UPSI Digital Repository (UDRep)
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Total records found : 1 |
Simplified search suggestions : Noor Aiwa Rosman |
1 | 2017 article | Effects of adiponectin on markers of endothelial activation and markers of inflammation in human coronary artery endothelial cells Noor Aiwa Rosman This study investigates the effect of adiponectin on endothelial activation and inflammatory marker secretion by human coronary artery endothelial cells (HCEAC) in vitro. Methodology: HCAEC at the seventh passage were divided into two groups and incubated for 24 hours at 37° C and 5% CO2 as follows: Control, and adiponectin-treated (30 µg/ml adiponectin) groups. Supernatants were analysed for ICAM-1, E-selectin, PAI-1 and IL-6 and COX-2 using ELISA. RT-PCR was used to analyse gene expression of ICAM-1, E-selectin, PAI-, IL-6, COX-2, NFkBp50 and NFkBp65. Data were analysed using independent t-test.Results: ICAM-1 and E-selectin level was significantly higher in leptin-adiponectin-treated groups (P.. 460 hits |