Phytochemical screening, total flavonoid and phenolic content assays of various solvent extracts of tepal of Musa paradisiaca. Malaysian Journal of Analytical Sciences, 20(5), 1181-1190.
Rasool Hassan, B. A. (2012). Medicinal plants (importance and uses). Pharmaceutica Analytica Acta, 3(10), 1000e139.
Ritchie, G. S. P., & Dolling, P. J. (1985). The role of organic matter in soil acidification. Australian Journal of Soil Research, 23(4), 569-576.
Rojas-Martínez, R., Arrieta, J., Cruz-Antonio, L., Arrieta-Baez, D., Velázquez- Méndez, A. M., & Sánchez-Mendoza, M. E. (2013). Dillapiole, isolated from Peperomia pellucida, shows gastroprotector activity against ethanol-induced gastric lesions in Wistar rats. Molecules, 18, 11327-11337.
Roslida, A. H. & Noor Aini, Z. (2009). Evaluation of gastroprotective effects of the ethanolic extract of Peperomia pellucida (L) Kunth. Pharmacology Online, 2, 678-686.
Roy, S., Ukil, B., & Lyndem, L. M. (2016). Acute and sub-acute toxicity studies on the effect of Senna alata in Swiss Albino mice. Cogent Biology, 2, 1-11.
Royal Botanic Gardens Kew. (2017), State of the world’s plant: Describing the whole plant. Retrieved from https://stateoftheworldsplants.com/2017/report/SOTWP_2017.pdf.
Sahreen, S., Khan, M. R., Khan, R. A., & Shah, N. A. (2013). Estimation of flavoniods, antimicrobial, antitumor and anticancer activity of Carissa opaca fruits. BMC Complementary and Alternative Medicine, 13, 1-7.
Salim, A. A., Chin, Y. W. & Kinghorn, A. D. (2008). Drug discovery from plants. In Ramawat, K. G & Merillon, J. M. (Eds.), Bioactive molecules and medicinal plants (pp 1),Ohio: Springer.
Sangeetha, J. & Vijayalakshmi, K. (2011). Determination of bioactive components of ethyl acetate fraction of Punica granatum rind extract. International Journal of Pharmaceutical Sciences and Drug Research, 3(2), 116-122.
Saripah Salbiah Syed Abdul Azziz, Munirah Abdul Talip, Wong, C. F., Hasimah Alimon, Norlaili Abu Bakar, Wan Rusmawati Wan Mahamod, et al (2015). Determination of Malaysian herbs and spices as biopreservative agents in food products. American Journal of Plant Sciences, 6, 718-724.
Sarma, A. D., Mallick, A. R., & Ghosh, A. K. (2010). Free radicals and their role in different clinical conditions: An overview. International Journal of Pharma Sciences and Research, 1(3), 185-192.
Saw, L. G., Chua, L. S. L., Suhaida, M., Yong, W. S. Y., & Hamidah, M. (2010). Conservation of some rare and endangered plants from Peninsular Malaysia. Kew Bulletin, 65(4), 681-689.
Saxena, M., Saxena, J., & Pradhan, A. (2012). Flavonoids and phenolic acids as antioxidants in plants and human health. International Journal of Pharmaceutical Sciences Review and Research, 16(2), 130-134.
Saxena, M., Saxena, J., Nema, R., Singh, D., & Gupta, A. (2013). Phytochemistry of medicinal plants. Journal of pharmacognosy and phytochemistry, 1(6), 168-182.
Schneider, C. & Segre, T. (2009). Green tea: potential health benefits. American family physician, 79(7), 591-594.
Sekar, M., Muhammad Zulhilmi Abdullah, Ahmad Yasser Hamdi Nor Azlan, Siti Nabila Nasir, Zahida Zakaria, & Mohd Shafiq Abdullah. (2014). Ten commonly available medicinal plants in Malaysia used for the treatment of diabetes-A review. Asian Journal of Pharmaceutical and Clinical Research, 7(1), 1-5.
Shahid-Ud-Daula, A. F. M., & Basher, M. A. (2009). Phytochemical screening, plant growth inhibition, and antimicrobial activity studies of Xylocarpus granatum. Malaysian Journal Pharmaceutical Sciences, 7(1), 9-21.
Shah, M. A., Sarker, M. M. R., & Gousuddin, M. (2016). Toxicity study of Brassica oleracea italic extracts in Sprague Dawley (SD) rats. International Journal of Pharmacognosy and Phytochemical Research, 8(5), 735-741.
Shalaby, E. A. & Shanab, S. M. M. (2013). Antioxidant compounds, assays of determination and mode of action. African Journal of Pharmacy and Pharmacology, 7(10), 528-539.
Sharwan, G., Jain, P., Pandey, R., & Shukla, S. S. (2015). Toxicity profile of traditional herbal medicine. Journal of Ayurvedic and Herbal medicine, 1(3), 81-90.
Sheah, Y. G., Jin, H. C., Yin, Y. T., Hip, S. Y., & Chun, W. H. (2016). Acute oral toxicity study of Aquilaria crassna and α-tocopherol in mice. International Journal of Pharmaceutical Sciences and Research, 7(4), 1456-1461.
Sheikh, H., Sikder, S., Paul, S. K., Hasan, A. M. R., Rahaman, M. M., & Kundu, S. P. (2013). Hypoglycemic, anti-inflammatory and analgesic activity of Peperomea pellucida (L.) Hbk (Piperaceae). International Journal of Pharmaceutical Sciences and Research, 4(1), 458-463.
Shekhawat, M. S., & Manokari, M. (2015). Direct organogenesis for mass propagation of Peperomia pellucida L. an epiphytic medicinal herb. World Scientific News, 23, 24-34.
Shimamura, T., Sumikura, Y., Yamazaki, T., Tada, A., Kashiwagi, T., Ishikawa, H., et al (2014). Applicability of the DPPH assay for evaluating the antioxidant capacity of food additives–inter-laboratory evaluation study–. Analytical Sciences, 30, 717-721.
Singh, R. (2016). Chemotaxonomy: A tool for plant classification. Journal of Medicinal Plants, 4(2), 90-93.
Siramon, P. & Ohtani, Y. (2007). Antioxidative and antiradical activities of Eucalyptus camaldulensis leaf oils from Thailand. Journal of Wood Science, 53, 498-504.
Siti Suriani Arsad, Norhaizan Mohd Esa, Hazilawati Hamzah, & Fauziah Othman. (2013). Evaluation of acute, subacute and subchronic oral toxicity of Rhaphidophora decursiva (Roxb.) Schott extract in male Sprague Dawley rats. Journal of Medicinal Plants Research, 7(41), 3030-3040.
Sivakrishnan, S. & KottaiMuthu. A. (2013). In vivo antioxidant activity of ethanolic extract of aerial parts of Albizia procera roxb (benth.) against paracetamol induced liver toxicity on wistar rats. Journal of Pharmaceutical Sciences and Research, 5(9), 174-177.
Song, S., Youn, J., Lee, Y. J., Kang, M., Hyun, T., Song, Y., et al (2017). Dietary supplement use among cancer survivors and the general population: A nationwide cross-sectional study. BMC cancer, 17(891), 1-12.
Sultana, B., Anwar, F., & Ashraf, M. (2009). Effect of extraction solvent/technique on the antioxidant activity of selected medicinal plant extracts. Molecules, 14, 2167-2180.
Susilawati, Y., Nugraha, R., Muhtadi, A., Soetardjo, S., & Supratman, U. (2015). (S)- 2-Methyl-2-(4-methylpent-3-enyl)-6-(propan-2-ylidene)-3, 4, 6, 7- tetrahydropyrano [4, 3-g] chromen-9 (2H)-one. Molbank, 1-6.
Sussa, F. V., Damatto, S. R., Alencar, M. M., Mazzilli, B. P., & Silva, P. S. C. (2013). Natural radioactivity determination in samples of Peperomia pellucida commonly used as a medicinal herb. Journal of Environmental Radioactivity, 116(2013), 148-151.
Switala, J. & Loewen, P. C. (2002). Diversity of properties among catalases. Archives of Biochemistry and Biophysics, 401, 145-154.
Tadesse, G., Reneela, P., & Dekebo, A. (2012). Isolation and characterization of natural products from Helinus mystachnus (Rhamnaceae). Journal of Chemical and Pharmaceutical Research, 4(3), 1756-1762.
Talmale, S. A., Bhujade, A. M., & Patil, M. B. (2014). Phytochemical analysis of stem bark and root bark of Zizyphus Mauritiana. International Journal of Innovative Science Engineering and Technology, 1(4), 526-535.
Tan, M. C., Tan, C. P., & Ho, C. W. (2013). Effects of extraction solvent system, time and temperature on total phenolic content of henna (Lawsonia inermis) stems. International Food Research Journal, 20(6), 3117-3123.
Tawaha, K., Alali, F. Q., Gharaibeh, M., Mohammad, M., & El-Elimat, T. (2007). Antioxidant activity and total phenolic content of selected Jordanian plant species. Food Chemistry, 104, 1372-1378.
Thangavel, A., Balakrishnan, S., Arumugam, A., Duraisamy, S., & Muthusamy, S. (2014). Phytochemical screening, gas chromatography-mass spectrometry (GCMS) analysis of phytochemical constituents and anti-bacterial activity of Aerva lanata (L.) leaves. African Journal of Pharmacy and Pharmacology, 8(5), 126- 135.
Thorat, I. D., Jagtap, D. D., Mohapatra, D., Joshi, D. C., Sutar, R. F., & Kapdi, S. S. (2013). Antioxidants, their properties, uses in food products and their legal implications. International Journal of Food Studies. 2, 81-104.
Tiwari, P., Kumar, B., Kaur, M., Kaur, G., & Kaur, H. (2011). Phytochemical screening and extraction : A review. Internationale Pharmaceutica Sciencia, 1(1), 98-106.
Trenzado, C., Hidalgo, M. C., García-Gallego, M., Morales, A. E., Furné, M., Domezain, A., et al. (2006). Antioxidant enzymes and lipid peroxidation in sturgeon Acipenser naccarii and trout Oncorhynchus mykiss. A comparative study. Aquaculture, 254(1-4), 758-767.
Trifunschi, S. I. & Ardelean, D. G. (2013). Flavonoid extraction from Ficus carica leaves using different techniques and solvents. Zbornik Matice srpske za prirodne nauke, 81-86.
Tyagi, S., Singh, G., Sharma, A., & Aggarwal, G. (2010). Phytochemicals as candidate therapeutics: An overview. International Journal of Pharmaceutical Sciences Review and Research, 3(1), 53-55.
Urquiza-Martínez, M. V. & Navarro, B. F. (2016). Antioxidant capacity of food. Free Radical and Antioxidants, 6(1), 1-12.
Vaishali Rai, M., Pai, V. R., Pratapchandra Kedilaya, H., & Hedge, S. (2013). Preliminary phytochemical screening of members of Lamiaceae family: Leucas linifolia, Coleus aromaticus and Pogestemon patchouli. International Journal of Pharmaceutical Sciences Review and Research, 21(1), 131-137.
Vaanholt, L. M., Speakman, J. R., Garland Jr, T., Lobley, G. E., & Visser, G. H. (2008). Protein synthesis and antioxidant capacity in aging mice: effects of long-term voluntary exercise. Physiological and Biochemical Zoology, 81(2), 148-157.
Vergara-Rodríguez, D., Mathieu, G., Samain, M. S., Armenta-Montero, S., & Krömer, T. (2017). Diversity, distribution, and conservation status of Peperomia (Piperaceae) in the state of Veracruz, Mexico. Tropical Conservation Science, 10, 1-28.
Wadood, A., Ghufran, M., Jamal, S. B., Naeem, M., Khan, A., & Ghaffar, R., et al (2013). Phytochemical analysis of medicinal plants occurring in local area of Mardan. Biochemistry & Analytical Biochemistry, 2(4), 1-4.
Wan Afiqah Syahirah Wan Ghazali, A'attiyyah Ab Alim, Kannan, T. P., Nor Azah Mohd Ali, Nurul Asma Abdullah & Khairani Idah Mokhtar. (2016). Anticancer properties of Malaysian herbs: A review. Archives Of Orofacial Science, 11(2), 19-25.
Wang, M., Zhang, Z., & Wang, X. (2014). Superoxide dismutase 2 as a marker to differentiate tuberculous pleural effusions from malignant pleural effusions. Clinics, 69(12), 799-803.
Waty, D. R., Saputri, F. C., & Mun’im, A. (2017). Secondary metabolites screening and acute toxicity test of Peperomia pellucida (L.) Kunth methanolic extracts. International Journal of PharmTech Research, 10(1), 31-38.
Wei, L. S., Wee, W., Yong, J. F. S., & Syamsumir, D.S. (2011). Characterization of anticancer, antimicrobial, antioxidant properties and chemical compositions of Peperomia pellucida leaf extract. Acta Medica Iranica, 49(10), 670-674.
Weydert, C. J. & Cullen, J. J. (2010). Measurement of superoxide dismutase, catalase and glutathione peroxidase in cultured cells and tissue. Nature protocols, 5(1), 51-66.
Wickens, A. P. (2001). Ageing and the free radical theory. Respiration Physiology, 128, 379-391.
Wuerges, J., Lee, J. W., Yim, Y. I., Yim, H. S., Kang, S. O., & Carugo, K. D. (2004). Crystal structure of nickel-containing superoxide dismutase reveals another type of active site. Proceedings of the National Academy of Sciences of the United States of America, 101(23), 8569-8574.
Xiu, W. P., Akowuah, G. A., & Jin, H. C. (2013). Evaluation of the sub–acute oral toxic effect of methanol extract of Clinacanthus nutans leaves in rats. Journal of Acute Disease, 29-32.
Xu, S., Li, N., Ning, M. M., Zhou, C. H., Yang, Q. R., & Wang, M. W. (2006). Bioactive compounds from Peperomia pellucida. Journal of Natural Products, 69, 247-250.
Xu, J., Hu, Z. Q., Wang, C., Yin, Z. Q., Wei, Q., Zhou, L. J., et al (2014). Acute and subacute toxicity study of 1, 8-cineole in mice. International Journal of Clinical and Experimental Pathology, 7(4), 1495-1501.
Yadav, A., Kumari, R., Yadav, A., Mishra, J. P., Srivatva, S., & Prabha, S. (2016). Antioxidants and its functions in human body-A review. Research in Environment and Life Sciences, 9(11), 1328-1331.
Yadav, M., Chatterji, S., Gupta, S. K., & Watal, G. (2014). Preliminary phytochemical screening of six medicinal plants used in traditional medicine. International Journal of Pharmacy and Pharmaceutical Sciences, 6(5), 539-542.
Yamakoshi, J., Saito, M., Kataoka, S., & Kikuchi, M. (2002). Safety evaluation of proanthocyanidin-rich extract from grape seeds. Food and Chemical Toxicology, 40, 599-607.
Yashin, A., Yashin, Y., Xia, X., & Nemzer, B. (2017). Antioxidant activity of spices and their impact on human health: A review. Antioxidants, 6, 1-18.
Yusuf, A. Z., Zakir, A., Shemau, Z., Abdullahi, M., & Halima, S. A. (2014). Phytochemical analysis of the methanol leaves extract of Paullinia pinnata linn. Journal of Pharmacognosy and Phytotherapy, 6(2), 10-16.
Zhang, Q. (2015). Effects of extraction solvents on phytochemicals and antioxidant activities of walnut (Juglans. Regia L.) green husk extracts. European Journal of Food Science and. Technology, 3(5), 15-21.
?ivkovi?, I., Rajnpreht, I., Mini?, R., Miti?, K., Aleksi?, I., Kadri?, J., et al. (2016). Characterization of Intor: Swiss albino mice adopted in the Institute of Virology, Vaccines and Sera–Torlak, Belgrade in the early twentieth century. Acta Veterinaria, 66(3), 279-293.
Zulazeze Sahri, Sharifalillah Nordin & Haryani Harun (2012). Malaysia indigenous herbs knowledge representation. Paper presented at the Knowledge Management International Conference (KMICe), Johor Bahru, Malaysia. Retrieved from https://www.researchgate.net/publication/309038361_Malaysia_Indigenous_He rbs_Knowledge_Representation.
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