This research aims to enrich anaerobic ammonium oxidation (anammox) culture which capable of oxidizing ammonium to dinitrogen gas in a laboratory-scale anaerobic up-flow biofilm column reactor. A 16S rDNA gene analysis targeting planctomycetes-anammox bacteria was performed to screen anammox microorganism from sludge samples obtained from five sources of wastewater around Perak and Kuala Selangor. Anammox enrichment was carried out for 180 days in 1.0 L anaerobic up-flow biofilm column reactors with three different mode of feeding; (i) batch, (ii) fed-batch and (iii) continuous. The N-NH₄⁺, N-NO2ֿ and N-NO3ֿ concentrations were monitored throughout the enrichment period. The enriched anammox was identified using 16S rDNA and fluorescence in situ hybridization (FISH) techniques. After amplification using primer pair Pla46F-Amx368R, it was found that the genomic DNA from sludge of Jeram sanitary landfill showed a similarity in 16S rDNA gene to uncultured anammox bacteria clone AMX-.....

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