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Type :thesis
Subject :SB Plant culture
Main Author :Nurul Shifa Radzuan
Title :Morphogenesis studies and growth optimization of Carica papaya L. var Eksotika through tissue culture system
Place of Production :Tanjong Malim
Publisher :Fakulti Teknikal dan Vokasional
Year of Publication :2019
Corporate Name :Universiti Pendidikan Sultan Idris
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Abstract : Universiti Pendidikan Sultan Idris
The research aimed to study the morphogenesis and growth optimization of Carica papaya L. var Eksotika through tissue culture system. A study was conducted to test the effect of 16 different concentrations and combinations of Benzylaminopurine (BAP) and Napthalene-Acetic Acid (NAA) on three different types of Carica papaya L. var Eksotika explants, namely leaf, stem and root. Complete plant regeneration was successfully achieved when root, stem and leaf aseptic explants were cultured on MS medium supplemented with various combinations of BAP and NAA. MS medium supplemented with 2.0 mg/L BAP was found to be the optimum medium and stem explants was the most responsive explants producing 3.800 ± 0.323 shoots per explants. MS media supplemented with 0.5 mg/L BAP and 2.0 mg/L NAA was most optimum for root induction. Optimum callus induction was obtained when root explants were culture on MS medium fortified with 2.0 mg/L BAP + 1.0 mg/L NAA. Meanwhile, synthetic seeds of Carica papaya L. were produced when microshoots were encapsulated with 4.0% sodium alginate solution added with 2.0 mg/L BAP. Finally, acclimatization of Carica papaya L. was accomplished when plantlets were transferred to the soil. The research showed that in vitro propagation of Carica papaya L. through tissue culture system was successfully achieved. In conclusion, the regeneration process, callus induction, synthetic seed, microscopic studies and acclimatization of Carica papaya L. var Eksotika were achieved. Micropropagation had been successfully obtained from the explants culture on combination and concentration of BAP and NAA. This research has provided positive implications towards the development of agriculture industry especially in Malaysia and also worldwide.

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