The purpose of this study was to examine the ability of seed germination Ocimum basilicum L. to germinate through tissue culture, the rate of time difference in seed germination in traditional or modern technology. In addition, callus formation was also carried out in the study to see the rate of cell differentiation at the exploratory part. At the end of the study, it was also tested by acclimatization of the explant to green house. The complete regeneration of the Ocimum basilicum L. plant and callus production was successfully produced through the tissue culture technique. The part of leaves, stems and roots of 8-week aseptic seedlings were used in this experiment. All cultures are stored at a temperature of 25 ± 1ºC and illuminated with fluorescent light for 16 hours of light, 8 hours dark. Plant acclimatization is done on three different soil types, namely garden soil, coconut powder and vermiculation. After completion, all data was recorded and analyzed using ANNOVA......

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