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Callus formation and phytochemical constituents of finger eggplant (Solanum Melongena L.)

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General Information Cached
Title
Callus formation and phytochemical constituents of finger eggplant (Solanum Melongena L.)
Type
Article
Main Author
Norhayati Daud
Additional Authors
  • Norizzah Jaafar Sidek
  • Sinang, Som Cit
  • Nurul Fazira Omar
Subject
Q Science (General)
Language Code
eng
Bibliographic Details
Place of Production
Tanjong Malim
Publisher
Fakulti Sains dan Matematik
Year of publication
2020
Degree Type
Others
Corporate Name
Universiti Pendidikan Sultan Idris

Abstract

This study examined the efficiency of callus induction on optimum concentrations of NAA (αnaphthaleneacetic acid) and BAP (6-benzyladenine) from culturing stem and leaf explants of finger eggplant (Solanum sp.) and investigated the phytochemical constituents of callus tissue. Seeds were sterilized by using 3 and 5 % Clorox solution, which gave the highest number of survival seeds (100 %) and were grown in vitro plantlets. The highest frequency of callus induction (100.00 ± 0.00 %) was obtained from stems and leaf explants that were excised from in vitro plantlets. The stem explants cultured on MS medium consisted of 1.0 mg/L NAA + 1.0 mg/L BAP, giving the maximum mean callus fresh weight (0.14 ± 0.05 g). Meanwhile, the leaf explants cultured on MS medium consisted of 0.5 mg/L NAA + 2.0 mg/L BAP, generating the maximum mean callus fresh weight (0.48 ± 0.10 g). The highest frequency of callus induction (88.00 ± 1.60 %) was obtained in solidified MS medium supplemented with 0.5 mg/L NAA + 2.0 mg/L BAP, producing the maximum mean fresh weight of callus (1.54 ± 0.27 g) and dry weight (0.90 ± 0.01 g). The results of the Phytochemical screenings of callus and dried leaf extracts indicated the presence of alkaloids, flavonoids, terpenoids, and saponins.  

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