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Type :thesis
Subject :QK Botany
Main Author :Amirah Shaari
Title :Phytochemical screening,antioxidant capacity and toxicological study of Malaysian Peperomia pellucida (L.) Kunth
Place of Production :Tanjong Malim
Publisher :Fakulti Sains dan Matematik
Year of Publication :2019
Corporate Name :Universiti Pendidikan Sultan Idris
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Abstract : Universiti Pendidikan Sultan Idris
This study performed to investigate the chemical compounds, toxicology, in vitro and in  vivo  antioxidant  capacity  of  Malaysian  Peperomia  pellucida  plant  towards  ICR mice.   The  qualitative  chemical  compound  test  was  performed  and  the  antioxidant capacity of P.  pellucida plant extract was analyzed by 2,2-diphenyl-1-picrylhydrazyl (DPPH),  β-carotene   bleaching  and  oxygen  radical  absorbance  capacity  (ORAC) assays. The in vivo sub-acute  toxicity study that used the best solvent from  in vitro assay was done using ICR mice which were  randomly divided. The different doses of 50 mg/kg, 150 mg/kg, 200 mg/kg, 500 mg/kg and 1000 mg/kg  body weight of extract were administered daily for 21 days to the treated mice. The antioxidant  enzymes in liver and kidney of mice were investigated using standard assays.  P. pellucida only  exhibited the presence of alkaloid, phenol and saponin. The ability to scavenge free radical in  DPPH assay showed that methanol extract of Selangor (ground) sample depicted the highest value of  1108  ± 5.568 mg/ml compared  to the other samples.  In  β-carotene bleaching  assay,  the  ethyl   acetate  extract  of  Penang  (ground)  sample  offered  the highest  percentage  (86.111%)  of   scavenging  activity from  free  radical  compared  to the  other  samples.  In  ORAC  assay,   methanol  extract  of  Penang  (ground)  sample showed the highest value 397.691 μmol of Trolox  equivalent/μl. Findings showed that ethyl acetate and methanol extracts exhibited a good solvent  for antioxidant capacity of  P.  pellucida.  However,  in  sub-acute  toxicity  study,  ethyl   acetate  and  methanol extract of higher doses lead to mortality in mice. Furthermore,  the   administration of ethyl acetate and methanol extract of P. pellucida did not affect the antioxidant  enzymes in liver and kidney of mice. In conclusion, the antioxidant capacity of  P. pellucida can  only  be  seen  by  in  vitro  assay.  Thus,  P.  pellucida  could  be  a  potential  source  of antioxidant substance.  

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