AbstractThe purpose of this study was to investigate essential oils content, phytochemicals, computational modelling and bioactivities of four Knema species (Myristicaceae). Essential oils were extracted via hydrodistillation and were analyzed using gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). Phytochemicals were isolated using silica gel column chromatography and their chemical structures were elucidated via spectroscopic methods including nuclear magnetic resonance, infrared spectroscopy, mass spectrometry, and ultraviolet-visible spectroscopy. Computational studies were performed to assess the binding of isolated compounds to acetylcholinesterase (AChE) and 5-lipoxygenase (5-LOX), and their antibiofilm activities. The major components of the four Knema essential oils were as following: K. intermedia (_-muurolol, 20.1%), K. malayana (_-cadinene, 20.2%), K. hookeriana (_-caryophyllene, 26.2%), and K. furfuracea (bicyclogermacrene, 23.1%). Phytochemical investigati.....