UPSI Digital Repository (UDRep)
Start | FAQ | About

QR Code Link :

Type :thesis
Subject :Q Science
Main Author :Noor Fara'Ain Daud
Title :Morphogenesis studies of Brassica oleraceae L.var Capitata through tissue culture system
Place of Production :Tanjong Malim
Publisher :Fakulti Teknikal dan Vokasional
Year of Publication :2021
Corporate Name :Universiti Pendidikan Sultan Idris
PDF Guest :Click to view PDF file
PDF Full Text :Login required to access this item.

Abstract : Universiti Pendidikan Sultan Idris
Experiment were carried out to establish the most optimum culture condition and to identify the most responsive explants for regeneration Brassica oleraceae L. var Capitata through plant tissue culture system. Acclimatization process for established plantlets was also conducted. The experimental design of this study was Complete Randomized Design (CRD) method. For shoot regeneration using different concentrations of BAP and NAA, the most responsive explant was stem explants in which MS medium supplemented with 1.5 mg/L BAP and 0.5 mg/L NAA produced 8.400 ± 0.889 shoots per explant. Whereas, the combination auxin and cytokinin also showed stem explants was the most responsive. Subsequently, for root regeneration using different concentrations of BAP and NAA, the most responsive explant was leave in which MS media supplemented with 0.5 mg/L BAP and 0.5 mg/L NAA produced 51.133 ± 2.929 roots per explant. While the combination of auxin and cytokinin showed the most responsive was root. Next, for callus induction with combination of BAP and NAA showed that petiole was the most responsive explant. Callus was white in colour with friable structure. Meanwhile, for callus induction with combination of auxin and cytokinin showed stem was the most responsive. Callus was light brown and friable. In addition, synthetic seeds were produced when microshoots were encapsulated with 4.0% sodium alginate solution added with 1.0 mg/L BAP and 0.5 mg/L NAA. The germination rate was 6.832 ± 0.622 shoots per explant. Finally, the acclimatization of this plantlet succeeded with combination of (black soil and red soil at 2:1 ratio) with the survival rate of 86.67%. This research showed that morphogenesis studies of Brassica oleraceae L. var Capitata through tissue culture system was successfully achieved. Further studies on chemical such as secondary metabolites contained in the callus and explants extract should be carried out.

References

Amir, H.M., Shamsudin, M.N., Hussein, M.A., & Radam, A. (2007). An Economic

Evaluation of IPM Practices in Cabbage Production in Cameron Highlands,

Pahang. Economic and Technology Management Review. Vol. 2, 11-21.

 

Babbar, S.B., Agarwal, P.K., Sahay, S. & Bhojwani, S.S. (2004). Isolated Microspore

Culture of Brassica : An Experimental Tool for Developmental Studies and Crop

Improvement. Indian Journal of Biotechnology, Vol. 3, 185-202.

 

Bapat, V.A. (2000). Synthetic seeds: a novel concept in seed biotechnology. Retrieved

from http://www.barc.gov.in/publications/nl/index.html ( accessed on 18 January

2019 )

 

Chandra, S., Bandopadhyay, R., Kumar, V. & Chandra, R. (2010). Acclimatization of

tissue cultured plantlets : from laboratory to land. Biotechnol Lett. 32,1199-1205.

https://doi: 10.1007/s10529-010-0290-0

 

Chauffour, F., Bailly, M., Perreau, F., Cueff, G., Suzuki, H., & Collet, B. (2019).

Multi-omics Analysis Reveals Sequential Roles for ABA during Seed Maturation.

Retrieved from http://www.plantphysiol.org/content/180/2/1198 ( accessed on 18

January 2019 )

 

Chooi, H.O. (2008). Vegetables for Health & Healing. Kuala Lumpur: Utusan

Publications and Distributors Sdn. Bhd, pp: 58-59.

 

Cristea, T.O. (2013). The Influence of pH on Microspore Embryogenesis of White

Cabbage (Brassica oleraceae L.). Not Sci Biol, 5 (4) : 485-489.

 

Cseke, L.J., Kaufman, P. B., Podila, G.K., & Chung J.T (2004). Handbook of

Molecular and Cellular Methods in Biology and Medicine, Second Ed. New York:

CRC Press

 

Dixon, R.A., & Gonzales, R.A. (1994). Plant Cell Culture: A Practical Approach 2nd

Edition. Oxford University Press. Oxford.

 

Dixon, R.G. (2007). Vegetables Brassicas and Related Crucifers. United Kingdom:

CABI Head Office Nosworthy Way.

 

El-Esawi, M.A. (2018). Brassica Germplasm : Characterization, Breeding and

Utilization : British Library Cataloguing, pp : 1-2

 

Evans, D.E., Coleman, J. & Keavns, A. (2003). Plant Cell Culture. London & New

York: BIOS Scientific Publishers, pp : 7-8

 

Fowke, L.C. (1995). Transmission and Scanning Electron Microscopy for Plant

Protoplasts, Cultured Cells and Tissues. New York ; Springer, Berlin Heidelberg.

 

Frusciante, L., Barone, A., Carputo, D., Ereolano, M.R., Della Rocca, F., & Esposito,

S. (2000). Evaluation and Use of Plant Biodiversity for Food and Pharmaceutical.

Fitoterpia, 71 : 66-S72.

 

Gana, A.S. (2010). The Role Of Synthetic Growth Hormones In Crop Multiplication

And Improvement. African Journal of Biotechnology Vol. 10(51), pp. 10330-

10334.

 

Hajam, M.A., Hassan, G.I., Bhat, T.A., Bhat, I.A., Rather, A.M., Parray, E.A., et al

(2017). Understanding Plant Growth Regulators, Their Interplay : for Nursery

Establishment in Fruits. International Journal of Chemical Studies, 5(5), 905-910.

 

Hamid, A.U. (2018). A Beginners‟ Guide to Scanning Electron Microscopy. Center

for Engineering Research. https://doi:org/10.1007/978-3-319-98482-7

 

Hussein, N. (2012). Effects of Nutrient Media Constituents on Growth and

Development of Banana (Musa spp.) Shoot Tips Cultured in Vitro. African

Journal of Biotechnology. 11(37), pp. 9001-9006

 

Ibrahim, M.A. (2015, 26 March). FAMA‟S Helping Hand for Cabbage Growers,

BERNAMA.

 

Ichsan, M. (2018). In vitro regeneration and acclimatization of Carica papaya L.

Retrieved from

http://www.malrep.uum.edu.my/rep/Record/oai:ir.upsi.edu.my:3811 ( accessed on

20 March 2019 )

 

Ikeuchi, M., Sugimoto, K. & Iwase, A. (2013). Plant Callus : Mechanisms of

Induction and Repression. The Plant Cell, 25(9), 3159-3173.

https://doi:10.1105/tpc. 113.116053

 

ISAAA (2004). Tissue Culture Technology. Retrieved from http://www.isaaa.org/kc.

(accessed on 24 March 2019)

 

Jamilah Syafawati Yaacob., Noraini Mahmad., Rosna Mat Taha., Normadiha

Mohamed., Anis Idayu Mad Yussof., & Azani Saleh. (2014). Optimization of

Culture Conditions (Sucrose, pH, and Photoperiod) for In Vitro Regeneration and

Early Detection of Somaclonal Variation in Ginger Lime (Citrus assamensis).

https://doi:10.1155/2014/262710

 

Jusoff, K. (2009). Estimating Cabbage Production in Cameron Highlands, Malaysia

Using IKONOS Data. Canadian Research & Development Center of Sciences and

Cultures, Vol. 2 (2).

 

Kadhimi, A. A., Alhasnawi, A. N., Isahak, A., Ashraf, M. F., Mohamad, A., Doni, F.,

et al (2014). Use of biotechnology to improve the tolerance in rice (Oryza sativa)

to drought stress. Journal of Pure and Applied Microbiology, 8(5), pp : 4001-

4010.

 

Kamada, H., Kobayashi, K., Kiyosue, T., & Harada, H. (1989). Stress induced

somatic embryogenesis in carrot and its application to synthetic seed production.

In Vitro Cellular & Development Biology. 25(12), pp : 1163-1166.

 

Lohry, R. (2007). Micronutrients : Functions, Sources and Application Methods.

Retrieved from https://www.agry.purdue.edu/CCA/2007/2007/Proceedings

(accessed on 24 March 2019)

 

Marschner, P. (2012). Marschner’s Mineral Nutrition of Higher Plants (Third

Edition) : School of Agriculture, Food and Wine, The University of Adelaide,

Australia, p.p : 135-189

 

Mastuti, R., Munawarti, A. & Firdiana, E.R. (2017). The Combination Effect of

Auxin and Cytokinin on in vitro Callus Formation of Physalis angulate L. – A

Medicinal Plant. International Conference on Global Resources Conservation;

https://doi:org/10.1063/ 1.5012721

 

McDonald, M.R., Warland, J., & McKeown, A.W. (2006). Long-Term Climate And

Weather Patterns In Relation To Crop Yield: A Minireview. Botany, Vol 84 (7).

 

Min, B. C., Omar, K., & Lin, O. C. (2006). 1001 Garden Plants in Singapore.

Singapore : National Parks Publication.

 

Muhammad, Z.A., Iqbal, H., Sohaib R., Muhammad A.Z., Muhammad, S.Z., Zaheer,

A., & Sabir H.S. (2012). In vitro Response of Cytokinin and Auxin to Multiple

Shoot Regeneration in Solanum tuberosum L. American-Eurasian J. Agric. &

Environ. Sci., 12 (11): 1522-1526.

 

Mumo, N.N., Rimberia, F.K., Mamati, G.E., & Kihurani, A.W. (2013). In

vitro regeneration of selected Kenyan papaya (Carica papaya L.)

lines through shoot tip culture. African Journal of Biotechnology, Vol 12(49),

6826-6832

 

Murashige, T. & Skoog, F.A (1962). Revised Medium for Rapid Growth and Bio

Assays with Tobacco Tissue Cultures. Physiologia Plantarum. 15,pp: 473-497.

 

New World Encyclopedia, retrieved from

https://www.newworldencyclopedia.org/entry/Cabbage, ( accessed on 21 March

2017 )

 

Ngomuo, M., Mneney, E., & Ndakidemi, P. (2013). The Effects of Auxins and

Cytokinin on Growth and Development of (Musa sp.) Var. “Yangambi” Explants

in Tissue Culture. American Journal of Plant Sciences, 4(11), 2174

 

Ningappa, K. (2016). Evaluation of Cabbage (Brassica oleraceae L. var. capitata)

Genotypes for Compactness and Internal Tipburn Resistance Under Rain Shelter.

Department of Olericulture College of Horticulture Kerala Agricultural

University Vellanikkara, Thrissur-680 656 Kerala, India.

 

North, J., Ndakidemi, P., & Laubscher, C.P. (2010). The Potential of Developing an

in vitro Method for Propagating Strelitziaceae. African Journal Biotechnology.

9(45), pp. 7583-7588.

 

Panjaitan, S. B., Aziz, M. A., Rashid, A. A. and Saleh. N. M. (2007). In-Vitro Plantlet

Regeneration from Shoot Tip of Field-grown Hermaphrodite Papaya (Carica

papaya L. cv. Eksotika). International Journal of Agriculture and Biology. 9 (6),

827-832.

 

Pavlovic, S., Adzic, S., Cvikic, D., Zdravkovic, J., & Zdravkovic, M. (2012). In Vitro

Culture as a Part of Brassica oleraceae var. capitata L. Breeding. GENETIKA,

44(3) pp : 611-618.

 

Prakash, S. & Hoque, M.I. (2002). Culture Media and Containers. In: International

Atomic Energy Agency (ed.): Low Cost Options for Tissue Culture Technology in

Developing Countries. Proceedings of a technical meeting, 26-30 August,

Vienna, Austria.

 

Ranabhatt, H. & Kapor, R. (2017). Plant Biotechnology. Woodhead Publishing India,

pp : 4

 

Ravanfar, S.A., Aziz, M.A., Rashid, A.A., & Salim, S. (2014). In Vitro Adventitious

Shoot Regeneration from Cotyledon Explant of Brassica oleraceae subsp. Italic

& Brassica oleraceae subsp. Capitata using TDZ and NAA. Pak. J. Bot., 46(1):

329-335.

 

Rishla, K., Sithara, A., & Deepa, P. (2017). Effect of NaCl stress on callus induction

in centella asiatica (l.) urban. European Journal of Biomedical and

Pharmaceutical Sciences, 4 (9).

 

Roberta, H.S. (2000). Plant Tissue Culture Techniques and Experiments. California:

Academic Press, pp : 54-60

 

Rosna M.T. (2004). Kultur Tisu Tumbuhan Berbunga : Penerbit Universiti Malaya, pp

: 23

 

Rout, G.R. & Sahoo, S. (2015). Role of Iron in Plant Growth and Metabolism.

Reviews in Agricultural Science, 3: 1-24.

 

Saad, A.I.M. & Elshahed, A.M. (2012). Plant Tissue Culture Media : Department of

Botany and Microbiology, Faculty of Science, Sebha University, Libya, pp : 29-

39.

 

Safaryani, N., Haryanti, S., & Hastuti, E. (2007). Pengaruh Suhu dan Lama

Penyimpanan Terhadap Penurunan Kadar Vitamin C Brokoli (Brassica oleracea

L). Sellula, 15(2).

 

Saikia, M., Shrivastava, K., & Sureshkumar, S. (2013). Effect of culture media and

growth hormones on callus induction in Aquallaria malaccensis Lam., a

medicinally and commercially important tree species of north east india. Asia

Journal of Biological Sciences,6(2), 96-105.

 

Samir, C.D., & Usha, A. (2020). In Vitro Propagation Strategies of Medicinally

Important Berry Crop, Lingonberry (Vaccinium vitis-idaea L.). Agronomy 2020,

Vol 10, 744; https://doi:10.3390/agronomy10050744

 

Shamsudin, M.N., Amir, H.M. and Radam, A. (2010). Economic Benefits of

Sustainable Agricultural Production : The Case of Integrated Pest Management in

Cabbage Production. EnvironmentAsia 3 (special issue) 168-174.

 

Sharma, A., Lal, D., & Sutradhar, M. (2017). Effect of growth regulators on callus

morphology of rice anther culture. Journal of Applied Biology & Biotechnology,

Vol 5 (3), 068-071.

 

Shekhawat, M., & Manokari, M. (2017). Foliar Micromorphology of in Vitro -

Cultured Shoots and Field-Grown Plants of Passiflora Foetida. Horticultural Plant

Journal, Vol 3. https://doi:10.1016/j.hpj.2017.01.009.

 

Shekhawat, M., Manokari, M., & Kannan, N. (2017). Micromorphological response

towards altered environmental conditions in subsequent stages of in vitro

propagation of Morinda coreia. Environmental and Experimental Biology. Vol

15.https://doi:10.22364/eeb.15.06.

 

Siong, P.K., Mohajer, S. & Taha, R.M. (2012). Production of artificial seeds derived

from encapsulated in vitro micro shoots of cauliflower, Brassica oleracea var.

botrytis. Romanian Biotechnological Letter. 17(4), 7549-7556

 

Siong, P.K., Mohajer, S., Taha, R.M. (2012). Production of artificial seeds derived

from encapsulated in vitro micro shoots of cauliflower, Brassica oleraceae var.

botrytis. Romanian Biotechnological Letters, 17(4), 7549-7556.

 

Smith, H.R. (2000). Plant Tissue Culture Techniques and Experiments.California:

Academic Press.

 

Su, Y.H., Liu, Y.B, & Zhang, X.S. (2011). Auxin–Cytokinin Interaction Regulates

Meristem Development. Molecular Plant. https://doi:10.1093/mp/ssr007

 

Sutter, E.G., Shackel, K., & Diaz, J.C. (1992). Acclimatization of tissue cultured

plants. Acta Horticulturae 314. Propagation of Ornamental Plants, 115-119.

 

Swapp, S. (2019). What is Scanning Electron Microscopy (SEM). Retrieved from

https://serc.carleton.edu/research_education/geochemsheets/techniques/SEM.html

( accessed on 20 March 2019 )

 

Teo, C.H., & Seow, C.C. (1992). A Pulsed NMR Method for the Study of Starch

Retrogradation. Starch, Vol. 44 (8), 288-292

 

Tilaar, W. Sumeru, A., Bagyo, Y., Jeany, P., & Francien, H.T (2012). Shoot Induction

from Broccoli Explant Hypocotyls and Biosynthesis of Sulforaphane.

International Journal of Basic & Applied Sciences, Vol 12 (6), 44-48.

 

Touchell, D., Smith, J. and Ranney, T.G. (2008). Novel Applications of Plant Tissue

Culture : North Carolina State University, North Carolina, pp : 22-23

 

Tripathi, D.K., Singh, V.P., Chauhan, D.K., Prasad, S.M., and Dubey, N.K. (2014).

Improvement of Crops in the Era of Climatic Changes : Banaras Hindu

University, Varanasi, India, pp : 197-216

 

Volenikova, M. & Ticha, I. (2001). Insertion profiles in stomatal density and sizes in

Nicotiana tabacum L. plantlets. Biology plantarum. 44(2): 161-165

 

Wang, K.L.C., Li, H., & Ecker, J.R. (2002). Ethylene Biosynthesis and Signaling

Networks. American Society of Plant Biologists. https://doi:org/10.1105

/tpc.001768

 

Welsh, J.R. (1991). Dasar-dasar genetika dan pemuliaan tanaman. Jakarta: Penerbit

Erlangga.

 

White, R.P. (1997). Handbook of Plant Tissue Culture. New Delhi: Publications and

Information Division Indian Council of Agricultural Research, pp : 25

 

Wiart, C. (2002). Medicinal Plants of Southeast Asia. Petaling Jaya, Selangor:

Prentice Hall Pearson, pp: 111.

 

Willis, K.J. (ed.) ( 2017). State of the World’s Plants 2017. Report. Royal Botanic

Gardens, Kew.

 

Yaacob, J.S., Saleh, A., Elias, H., Abdullah, S., Mahmad, N. & Mohamed, N. (2014).

In Vitro Regeneration and Acclimatization Protocols of Slected Ornamental Plants

(Agapanthus praecox, Justice betonica and Celosia cristata). Sains Malaysiana,

43(5) : 715-722.

 

Yaacob, J.S., Mahmud,N., Taha, R.M., Mohamed, N., Yussof, A.I.M., & Saleh, A.

(2014). Optimization of culture conditions (sucrose, pH, and photoperiod) for in

vitro regeneration and early detection of somaclonal variation in ginger lime

(citrus assamensis). The Scientific World Journal, articles ID 262710.

 

Yasmin, S., Nasiruddin, K.M., Begum, R., & Talukder, S.K. (2003). Regeneration

and establishment of potato plantlets through callus formation with BAP and

NAA. Asian Journal of Plant Sciences, 2(12). 936-940.

 

Yusnita. (2004). Kultur jaringan, cara memperbanyak tanaman secara efisien.

Jakarta: AgroMedia Pustaka.

 

Yussof, A.I.M., Wafa, S.N. & Taha, R.M. (2012). Plant regeneration and synthetic

seeds production of Brassica oleraceae var. italic. International Symposium on

Sustainable Vegetable Production in Southeast Asia.

https://doi:10.17660/ActaHortic.2012.958.21

 

Zazimalova, E., Petrasek, J., & Benkova, E. (2014). Auxin and Its Role in Plant

Development. London : Springer Wien HeidelbergNew York Dordrecht London,

pp : 171

 

Zheng, Y., Xu, M., Shen, R., Qiu, S., & Ouyang, Z. (2013). Effects of experimental

warming on stomatal traits in leaves of maize (Zea may L.). Ecology and

Evolution. 3(9) : 3095-3111. https://doi:10.1002/ece3.674.

 

Znidarcic, D., Valic, N., & Trdan, S. (2008). Epicuticular Wax Content in the Leaves

of Cabbage (Brassica oleraceae L.var Capitata) as a Mechanical Barrier against

Three Insect Pests. Acta Agriculturae Slovenica, 1-2.

 


This material may be protected under Copyright Act which governs the making of photocopies or reproductions of copyrighted materials.
You may use the digitized material for private study, scholarship, or research.

Back to previous page

Installed and configured by Bahagian Automasi, Perpustakaan Tuanku Bainun, Universiti Pendidikan Sultan Idris
If you have enquiries with this repository, kindly contact us at pustakasys@upsi.edu.my or Whatsapp +60163630263 (Office hours only)