AbstractA study was conducted to construct a preliminary linkage map for molly, Poecilia and to test
association between markers and QTL traits. In I inkage map construction, three major steps were
involved. First is the development of mapping population. Background genetic information of the
parental species such as occurrence of triploidy and genetic variability were crucial before any
attempt to develop a mapping population. Triploid detection was carried out in this study by using
highly polymorphic microsatellite (SSRs) primer pairs. Ten SSRs primer pairs could clearly
differentiate among one, two and three alleles which could be used to infer different ploidy
levels. The presence of two alleles indicates a diploid at that particular locus and evidence of
three alleles could be inferred as triploid. If such association was direct, the occurrence of
triploidy in molly fish sampled in this study was extremely low and negligible......