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Type :thesis
Subject :QD Chemistry
Main Author :Ahmad Azrul Hakim Yahaya
Title :Studies on essential oils, phytochemicals and antioxidant activity of Magnolia candollii H.Keng and Magnolia alba DC.(MAGNOLIACEAE)
Place of Production :Tanjong Malim
Publisher :Fakulti Sains dan Matematik
Year of Publication :2022
Corporate Name :Universiti Pendidikan Sultan Idris
PDF Guest :Click to view PDF file

Abstract : Universiti Pendidikan Sultan Idris
The purpose of this study was to investigate the essential oils, phytochemicals, and antioxidant activity of Magnolia candollii and Magnolia alba (Magnoliaceae). The essential oils were obtained via hydrodistillation technique and their chemical compositions were determined by gas chromatography-flame ionization detection (GC-FID) and gas chromatography-mass spectrometry (GC-MS). The phytochemicals were isolated using chromatography techniques and their structures were confirmed by spectroscopic data and comparison with literature. The antioxidant activity was determined through total phenolic content and DPPH free radical scavenging assay. The study showed that the essential oil of M. candollii consists of α-pinene (29.7%), elemol (10.2%), β-pinene (8.5%), β-caryophyllene (7.2%), α-terpineol (7.0%), and guaiol (5.4%). In addition, the major components of essential oil from M. alba were linalool (65.4%), β-caryophyllene (6.7%), and (E)-nerolidol (5.2%). Isolation and purification of M. candollii bark and M. alba leaf extracts yielded seven phytochemicals identified as licarin A, squalene, parthenolide, vanillin, stigmasterol acetate, friedelin, and β-sitosterol. Besides, linalool was successfully isolated from M. alba leaf essential oil. The methanol extract of M. candollii showed the highest antioxidant activity with phenolic content value as 380.0 mg GAE/g and 77.2% inhibition in the DPPH free radical scavenging assay. Licarin A demonstrated the highest antioxidant activity among the isolated compounds with percentage inhibition of 72.0% in the DPPH assay. In conclusion, the essential oils of M. candollii bark and M. alba leaf contain oxygenated monoterpenes and monoterpene hydrocarbons as principal components, respectively. Meanwhile, the phytochemical study of both plant extracts led to identification of different classes of natural compounds including neolignan, sesquiterpene lactone, phenolic, and terpenes. The study has significant implication on natural product research regarding essential oils and phytochemicals with promising antioxidant activity as well as their applications in the pharmaceutical industries.

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