The efficacy of hibiscus rosa-sinensis leaf extracts against candida spp. causing candidiasis

S. Zuhaira

Abstract

Over the years, the World Health Organization (WHO) has recommended traditional medicines as safe remedies for ailments of both microbial and non-microbial origins. The use of herbals has increased worldwide in recent years, as they are believed to be safer than modern medicines with lesser or no side effects. The present study has been designed to determine the role of Hibiscus rosa-sinensis leaves extract in-vitro antifungal activities against Candida albicans, Candida tropicalis, and Candida glabrata species. The leaves of H. rosa-sinensis were soaked in methanol and ethanol for solvent extraction. The extraction yield was diluted in DMSO (99.9%) to obtain 6 different concentrations (1000, 500, 250, 125, 62.5 and 31.25 mg/ml), and was then impregnated on blank discs. The disc diffusion method was used to test against 3 different candida species for sensitivity test. Basic phytochemical constituent’s detection on phenol, carbohydrate, tannins and saponins were done on H. rosasinensis. The extract that shows largest zone of inhibition of candida species is the leaf extract with ethanol. 1000 mg/ml of the leaf extract shows the highest inhibition zone against C. albicans, C. tropicalis and C. glabrata which are 9 mm, 8 mm and 9 mm respectively. Carbohydrate test showed both extractions highly positive and ethanol extract exhibited the highest saponin compared with methanol extract. It was found that ethanol extract of H. rosasinensis showed more potent anti-candidiasis compared with methanol extract.

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