AbstractExperiment were carried out to establish the most optimum culture condition and to
identify the most responsive explants for regeneration Brassica oleraceae L. var
Capitata through plant tissue culture system. Acclimatization process for established
plantlets was also conducted. The experimental design of this study was Complete
Randomized Design (CRD) method. For shoot regeneration using different
concentrations of BAP and NAA, the most responsive explant was stem explants in
which MS medium supplemented with 1.5 mg/L BAP and 0.5 mg/L NAA produced
8.400 ± 0.889 shoots per explant. Whereas, the combination auxin and cytokinin also
showed stem explants was the most responsive. Subsequently, for root regeneration
using different concentrations of BAP and NAA, the most responsive explant was
leave in which MS media supplemented with 0.5 mg/L BAP and 0.5 mg/L NAA
produced 51.133 ± 2.929 roots per explant. While the combination of auxin and
cytokinin showed the most responsive.....