The purpose of this research was to examine morphogenesis of Asparagus officinalis L. through tissue culture system. The experimental design used in this study was Completely Randomized Design (CRD) and 30 samples were used in each treatment.  Complete plant regeneration was successfully achieved when stem and root aseptic explants was cultured on MS medium supplemented with various combinations of plant growth regulators. MS medium supplemented with 2.0 mg/L BAP was found to be the optimum medium and stem explant was the most responsive explant producing 6.233 ± 0.810 shoots per explant. MS medium supplemented with 1.5 mg/L NAA was most optimum for root induction with 10.567 ± 0.841 roots per explant. Optimum callus induction was obtained when root explants were culture on MS medium fortified with 1.0 mg/L BAP and 1.0 mg/L NAA. Friable cream callus was produced. Meanwhile, the study with other auxin and cytokinin also showed the complete plant regeneration.  MS medium supplemented.....

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