This study aimed to investigate the association of polymorphisms of Interleukin-4 (IL- 4) and Interleukin-6 (IL-6) genes to the susceptibility and severity of type-2 diabetes mellitus (T2DM) and to evaluate the serum levels of these interleukins in patients with T2DM. A number of 90 individuals from Wasit province, Iraq inclusive of 64 patients with T2DM and 26 healthy individuals with normal fasting blood sugar as a control group. Enzyme-linked immunosorbent assay (ELISA) was used to measure the levels of IL-4 and IL-6 in sera from patients with T2DM. The allele-specific polymerase chain reaction (PCR) technique was used to investigate the polymorphism of the interleukins: IL4 -590C/T, (rs2243250) and IL6 -174G/C, (rs1800795) genes polymorphisms. Results of this study showed that IL-4 concentrations had a non-significant difference (p = >0.052) between patients with T2DM (154.48 pg/ml ± 7.00) with the control group (151.49 pg/ml ± 6.21). Patients with T2DM revealed an ele.....

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Type-2 diabetes mellitus is the most common chronic metabolic disorder characterized by elevation of blood glucose concentration (hyperglycemia) due to defect in carbohydrate, lipid and protein metabolism. Cytokines have an important role in impairing insulin signaling and selective destruction of insulin-producing beta cells. Thus, the second type of diabetes can be characterized as an immune-mediated disease. This study aimed to evaluate the serum levels of Interleukin-4 (IL-4) and Interleukin-6 (IL-6) of patients with type 2 diabetes mellitus among cases from Wasit province-Iraq. A total of 90 randomly selected subjects from Wasit province ? Iraq: (60) patients with T2DM, and (30) apparently healthy subjects with normal fasting blood sugar as a control group. Enzyme-linked immunosorbent assay (ELISA) was used to measure the levels of interleukin-4 IL-4 and interleukin-6 in sera from patients with type-2 diabetes mellitus. The results of this study showed that IL-4 concentrations had.....

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