This study aims to examine phycoremediation and biomass production of local microalgae isolates cultivated in high nitrate concentration of nitrified landfill leachate (NLL). The microalgae samples were collected from landfill leachate treatment plant and were isolated as potential species that are able to grow in high nitrate concentration. The isolates were identified using APHA's Key for Identification of Freshwater Algae and 16S rRNAl18S rDNA molecular techniques. Maximum microalgae growth, nitrate removal performance, and biomass productivity were determined at NLL concentration of 10 30% v/v. Biomass produced was further characterized for carbohydrate, lipid, and protein composition. The findings indicated that three species of local microalgae were successfully isolated namely; Chlorella sp., Scenedesmus sp. and Oscillatoria sp. with highest specific growth rate (11) of 0.62 day" (15% NLL), 0.71 day" (10% NLL), and 0.94 day" (10% NLL), respectively. Nitrate was removed by all mi.....

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This study aims to investigate the effect of photo-autotrophic cultural conditions (light intensity, temperature, pH, CO2 and NO3-) of C. vulgaris UPSI-JRM01 and to optimise the lipid, carbohydrate and biomass productivities for high biofuel feedstock production. In addition, carbon partitioning mechanism under nitrogen stress was also elucidated. The method of single-factor experiment was used to determine the effect of each cultural condition. The optimisation process was performed using statistical method of Plackett-Burman Design and Central Composite Design. The whole transcriptome analysis of gene expression under nitrogen stress was also performed using RNA sequencing. The results indicated that the lipid and carbohydrate yields were increased 3.19-fold and 1.39-fold under nitrogen stress and 5% CO2, respectively. The highest biomass productivity was achieved at 10,500 lux, 28 oC, pH 8, 5% CO2 and 500 mg/L NO3-. Meanwhile, the highest lipid yield was achieved at 23,500 lux, 40 o.....

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