UPSI Digital Repository (UDRep)
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Abstract : Universiti Pendidikan Sultan Idris |
Ficus carica L. is one of Moraceae family which natively to Westerns Asia and
distributed worldwide and economically important especially in sericulture, horticulture, food and
cosmetic industries. Ficus carica L. contain numerous of nutrients, minerals and vitamins. This
plant also produces secondary metabolites, latex cells. The richness of nutrients and
latex cells make this plant possessed many pharmacological effects which is importantly to
medicinal and drugs industries. The demand for this plant is very high despite the
production of fig is very inconsistent from year to year. So, this study aims to establish and
determine the optimum concentration of benzylaminopurine (BAP) and napthelene acetic acid
(NAA) for Ficus carica L. through plant tissue culture system by following completely randomized
design. The shoots were able to be regenerated from stems explants at most optimum concentration
of 1.0 mg/L BAP. For callus induction, all stem, shoot, petiole, and root explants able to emerge
calli when cultured in MS medium with optimum combinations of BAP and NAA; 1.0 mg/L BAP +
0.5 mg/L NAA, 0.5 mg/L BAP + 1.0 mg/L NAA, 1.5 mg/L BAP + 1.0 mg/L NAA, and 1.0 mg/L BAP + 1.0
mg/L NAA respectively. The explants also were tested with other plant growth regulators such as
thidiazuron (TDZ), Kinetin, indole-3-butyric acid (IBA), and 2,4-
dichlorophenoxyacetic acid (2,4-D) for a comparison. The results still showed that BAP and NAA were
the best plant growth regulators used compared to others combinations. The micromorphology study
shows the presence of trichomes on adaxial and abaxial of in vitro and in vivo leaves. Stoma was
also visible on all surfaces except on adaxial of in vivo leaf. The acclimatization process allowed
the plantlets survived in garden soil with 86.67% after weeks 8 cultured. Lastly, synthetic seeds
of Ficus carica L. were produced with stem explants that encapsulated in 4% of sodium
alginate supplemented with 1.0 mg/L BAP. In conclusion, this study was successively established
an alternative way in propagating Ficus carica L. through plant tissue culture system. The
implication of this study would able to help in give a good information to public and improving
agricultural
sector with new technologies.
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