Association between time of day differences in muscle force production and the post-translational modification (Phosphorylation) of muscle proteins

Zulezwan Ab Malik

Abstract

The maximum force output of skeletal muscle exhibits a circadian rhythm and is associated with time-of-day differences in athletic performance. We investigated whether the diurnal difference in force output is associated with changes to the post-translational state of muscle proteins using non-targeted proteomic techniques. Ten physically active men (mean ± SD; age 26.7 ± 3.7 y, height 1.77 ± 0.08 m, body mass 74.9 ± 9.8 kg) performed experimental sessions in the morning (0800 h) and evening (1700 h) that were counterbalanced in order of administration and separated by at least 72 h. Each session included measurements of maximal voluntary contraction (MVC; including twitch-interpolation) and peak rate of force development (RFD) of the knee extensors. Samples of vastus lateralis were collected immediately after exercise and were analysed by ‘top-down’ and ‘bottom-up’ proteomic methods. Muscle force in the evening (754.4 ± 112.9 N) was significantly (P

References

Aargard, P., Simonsen, E.B., Andersen, J. L., Magnusson, P. & Dyhre-Poulsen, P. (2002).

Increased rate of force development and neural drive of human skeletal muscle following

resistance training. J Appl Physiol., 93, 1318-1326.

Ackermann, T. F., Boini, K. M., Beier, N., Scholz, W., Fuchss, T., & Lang, F. (2011).

EMD638683, a novel SGK inhibitor with antihypertensive potency. Cellular Physiology

and Biochemistry, 28, 137-146.

Boateng, S. Y., & Goldspink, P. H. (2008). Assembly and maintenance of the sarcomere night

and day. Cardiovasc Res, 77(4): 667-675.

Burniston, J. G., & Hoffman, E. P. (2011). Proteomic responses of skeletal and cardiac muscle to

exercise. Expert Rev Proteomics, 8, 361-377.

Gresham, K. S., and Stelzer, J. E. (2016). The contributions of MyBP-C and TnI phosphorylation

to beta-adrenergic enhancement of in vivo cardiac function. J. Physiol. 594, 669–686

Larsson, L., & Moss, R. L. (2005). Maximum velocity of shortening in relation to myosin

isoform composition in single fibres from human skeletal muscles. The Journal of

Physiology, 472, 595-614.

McLeod M., Breen L., Hamilton D.L., Philp A. (2016). Live strong and prosper: the importance

of skeletal muscle strength for healthy ageing. Biogerontology. 17(3): 497–510.

Podobed, P, Pyle, WG, Ackloo, S, Alibhai, FJ, Tsimakouridze, EV, Ratcliffe, WF, Mackay, A,

Simpson, J, Wright, DC, Kirby, GM. (2014). The day/night proteome in the murine heart.

Am J Physiol Regul Integr Comp Physiol 307:R121-R137.

Reilly, T. & Waterhouse, J. (2009). Sports performance: is there evidence that the body clock

plays a role? European Journal of Applied Physiology, 106, 321-332.

Robertson, K. I., Gallagher, F.L., Goss, J.M., Jakicic, S.M. et al. (2018). Perceived exertion,

electromyography, and blood lactate during acute bouts of resistance exercise. Medicine

& Science in Sports & Exercise. 34, 3, 552–559,