|
UPSI Digital Repository (UDRep)
|
|
| ||||||||||||||||||||||||||
| Abstract : Universiti Pendidikan Sultan Idris |
| This study was conducted to synthesize and characterize silver nanoparticles (AgNPs) using a rapid green synthesis approach. Antibacterial properties of AgNPs were evaluated. Extract of Melia Dubia (Neem) and collagen produced from the fish scales were used as reducing and stabilizer agents respectively. Uv-Vis Spectroscopy, Scanning Electron Microscopy (SEM), Energy-Dispersive X-Ray Spectroscopy (EDX), Fourier-Transform Infrared Spectroscopy (FTIR), and Dynamic Light Scattering (DLS) were used to characterize the synthesized AgNPs. Evaluation of the synthesized AgNP's antibacterial activity was conducted, with Staphylococcus Aureus (S. aureus) as Gram-positive and Escherichia Coli (E. coli) as the negative bacteria. The peak of absorbance for the synthesized AgNPs was at 454 nm, indicating conformed AgNPs. SEM image showed semi-evenly distributed rod shapes. The EDX data revealed presence of the metallic silver. Meanwhile, FTIR analysis indicated presence of C2H2, C=O, N-H groups. DLS showed an average size of 437.6 nm. XRD showed calculation for particle size using d = K?/?.cos?. Average size of AgNPs was 141.81 � 5 nm. AgNPs also displayed tangible antibacterial activity towards the S. aureus and pathogenic E. coli. AgNPS were successfully synthesized and evaluated for their antibacterial properties. The outcomes being the multifaceted biological activities alongside application of biocompatible green nanoparticles, is discoverable in the field of Nanomedicine. ? 2021. All Rights Reserved. |
| References |
Ahmed, S., Ahmad, M., Swami, B. L., & Ikram, S. (2016). A review on plants extract mediated synthesis of silver nanoparticles for antimicrobial applications: A green expertise. Journal of Advanced Research, 7(1), 17-28. doi:10.1016/j.jare.2015.02.007 Ahmed, S., Ullah, S., Ahmad, M., Swami, B. L., & Ikram, S. (2015). Green synthesis of silver nanoparticles using azadirachta indica aqueous leaf extract. J.Radiat.Res.Appl.Sci., 9(1), 1-7. Retrieved from www.scopus.com Ajitha, B., Kumar Reddy, Y. A., Reddy, P. S., Jeon, H. -., & Ahn, C. W. (2016). Role of capping agents in controlling silver nanoparticles size, antibacterial activity and potential application as optical hydrogen peroxide sensor. RSC Advances, 6(42), 36171-36179. doi:10.1039/c6ra03766f Anandalakshmi, K., Venugobal, J., & Ramasamy, V. (2016). Characterization of silver nanoparticles by green synthesis method using pedalium murex leaf extract and their antibacterial activity. Applied Nanoscience (Switzerland), 6(3), 399-408. doi:10.1007/s13204-015-0449-z Beyene, H. D., Werkneh, A. A., Bezabh, H. K., & Ambaye, T. G. (2017). Synthesis paradigm and applications of silver nanoparticles (AgNPs), a review. Sustainable Materials and Technologies, 13, 18-23. doi:10.1016/j.susmat.2017.08.001 Chowdhury, S., Yusof, F., Sulaiman, N., & Faruck, M. O. (2017). The aggregation study and characterization of silver nanoparticles doi:10.4028/www.scientific.net/SSP.263.165 Retrieved from www.scopus.com Dehnavi, A. S., Raisi, A., & Aroujalian, A. (2013). Control size and stability of colloidal silver nanoparticles with antibacterial activity prepared by a green synthesis method. Synthesis and Reactivity in Inorganic, Metal-Organic and Nano-Metal Chemistry, 43(5), 543-551. doi:10.1080/15533174.2012.741182 Govindarajan, M., Kadaikunnan, S., Alharbi, N. S., & Benelli, G. (2017). Single-step biological fabrication of colloidal silver nanoparticles using hugonia mystax: Larvicidal potential against zika virus, dengue, and malaria vector mosquitoes. Artificial Cells, Nanomedicine and Biotechnology, 45(7), 1317-1325. doi:10.1080/21691401.2016.1228664 Hamidi, A., Taghavizadeh Yazdi, M. E., Amiri, M. S., Hosseini, H. A., & Darroudi, M. (2019). Biological synthesis of silver nanoparticles in tribulus terrestris L. extract and evaluation of their photocatalyst, antibacterial, and cytotoxicity effects. Research on Chemical Intermediates, 45(5), 2915-2925. doi:10.1007/s11164-019-03770-y Joseph, S., & Mathew, B. (2015). Facile synthesis of silver nanoparticles and their application in dye degradation. Materials Science and Engineering B: Solid-State Materials for Advanced Technology, 195, 90-97. doi:10.1016/j.mseb.2015.02.007 Jyoti, K., Baunthiyal, M., & Singh, A. (2016). Characterization of silver nanoparticles synthesized using urtica dioica linn. leaves and their synergistic effects with antibiotics. J.Radiat.Res.Appl.Sci., 9(3), 217-227. Retrieved from www.scopus.com Kathiravan, V., Ravi, S., & Ashokkumar, S. (2014). Synthesis of silver nanoparticles from melia dubia leaf extract and their in vitro anticancer activity. Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, 130, 116-121. doi:10.1016/j.saa.2014.03.107 Liu, Y., Kim, S., Kim, Y. J., Perumalsamy, H., Lee, S., Hwang, E., & Yi, T. -. (2019). Green synthesis of gold nanoparticles using euphrasia officinalis leaf extract to inhibit lipopolysaccharide-induced inflammation through NF-κB and JAK/STAT pathways in RAW 264.7 macrophages. International Journal of Nanomedicine, 14, 2945-2959. doi:10.2147/IJN.S199781 Mudhafar, M., & Alsailawi, H. A. (2019). An expression study profile of proinflammatory cytokines in asthma patient. Journal of Asian Scientific Research, 9(12), 227-234. Retrieved from www.scopus.com Mudhafar, M., & Zainol, I. (2019). Medical values, antimicrobial, and anti fungal activities of polyalthia genus. International Journal of Pharmaceutical Research, 11(1), 90-96. Retrieved from www.scopus.com Mudhafar, M., Zainol, I., Alsailawi, H., & Aiza Jaafar, C. (2021). Synthesis and characterization of fish scales of hydroxyapatite/collagen-silver nanoparticles composites for the applications of bone filler. J Korean Ceramic Soc, 12, 1-1. Retrieved from www.scopus.com Mudhafar, M., Zainol, I., & Desa, S. (2019). Che nor aziza jaafar. "review of phytochemistry for. Polyalithia, 14, 119-147. Retrieved from www.scopus.com Mudhafar, M., Zainol, I., Jaafar, C. N. A., Alsailawi, H. A., & Desa, S. (2021). A review study on synthesis methods of agnanoparticles, considering antibacterial property and cytotoxicity. International Journal of Drug Delivery Technology, 11(2), 635-648. doi:10.25258/ijddt.11.2.72 Mudhafar, M., Zainol, I., Jaafar, C. N. A., Alsailawi, H. A., Majhool, A. A., & Alsaady, M. M. B. (2020). Phytochemical screening and characterization of meliadubia leaves extract for antimicrobial activity against escherichia coli staphylococcus and aureus. Indian Journal of Ecology, 47(2), 493-496. Retrieved from www.scopus.com Ojo, O. A., Oyinloye, B. E., Ojo, A. B., Afolabi, O. B., Peters, O. A., Olaiya, O., . . . Osunlana, O. (2017). Green synthesis of silver nanoparticles (AgNPs) using talinum triangulare (jacq.) willd. leaf extract and monitoring their antimicrobial activity. Journal of Bionanoscience, 11(4), 292-296. doi:10.1166/jbns.2017.1452 Pak, Z. H., Karimi, N., & Abbaspour, H. (2017). Effects of silver nanoparticle exposure on growth, physiological and biochemical parameters of dracocephalum moldavica L. Iranian Journal of Plant Physiology, 7(4), 2173-2183. doi:10.22034/ijpp.2017.537982 Pugazhendhi, A., Prabakar, D., Jacob, J. M., Karuppusamy, I., & Saratale, R. G. (2018). Synthesis and characterization of silver nanoparticles using gelidium amansii and its antimicrobial property against various pathogenic bacteria. Microbial Pathogenesis, 114, 41-45. doi:10.1016/j.micpath.2017.11.013 Rathod, D., Golinska, P., Wypij, M., Dahm, H., & Rai, M. (2016). A new report of nocardiopsis valliformis strain OT1 from alkaline lonar crater of india and its use in synthesis of silver nanoparticles with special reference to evaluation of antibacterial activity and cytotoxicity. Medical Microbiology and Immunology, 205(5), 435-447. doi:10.1007/s00430-016-0462-1 Sawadkar, P., Sibbons, P., Ahmed, T., Bozec, L., & Mudera, V. (2019). Engineering of a functional tendon using collagen as a natural polymer. ACS Biomaterials Science and Engineering, doi:10.1021/acsbiomaterials.8b01544 Shaik, M. R., Khan, M., Kuniyil, M., Al-Warthan, A., Alkhathlan, H. Z., Siddiqui, M. R. H., . . . Adil, S. F. (2018). Plant-extract-assisted green synthesis of silver nanoparticles using origanum vulgare L. extract and their microbicidal activities. Sustainability (Switzerland), 10(4) doi:10.3390/su10040913 Singh, P., Kim, Y. J., Wang, C., Mathiyalagan, R., & Yang, D. C. (2016). Weissella oryzae DC6-facilitated green synthesis of silver nanoparticles and their antimicrobial potential. Artificial Cells, Nanomedicine and Biotechnology, 44(6), 1569-1575. doi:10.3109/21691401.2015.1064937 Sportelli, M. C., Clemente, M., Izzi, M., Volpe, A., Ancona, A., Picca, R. A., . . . Cioffi, N. (2018). Exceptionally stable silver nanoparticles synthesized by laser ablation in alcoholic organic solvent. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 559, 148-158. doi:10.1016/j.colsurfa.2018.09.046 Sripriya, R., & Kumar, R. (2015). A novel enzymatic method for preparation and characterization of collagen film from swim bladder of fish rohu (labeo rohita). Food and Nutrition Sciences, 6(15), 1468-1478. Retrieved from www.scopus.com Sundarrajan, P., & Shetty, S. (2019). Synthesis and characterization of silver nanoparticles using various plant extracts and their various. Environmental Applications, 5, 91. Retrieved from www.scopus.com Tarannum, A., Muvva, C., Mehta, A., Rao, J. R., & Fathima, N. N. (2016). Phosphonium based ionic liquids-stabilizing or destabilizing agents for collagen? RSC Advances, 6(5), 4022-4033. doi:10.1039/c5ra22441a |
| This material may be protected under Copyright Act which governs the making of photocopies or reproductions of copyrighted materials. You may use the digitized material for private study, scholarship, or research. |