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Type :thesis
Subject :Q Science
Main Author :Wong, Jun Xian
Title :Investigation of antimicrobial, antioxidant and toxicity analyses of Centella asiatica as potential fresh-cut produces preservative
Place of Production :Tanjong Malim
Publisher :Fakulti Sains dan Matematik
Year of Publication :2022
Corporate Name :Universiti Pendidikan Sultan Idris
PDF Guest :Click to view PDF file

Abstract : Universiti Pendidikan Sultan Idris
Investigation of antimicrobial, antioxidant and toxicity analyses of Centella asiatica as potential fresh-cut produces preservative were conducted. The aims of this study are antimicrobial and antioxidant activities of C. asiatica extracts; to determine the toxicity study of C. asiatica extract using the brine shrimp lethality assay (BSLA) and to determine the effectiveness of C. asiatica extract as a potential natural food additive on different types of fresh-cut fruits and vegetables (FCFV). The antimicrobial, antioxidant and toxicity assessments of C. asiatica extracts were then assayed based on the solvents (ethanol, methanol, water and hexane) and investigating of total phenolic content (TPC) and total flavonoid content (TFC). Results of objective-1 showed ethanolic extract recorded the highest extraction yield (14.7%), TPC (69.54 mg GAE/g) and TFC (13.90 mg QE/g). The ethanolic extract also exhibited the highest readings in disc diffusion assay (13.50 mm inhibition zone), minimum inhibition concentration (MIC; 7.81-125 mg/mℓ), minimum bactericidal concentration (MBC; 5.63-62.50 mg/mℓ), and minimum fungicidal concentration (MFC; 7.81-250 mg/mℓ). Objective-2 result showed the ethanolic extract recorded the highest inhibition concentration (IC50: 68.12 μg/mℓ). Based on BSLA, the result of objective-3 indicated that ethanolic extract exhibited non-toxic properties with LC50 of 597.72 mg/mℓ. The effectiveness of the ethanolic extract in inhibition of microbial growth and browning of FCFV was evaluated under room temperature displayed (RTD) (4 h, 22 ± 1℃) and chilled storage (CS) (4 days, 4 ± 0.5℃). Objective-4 results showed the range of microbial population reduction were 4.44–7.87 log CFU/g (RTD-FCFV) and 3.00–4.92 log CFU/g (CS-FCFV), respectively. Browning-indexes were ranged 4.72–9.26 (RTDFCFV) and 3.96–16.39 (CS-FCFV), respectively. In conclusion, the results had answered all objectives and successful. As an implication, the ethanolic extract is having potential to become alternative candidate in synthetic preservative.

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