AbstractThis study aims to analyze the effect of Learning Organization (LO) on Innovative Work Behaviour (IWB) among Technical and Vocational Education and Training (TVET) teachers in vocational colleges across Malaysia, with Organizational Culture (OC) as a mediating variable. A quantitative approach using stratified simple random sampling was employed to analyze data from 331 TVET teachers. The questionnaire instrument was adapted from the Dimensions of Learning Organization Questionnaire, Organizational Culture Inventory, and Innovative Work Behaviour Questionnaire. Descriptive statistics showed high levels of LO (M = 3.91, SD = 0.63), OC (M = 3.94, SD = 0.67), and IWB (M = 3.99, SD = 0.72). Independent sample t-test and ANOVA revealed no significant differences in IWB based on gender [t(329) = -0.565, p = 0.57] and teaching experience [F(3,327) = 0.08, p = 0.92]. Pearson correlation analysis indicated significant positive relationships between LO and IWB (r = 0.58, p < 0.05), OC and IWB (r.....