Zingiber zerumbet (L.) Roscoe ex Sm. is a herbaceous medicinal plant from the Zingiberaceae family. Previous studies have mainly focused on the essential oils of the plant with little attention to its secondary metabolites. LCMS-Orbitrap is used to analyze ethanolic extract of Z. zerumbet rhizome. Dereplication is a technique applied to identify chemical constituents based on their accurate mass and comparison with custom databases, Dictionary of Natural Products (DNP), and literature using an operating system named MZmine 2.53. In this study, we have identified chemical constituents related to several classes, including flavonoids, terpenoids, diarylheptanoids, terpenes, phenolic compounds, and fatty acids. Our results provide useful information for further isolation and identification of other unknown compounds and mass spectroscopy-based metabolite profiling of Zingiber species...

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