This study aims to investigate the molecular epidemiology and risk factors of Entamoeba histolytica, Entamoeba dispar, and Entamoeba moshkovskii infection among Orang Asli school children in Perak. Stool samples were collected from 544 school children and the DNA extracted were amplified using nested multiplex PCR. The univariate and multivariate regression analyses were then used to determine the risk factor associated with Entamoeba species infection. The overall prevalence of Entamoeba complex infections was 21.3% (116/544). The total of school children infected with E. moshkovskii was 10.7% (58/544) followed by 9.0% (49/544) of E. dispar and 5.0% (27/544) of E. histolytica. One sample was positive for the mixed E. histolytica and E. dispar infection, five samples were positive for the mixed E. histolytica and E. moshkovskii infection, and 12 samples were positive for the mixed E. dispar and E. moshkovskii infection. As a result of the univariate analysis, there were 19 .....

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