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Type :thesis
Subject :QR Microbiology
Main Author :Alsailawi, Hasan Ali Jasim
Title :Allergenic characterization and biochemical stability of allergens in purple mud crab Scylla tranquabarica
Place of Production :Tanjong Malim
Publisher :Fakulti Sains dan Matematik
Year of Publication :2020
Notes :with cd
Corporate Name :Universiti Pendidikan Sultan Idris
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Abstract : Universiti Pendidikan Sultan Idris
  This study aims to characterize the allergenic properties and determine the biochemical stability of allergens of purple mud crab, Scylla tranquebarica. Raw extracts were  prepared from the muscle tissues of both mixed and specific body parts. Various treated extracts  were prepared from the muscles of mixed body parts. Protein profiles, allergenic proteins and  cross-reactive allergens were then identified by sodium  dodecyl  sulfate-polyacrylamide  gel   electrophoresis  (SDS-PAGE), immunoblotting and immunoblotting inhibition test techniques,  respectively, using sera from crab-allergic patients. The identification of major allergens were  analyzed by mass spectrometry. This study showed that raw mixed part extract contains the most  protein bands and allergenic proteins than the specific body parts and all treated extracts. Five  major allergens were identified at 38, 42, 50, 63 and 73 kDa. Mass spectrometry analysis identified  the 42 and 50 kDa as arginine kinase, while the 38, 63 and 73 kDa were identified as tropomyosin,  actin and hemocyanin, respectively. The allergen extracts stored at -80 °C for all storage periods  of 3, 6, 9 and 12 months were indicated to have the same quality as the fresh raw mixed parts  extract. Immunoblotting inhibition tests showed the present of cross-reactivity among major and  minor allergens of crab, with either complete or partial inhibitions. Among the specific crab  parts, the female legs was found as the most allergenic parts, while the male abdomen was indicated  as the least allergenic. The high pressure steamed and white vinegar treated crabs were found to  have the lowest degree of stability and allergenicity among the thermal and non-thermal treated  crabs, respectively. As a conclusion, the allergenicity of S. tranquebarica varies depending on the  crab parts, processing treatments and storage conditions applied. Tropomyosin, arginine kinase,  actin and hemocyanin are identified as the major and cross-reactive allergens in this crab   species.  Tropomyosin  is  indicated  as  the  most  stable  allergen  of  S. tranquebarica. The  implication of this study is to provide insights into local mud crab allergens to improve strategy  of diagnosis, management, therapeutic and food manufacturing for crab allergic patients in this country.  

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