UPSI Digital Repository (UDRep)
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Abstract : Universiti Pendidikan Sultan Idris |
This study aims to characterize the allergenic properties and determine the
biochemical stability of allergens of purple mud crab, Scylla tranquebarica. Raw extracts were
prepared from the muscle tissues of both mixed and specific body parts. Various treated extracts
were prepared from the muscles of mixed body parts. Protein profiles, allergenic proteins and
cross-reactive allergens were then identified by sodium dodecyl sulfate-polyacrylamide gel
electrophoresis (SDS-PAGE), immunoblotting and immunoblotting inhibition test techniques,
respectively, using sera from crab-allergic patients. The identification of major allergens were
analyzed by mass spectrometry. This study showed that raw mixed part extract contains the most
protein bands and allergenic proteins than the specific body parts and all treated extracts. Five
major allergens were identified at 38, 42, 50, 63 and 73 kDa. Mass spectrometry analysis identified
the 42 and 50 kDa as arginine kinase, while the 38, 63 and 73 kDa were identified as tropomyosin,
actin and hemocyanin, respectively. The allergen extracts stored at -80 °C for all storage periods
of 3, 6, 9 and 12 months were indicated to have the same quality as the fresh raw mixed parts
extract. Immunoblotting inhibition tests showed the present of cross-reactivity among major and
minor allergens of crab, with either complete or partial inhibitions. Among the specific crab
parts, the female legs was found as the most allergenic parts, while the male abdomen was indicated
as the least allergenic. The high pressure steamed and white vinegar treated crabs were found to
have the lowest degree of stability and allergenicity among the thermal and non-thermal treated
crabs, respectively. As a conclusion, the allergenicity of S. tranquebarica varies depending on the
crab parts, processing treatments and storage conditions applied. Tropomyosin, arginine kinase,
actin and hemocyanin are identified as the major and cross-reactive allergens in this crab
species. Tropomyosin is indicated as the most stable allergen of S. tranquebarica. The
implication of this study is to provide insights into local mud crab allergens to improve strategy
of diagnosis, management, therapeutic and food
manufacturing for crab allergic patients in this country.
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