UPSI Digital Repository (UDRep)
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Abstract : Universiti Pendidikan Sultan Idris |
Experiment were carried out to establish the most optimum culture condition and to
identify the most responsive explants for regeneration Brassica oleraceae L. var
Capitata through plant tissue culture system. Acclimatization process for established
plantlets was also conducted. The experimental design of this study was Complete
Randomized Design (CRD) method. For shoot regeneration using different
concentrations of BAP and NAA, the most responsive explant was stem explants in
which MS medium supplemented with 1.5 mg/L BAP and 0.5 mg/L NAA produced
8.400 ± 0.889 shoots per explant. Whereas, the combination auxin and cytokinin also
showed stem explants was the most responsive. Subsequently, for root regeneration
using different concentrations of BAP and NAA, the most responsive explant was
leave in which MS media supplemented with 0.5 mg/L BAP and 0.5 mg/L NAA
produced 51.133 ± 2.929 roots per explant. While the combination of auxin and
cytokinin showed the most responsive was root. Next, for callus induction with
combination of BAP and NAA showed that petiole was the most responsive explant.
Callus was white in colour with friable structure. Meanwhile, for callus induction with
combination of auxin and cytokinin showed stem was the most responsive. Callus was
light brown and friable. In addition, synthetic seeds were produced when microshoots
were encapsulated with 4.0% sodium alginate solution added with 1.0 mg/L BAP and
0.5 mg/L NAA. The germination rate was 6.832 ± 0.622 shoots per explant. Finally,
the acclimatization of this plantlet succeeded with combination of (black soil and red
soil at 2:1 ratio) with the survival rate of 86.67%. This research showed that
morphogenesis studies of Brassica oleraceae L. var Capitata through tissue culture
system was successfully achieved. Further studies on chemical such as secondary
metabolites contained in the callus and explants extract should be carried out. |
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