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Type :thesis
Subject :QK Botany
Main Author :Obaid, Ahmed Kareem
Title :Secondary metabolites from three species of annonaceae plants and their biological activities
Place of Production :Tanjong Malim
Publisher :Fakulti Sains dan Matematik
Year of Publication :2019
Corporate Name :Universiti Pendidikan Sultan Idris
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Abstract : Universiti Pendidikan Sultan Idris
The study aimed to investigate the chemical constituents from Polyalthia lateriflora, Alphonsea elliptica and Alphonsea cylindrica as well as their cytotoxic, antioxidant and xanthine oxidase inhibitory activities. These samples were collected from Peninsular Malaysia and then extracted sequentially using hexane, dichloromethane and methanol. The chemical compounds were isolated and purified by various chromatographic techniques and their structures were elucidated via spectroscopic method especially Nuclear Magnetic Resonance, Mass Spectrometry, Infrared, Ultraviolet and comparison with literature. Cytotoxic activity was analysed on MCF-7 human breast cancer cells while antioxidant activity was measured using 2,2-diphenyl-1-picrylhydrazyl (DPPH). Study on P. lateriflora has yielded six compounds which were lupeol, stigmasterol, Omethylmoschotaline, atherospermidine, lysicamine and liriodenine. Meanwhile, five compounds namely kinabaline, atherospermidine, liriodenine, N-methylouregidione and cepharadione A were obtained from the barks of A. elliptica. In addition, study on the barks of A. cylindrica has found eleven compounds in which two are new namely iraqiine and kareemine. Other compounds were identified as isoursuline, cyathocaline, kinabaline, muniranine, isooncodine, atherospermidine, O-methylmoschatoline, Nmethylouregidione, and stigmasterol. Antioxidant activity was tested on six compounds in which iraqiine, muniranine and kinabaline showed the highest activity with IC50 values of 48.77, 44.51 and 64.28mg/ml, respectively. Thirteen compounds were tested against MCF- 7 human breast cancer cells of which isoursuline possessed the most potent inhibitory activity with IC50 value of 33 μg/ml. Ten compounds displayed moderate xanthine oxidase inhibitory activity. As a conclusion, 22 compounds were successfully isolated from three plant species and two of them are new derivatives of oxoaporphine and bisbenzylisoquinoline alkaloids. Some compounds have been identified as potentially cytotoxic, antioxidant and xanthine oxidase inhibitor. The findings of this study can enhance the understanding of the chemotaxonomy aspect and potential of the Annonaceae family in traditional and modern medicine.

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