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Abstract : Universiti Pendidikan Sultan Idris |
The research aimed to study the morphogenesis and growth optimization of Carica
papaya L. var Eksotika through tissue culture system. A study was conducted to test
the effect of 16 different concentrations and combinations of Benzylaminopurine
(BAP) and Napthalene-Acetic Acid (NAA) on three different types of Carica papaya
L. var Eksotika explants, namely leaf, stem and root. Complete plant regeneration was
successfully achieved when root, stem and leaf aseptic explants were cultured on MS
medium supplemented with various combinations of BAP and NAA. MS medium
supplemented with 2.0 mg/L BAP was found to be the optimum medium and stem
explants was the most responsive explants producing 3.800 ± 0.323 shoots per
explants. MS media supplemented with 0.5 mg/L BAP and 2.0 mg/L NAA was most
optimum for root induction. Optimum callus induction was obtained when root
explants were culture on MS medium fortified with 2.0 mg/L BAP + 1.0 mg/L NAA.
Meanwhile, synthetic seeds of Carica papaya L. were produced when microshoots
were encapsulated with 4.0% sodium alginate solution added with 2.0 mg/L BAP.
Finally, acclimatization of Carica papaya L. was accomplished when plantlets were
transferred to the soil. The research showed that in vitro propagation of Carica
papaya L. through tissue culture system was successfully achieved. In conclusion, the
regeneration process, callus induction, synthetic seed, microscopic studies and
acclimatization of Carica papaya L. var Eksotika were achieved. Micropropagation
had been successfully obtained from the explants culture on combination and
concentration of BAP and NAA. This research has provided positive implications
towards the development of agriculture industry especially in Malaysia and also
worldwide. |
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