Brassica oleracea var. italica is an important vegetable crops mostly grown in Cameron Highland, Malaysia. These vegetables are highly vulnerable to high temperature and grow best in cool climate. The purpose of this research was to examine in vitro growth response of B. oleracea var. italica through tissue culture system. Complete plant regeneration was successfully achieved when root, stem and leaf aseptic explant was cultured on MS medium supplemented with various combination of plant growth regulators. The experimental design used in this study was Completely Randomized Design (CRD). MS medium supplemented with 1.0 mg/L IBA + 0.5 mg/L BAP was found to be the optimum medium for shoot regeneration with 5.367±0.768 shoots per explant. Meanwhile 2.0 mg/L NAA was the optimum medium for leaf and root explant for root induction producing 7.567±0.498 and 8.767±0.707 roots per explant. Optimum callus induction was obtained when leaf explants were cultured on MS medium supplemented with 1.....

102 hits