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Type :thesis
Subject :Q Science
Main Author :Fadhlul Khaliq Ab Patah
Title :Effects of growth regulators on Plant Tissue Culture of Ficus carica L.
Place of Production :Tanjong Malim
Publisher :Fakulti Teknikal dan Vokasional
Year of Publication :2020
Corporate Name :Universiti Pendidikan Sultan Idris
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Abstract : Universiti Pendidikan Sultan Idris
Ficus  carica  L.  is  one  of  Moraceae  family  which  natively  to  Westerns  Asia  and distributed worldwide and economically important especially in sericulture, horticulture, food and  cosmetic industries. Ficus carica L. contain numerous of nutrients, minerals and vitamins.  This   plant  also  produces  secondary  metabolites,  latex  cells.  The  richness  of nutrients and  latex cells make this plant possessed many pharmacological effects which is importantly  to   medicinal  and  drugs  industries.  The  demand  for  this  plant  is  very  high despite the  production of fig is very inconsistent from year to year.  So, this study aims to establish  and   determine  the  optimum  concentration  of  benzylaminopurine  (BAP)  and napthelene acetic acid  (NAA) for Ficus carica L. through plant tissue culture system by following completely randomized  design.   The shoots were able to be regenerated from stems explants at most optimum concentration  of 1.0 mg/L BAP. For callus induction, all stem, shoot, petiole, and root explants able to emerge  calli when cultured in MS medium with  optimum  combinations  of  BAP  and  NAA;  1.0  mg/L  BAP  +   0.5  mg/L  NAA,  0.5 mg/L BAP + 1.0 mg/L NAA, 1.5 mg/L BAP + 1.0 mg/L NAA, and 1.0 mg/L BAP + 1.0  mg/L NAA respectively. The explants also were tested with other plant growth regulators such    as     thidiazuron    (TDZ),    Kinetin,    indole-3-butyric    acid    (IBA),    and    2,4-  dichlorophenoxyacetic acid (2,4-D) for a comparison. The results still showed that BAP and NAA were  the best plant growth regulators used compared to others combinations. The micromorphology study  shows the presence of trichomes on adaxial and abaxial of in vitro and in vivo leaves. Stoma was  also visible on all surfaces except on adaxial of in vivo leaf. The acclimatization process allowed  the plantlets survived in garden soil with 86.67% after weeks 8 cultured. Lastly, synthetic seeds  of Ficus carica L. were produced with  stem  explants  that  encapsulated  in  4%  of  sodium   alginate  supplemented  with  1.0 mg/L BAP. In conclusion, this study was successively established  an alternative way in propagating Ficus carica L. through plant tissue culture system. The  implication of this study would able to help in give a good information to public and improving  agricultural sector with new technologies.  

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