The objective of this study is to extract, isolate, and elucidate the indole alkaloids from the roots of Kopsia singapurensis Ridl. collected from Kluang, Johor. The extraction process of the plant was carried out using hexane followed by dichloromethane (DCM) to yield hexane and DCM crude extracts. The isolation and purification process were performed by various chromatographic techniques namely column chromatography (CC), thin layer chromatography (TLC) and preparative thin layer chromatography (PTLC). The structures of isolated compounds were elucidated by using spectroscopic methods such as 1D-NMR (1H, 13C, and DEPT 135?) and 2D-NMR (COSY, HMQC, and HMBC), ultraviolet (UV), infrared (IR) and mass spectrometry (MS), and also by comparison with previous works. This study has successfully isolated eight indole alkaloids from the roots of K. singapurensis Ridl., tetrahydroalstonine, melodinine E, kopsifine, rhazinicine, kopsamine N(4)-oxide, aspidodasycarpine, kopsamine, and akuammidin.....

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