UPSI Digital Repository (UDRep)
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Abstract : Universiti Pendidikan Sultan Idris |
The study aimed to investigate the chemical constituents from Polyalthia lateriflora,
Alphonsea elliptica and Alphonsea cylindrica as well as their cytotoxic, antioxidant and
xanthine oxidase inhibitory activities. These samples were collected from Peninsular
Malaysia and then extracted sequentially using hexane, dichloromethane and methanol.
The chemical compounds were isolated and purified by various chromatographic
techniques and their structures were elucidated via spectroscopic method especially
Nuclear Magnetic Resonance, Mass Spectrometry, Infrared, Ultraviolet and comparison
with literature. Cytotoxic activity was analysed on MCF-7 human breast cancer cells while
antioxidant activity was measured using 2,2-diphenyl-1-picrylhydrazyl (DPPH). Study on
P. lateriflora has yielded six compounds which were lupeol, stigmasterol, Omethylmoschotaline,
atherospermidine, lysicamine and liriodenine. Meanwhile, five
compounds namely kinabaline, atherospermidine, liriodenine, N-methylouregidione and
cepharadione A were obtained from the barks of A. elliptica. In addition, study on the barks
of A. cylindrica has found eleven compounds in which two are new namely iraqiine and
kareemine. Other compounds were identified as isoursuline, cyathocaline, kinabaline,
muniranine, isooncodine, atherospermidine, O-methylmoschatoline, Nmethylouregidione,
and stigmasterol. Antioxidant activity was tested on six compounds in
which iraqiine, muniranine and kinabaline showed the highest activity with IC50 values of
48.77, 44.51 and 64.28mg/ml, respectively. Thirteen compounds were tested against MCF-
7 human breast cancer cells of which isoursuline possessed the most potent inhibitory
activity with IC50 value of 33 μg/ml. Ten compounds displayed moderate xanthine oxidase
inhibitory activity. As a conclusion, 22 compounds were successfully isolated from three
plant species and two of them are new derivatives of oxoaporphine and
bisbenzylisoquinoline alkaloids. Some compounds have been identified as potentially
cytotoxic, antioxidant and xanthine oxidase inhibitor. The findings of this study can
enhance the understanding of the chemotaxonomy aspect and potential of the Annonaceae
family in traditional and modern medicine. |
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