AbstractIn this study, new members of alkyl substituted thiourea derivatives of (RC(O)NHC(S)NHCH2CH(CH3)NHC(S)NHC(O)-R) in which R represents alkyl group namely isobutyryl and pivaloyl were successfully synthesized for their antibacterial activities. The compounds were spectroscopically and analytically characterized via infrared (IR) spectroscopy, ultraviolet–visible (UV– vis) spectroscopy, CHNS elemental analysis as well melting point analysis. In turn, they were screened for antibacterial activity using selected bacteria namely, Micrococcus sp., Salmonella typhi, Aeromonas sp., Klebsiella pneumoniae, Bacillus cereus and also Escherichia coli. From the antibacterial test, all synthesized compounds were found to exhibit great potential results as antibacterial agents, except for 1 which did not show inhibition activity towards Klebsiella pneumoniae. Thus, these proposed molecular structures have given ideal indication to act as antibacterial agents which have utmost potential in pharmaceu.....