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Type :thesis
Subject :QD Chemistry
Main Author :Muhammad Ammar Mohd Azhar
Title :Phytochemistry and antioxidant activity of selected species from lauraceae family
Place of Production :Tanjong Malim
Publisher :Fakulti Sains dan Matematik
Year of Publication :2021
Corporate Name :Universiti Pendidikan Sultan Idris
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Abstract : Universiti Pendidikan Sultan Idris
The purposes of the study were to analyse the essential oils composition, phytochemicals, and antioxidant activity of selected species of the genus Litsea (L. costalis, L. machilifolia and L. globularia), Beilschmiedia (B. kunstleri, B. insignis and B. pahangensis) and Cryptocarya (C. impressa, C. infectoria and C. rugulosa) from Lauraceae family. The essential oils were obtained via hydrodistillation technique and their chemical compositions were determined by gas chromatography (GC-FID) and gas chromatography-mass spectrometry (GC-MS). The phytochemicals were obtained using chromatography techniques and their structures were confirmed by spectroscopic analysis and comparison with literature. The antioxidant activity of the essential oils and extracts was determined using DPPH free radical scavenging assay. The study showed that the major components of L. costalis, L. machilifolia and L. globularia essential oils were β-caryophyllene (12.6%), β-sesquiphellandrene (29.5%) and β-caryophyllene (25.2%), respectively. Besides, α-longipinene (11.0%), (E)-nerolidol (32.4%) and δ-cadinene were the major components from the essential oils of B. kunstleri, B. insignis and B. pahangensis, respectively. As for C. impressa, C. infectoria and C. rugulosa, the essential oils consisted of α-cadinol (40.7%), β- caryophyllene (25.4%) and bicyclogermacrene (15.6%), respectively. The dichloromethane and methanol extracts of B. insignis yielded seven phytochemicals identified as vanillic acid, vanillin, benzyl benzoate, benzoic acid, betulin, β-sitosterol and β-sitostenone. All essential oils displayed weak activity in DPPH radical scavenging assay, while the L. costalis methanolic extract showed the strongest activity (IC50 21.9 ppm) comparable to ascorbic acid (IC50 2.9 ppm). In conclusion, the major composition of essential oils was sesquiterpene hydrocarbons, meanwhile carboxylic acids and terpenes were successfully isolated from B. insignis extracts. The implication of the study showed the essential oils and extracts of the genus Litsea, Beilschmiedia and Cryptocarya have potential in pharmaceutical applications.

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