AbstractThis study aimed to develop and test the usability of the digital comic _Mitoflip_ for subtopic mitosis for form 4 Biology students. The design of this study is developmental based on the ADDIE Model and the data of this study is analysed quantitatively. A total of 35 biology students from two secondary schools in Kinta district, Perak were involved as the research respondents. The questionnaire are used as instrument to obtain the usability of the digital comic comprised 30 items with a four-point Likert scale. The study data were analysed descriptively using the Statistical Packages for Social Science (SPSS) software version 26.0. The study findings show that the digital comic _Mitoflip_ has high face, content and item validity values, with CVI = 0.97. The Cronbach_s Alpha coefficient value also indicates good reliability, at _ = 0.81. Meanwhile, the findings for each construct, namely usefulness, ease of use, design, and satisfaction, obtained mean values (standard deviation) of 3.6.....