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Type :thesis
Subject :Q Science
Main Author :Noor Fara\'Ain Daud
Title :Morphogenesis studies of Brassica oleraceae L.var Capitata through tissue culture system
Place of Production :Tanjong Malim
Publisher :Fakulti Teknikal dan Vokasional
Year of Publication :2021
Corporate Name :Universiti Pendidikan Sultan Idris
PDF Guest :Click to view PDF file

Abstract : Universiti Pendidikan Sultan Idris
Experiment were carried out to establish the most optimum culture condition and to identify the most responsive explants for regeneration Brassica oleraceae L. var Capitata through plant tissue culture system. Acclimatization process for established plantlets was also conducted. The experimental design of this study was Complete Randomized Design (CRD) method. For shoot regeneration using different concentrations of BAP and NAA, the most responsive explant was stem explants in which MS medium supplemented with 1.5 mg/L BAP and 0.5 mg/L NAA produced 8.400 ± 0.889 shoots per explant. Whereas, the combination auxin and cytokinin also showed stem explants was the most responsive. Subsequently, for root regeneration using different concentrations of BAP and NAA, the most responsive explant was leave in which MS media supplemented with 0.5 mg/L BAP and 0.5 mg/L NAA produced 51.133 ± 2.929 roots per explant. While the combination of auxin and cytokinin showed the most responsive was root. Next, for callus induction with combination of BAP and NAA showed that petiole was the most responsive explant. Callus was white in colour with friable structure. Meanwhile, for callus induction with combination of auxin and cytokinin showed stem was the most responsive. Callus was light brown and friable. In addition, synthetic seeds were produced when microshoots were encapsulated with 4.0% sodium alginate solution added with 1.0 mg/L BAP and 0.5 mg/L NAA. The germination rate was 6.832 ± 0.622 shoots per explant. Finally, the acclimatization of this plantlet succeeded with combination of (black soil and red soil at 2:1 ratio) with the survival rate of 86.67%. This research showed that morphogenesis studies of Brassica oleraceae L. var Capitata through tissue culture system was successfully achieved. Further studies on chemical such as secondary metabolites contained in the callus and explants extract should be carried out.

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