The essential oil of Litsea resinosa Blume (Lauraceae) was obtained by hydrodistillation and was fully characterized by gas chromatography (GC-FID) and gas chromatography-mass spectrometry (GC-MS). A total of twenty-eight components were identified in the essential oil, which made up 90.8% of the total oil. The essential oil consists mainly of β-caryophyllene (22.5%), α-humulene (15.4%), caryophyllene oxide (9.2%), germacrene D (5.6%), and β-selinene (5.0%). The essential oil showed significant activity against acetylcholinesterase with a percentage inhibition of 72.4%. Keywords: Litsea resinosa, Lauraceae, essential oil, caryophyllene, acetylcholinesterase..

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