Papaya is one of the fruit crops cultivated in Malaysia and contributed to more than USD 2 million in gross production value in the year 2022 which also threatened by plant diseases. Recently, Fusarium equiseti has been observed to cause post-harvest papaya disease in Malaysia. To date, there is limited study on the molecular processes related to the papaya defense mechanism during fungal infection. The objectives of the study are to identify the causal agent causing postharvest disease in Papaya fruit and changes in protein abundance involved in the papaya_s defense mechanism. Firstly, a fungal growth pattern was observed after incubating the samples on Potato Dextrose Agar (PDA), giving evidence of Fusarium involvement in the postharvest disease. F. equiseti on PDA forms fast-growing, woolly colonies with salmon to reddish-brown coloration. After successful amplification using PCR, producing distinct bands corresponding to approximately 583 bp and 576 bp for the first and second isol.....

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