UPSI Digital Repository (UDRep)
|
UPSI Digital Repository (UDRep)
|
|
|
| ||||||||||||||||||||||||||
| Abstract : Universiti Pendidikan Sultan Idris |
| This research aimed to examine the possible effectiveness of treatment with a freshly formulated new probiotic versus Phaleria macrocarpa leaves extract indicated by the histopathological changes occurred to the colon of the immunocompromised New Zealand White (NZW) rabbits. The study included 40 rabbits, divided into 4 groups. Three of the groups were fed with the immunosuppressive medication (azathioprine, 500 mg/kg/day) to be immunocompromised, Group B was treated with P. macrocarpa extract, Group Ctreated with our developed probiotic, and the group D left untreated as a positive control, Group A regarded as control negative. The duration of immunocompromising drug induction was set as 14 days; and the period of treatment was set for another 14 days. Distinctly, the treated rabbits were showing improvements of healing processes compared to non-treated, where the signs of lack of normal mucous folds, different degrees of mucosal edema and congestion, submucosal hemorrhage, accumulative score of gross colon morphology and major severe lesions of the colon tissue of the non-treated were significantly improved in the probiotic treated, as well as, P. macrocarpa treated groups. As a conclusion, the results indicated that uptake of the formulated probiotic could strengthen rabbits' immune responses of the immunoco-mpromised rabbits significantly (both the specific and non-specific responses) and retain the weakened colon tissues (caused by the immunosuppressant drug). These findings are recommended to be used in future for development of promising commercial supplements to mitigate the medical risks that comes with immunosuppressant medications currently given to animals or human patients under certain circumstances. ? Published under licence by IOP Publishing Ltd. |
| References |
(2001). Health and Nutritional Properties of Probiotics in Food Including Powder Milk with Live Lactic Acid Bacteria, Retrieved from www.scopus.com Al-Safi, S., Tashtoush, B., & Hassan, M. (2003). Comparison of the effects azathioprine and its novel non-mercaptopurine analog on antibody response in rabbits. Polish Journal of Pharmacology, 55(2), 239-243. Retrieved from www.scopus.com Altaf, R., Asmawi, M. Z. B., Dewa, A., Sadikun, A., & Umar, M. I. (2013). Phytochemistry and medicinal properties of phaleria macrocarpa (scheff.) boerl. extracts. Pharmacognosy Reviews, 7(13), 73-80. doi:10.4103/0973-7847.112853 Azam, R., Ghafouri-Fard, S., Tabrizi, M., Modarressi, M. -., Ebrahimzadeh-Vesal, R., Daneshvar, M., . . . Motevaseli, E. (2014). Lactobacillus acidophilus and lactobacillus crispatus culture supernatants downregulate expression of cancer-testis genes in the MDA-MB-231 cell line. Asian Pacific Journal of Cancer Prevention, 15(10), 4255-4259. doi:10.7314/APJCP.2014.15.10.4255 Chen, Q. -., Duan, W. -., Chen, G. -., & Han, G. -. (2011). Review and expectation of application of the mechanism of the biological immune system in work safety management. Paper presented at the Procedia Engineering, , 11 18-26. doi:10.1016/j.proeng.2011.04.621 Retrieved from www.scopus.com Coman, M. M., Verdenelli, M. C., Cecchini, C., Silvi, S., Orpianesi, C., Boyko, N., & Cresci, A. (2014). In vitro evaluation of antimicrobial activity of lactobacillus rhamnosus IMC 501®, lactobacillus paracasei IMC 502® and SYNBIO® against pathogens. Journal of Applied Microbiology, 117(2), 518-527. doi:10.1111/jam.12544 Copeland, D. R., McVay, M. R., Dassinger, M. S., Jackson, R. J., & Smith, S. D. (2009). Probiotic fortified diet reduces bacterial colonization and translocation in a long-term neonatal rabbit model. Journal of Pediatric Surgery, 44(6), 1061-1064. doi:10.1016/j.jpedsurg.2009.02.014 Cremonini, F., Mullan, B. P., Camilleri, M., Burton, D. D., & Rank, M. R. (2002). Performance characteristics of scintigraphic transit measurements for studies of experimental therapies. Alimentary Pharmacology and Therapeutics, 16(10), 1781-1790. doi:10.1046/j.1365-2036.2002.01344.x Garcia, N. (2015). Benefits of Phaleria Macrocarpa for Reduce Blood Sugar Levels, Reduce Blood Clotting, Retrieved from www.scopus.com Grizzle, W. E., Fredenburgh, J. L., & Myers, R. B. (2008). Fixation of tissues. Theory and Practice of Histological Techniques, , 53-74. Retrieved from www.scopus.com Mistry, V. V., & Kosikowski, F. V. (1985). Growth of lactic acid bacteria in highly concentrated ultrafiltered skim milk potentates. Journal of Dairy Science, 68(10), 2536-2543. doi:10.3168/jds.S0022-0302(85)81134-6 Shetty, N. (2005). Immunology: Introductory textbook. Immunology: Introductory Textbook, Retrieved from www.scopus.com Taherian-Esfahani, Z., Abedin-Do, A., Nouri, Z., Mirfakhraie, R., Ghafouri-Fard, S., & Motevaseli, E. (2016). Lactobacilli differentially modulate mTOR and wnt/ βcatenin pathways in different cancer cell lines. International Journal of Cancer Management, 9(3), 1-7. doi:10.17795/ijcp-5369 Weinshilboum, R. (2001). Thiopurine pharmacogenetics: Clinical and molecular studies of thiopurine methyltransferase. Drug Metabolism and Disposition, 29(4 II), 601-605. Retrieved from www.scopus.com Zabihollahi, R., Motevaseli, E., Sadat, S. M., Azizi-Saraji, A. R., Asaadi-Dalaie, S., & Modarressi, M. H. (2012). Inhibition of HIV and HSV infection by vaginal lactobacilli in vitro and in vivo. DARU, Journal of Pharmaceutical Sciences, 20(1) doi:10.1186/2008-2231-20-53 Zhang, M., Wang, F., Jiang, L., Liu, R., Zhang, L., Lei, X., . . . Ren, F. (2013). Lactobacillus salivarius REN inhibits rat oral cancer induced by 4-nitroquioline 1-oxide. Cancer Prevention Research, 6(7), 686-694. doi:10.1158/1940-6207.CAPR-12-0427 |
| This material may be protected under Copyright Act which governs the making of photocopies or reproductions of copyrighted materials. You may use the digitized material for private study, scholarship, or research. |