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9 record(s) found

Characterization of major allergens of macrobrachium rosenbergii and their thermostability in different cooking methods

Abstract

Prawn allergy is a common cause of allergic reactions in countries where crustacean is a famous seafood. Macrobrachium rosenbergii (giant river prawn) is declared as a local major food allergens, causes severe symptoms like asthma and anaphylactic shock.Therefore, the goal concerning this research is to identify the allergenicity of heat treated of M. rosenbergii. Prawn extracts have been prepared using fresh raw prawn and three heat-treated prawn flesh (boiled, steamed, fried), then afterward analyzed by the usage of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Allergenic proteins were identified by means of immunoblotting by the use of sera from 30 prawnallergic patients. The identities of selective major allergens were then determined by massspectrometry analysis. The raw prawn has 27 protein fractions between 6 to 207 kDa, while the heat-treated prawns have reduced number of protein bands. Six prominent bands at 72, 65, 48, 38, 36, and 30 kDa were considere.....

Article Published 2019 3142 hits Limited

Identification Of Ige-Binding Proteins Of Male and Female Scylla Serrata (Mud Crab) Preliminary Results

Abdul Rahman Nurul Izzah, Misnan Rosmilah, Mohd Yadzir Zailatul Hani,

Abstract

Crab represents valuable nutrient source of protein, nutrients and functional lipids, is widely consumed. However the prevalence of crab allergy has increased over the past few years. In order to better understand local crab allergy, it is necessary to identify crab allergens. Multiple crab allergens including tropomyosin and arginine kinase have been identified as major allergens in various species of crab. Scylla serrata (mud crab) is an economically important crab species found in the Indo-Pacific region. So, the aim of this study was to identify the IgE-binding proteins of male and female Scylla serrata (mud crab). The raw extracts were prepared from the crab meat. The protein profiles and IgE-reactivity pattern were then identified by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting technique using sera from 10 patients with mud crab allergy. SDS-PAGE analysis of the male and female crab extracts revealed 19 protein bands ranging from 12 to .....

Conference Item Published 2012 4024 hits Available

Identification of major and minor allergens of Black Tiger Prawn (Penaeus monodon) and King Prawn (Penaeus latisulcatus)

Sahabudin Syuhaidah, Misnan Rosmilah, Mohammad Yadzir Zailatul Hani, Mohamad Jamaludin,

Abstract

Background: Prawns and shrimp are a frequent cause of seafood allergy mediated by IgE antibodies. Penaeus monodon and Penaeus latisulcatus, commonly known as black tiger prawn and king prawn, respectively, are among the most frequently consumed prawns in Malaysia. The aim of thi s study was to identify the IgE-binding proteins of these 2 prawn species. Methods: Raw and boiled prawn extracts were prepared and then resolved by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). IgE-immunoblotting was then performed using sera from patients with positive skin prick tests to the raw prawn extracts. Results: SDS-PAGE analysis of the raw extracts of both prawn species revealed 23 protein bands; the boiled extracts yielded fewer protein bands. The bands in the range of 40 to 100 kDa were sensitive to heat and therefore were not found in the boiled extracts. Immunoblot of raw extracts of black tiger prawns and king prawns yielded 14 and 11 IgE-binding proteins, respectively,.....

Article Published 2011 2158 hits Available

Identification of tropomyosin and arginine kinase as major allergens of Portunus pelagicus (blue swimming crab)

M Rosmilah, M Shahnaz, H.M.Y Zailatul, A Noormalin, I Normilah,

Abstract

Crab is an important source of food allergen. Tropomyosin represents the main crab allergen and is responsible for IgE cross-reactivity between various species of crustaceans. Recently, other new crab allergens including arginine kinase have been identified. However, information on allergens of the local Portunidcrab is not available. Thus, the aim of this study was to identify the major allergens of Portunus pelagicus (blue swimming crab) using the allergenomics approach. Raw and cooked extracts of the crab were prepared from the crab meat. Protein profile and IgE binding pattern were demonstrated by sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting using sera from 30 patients with crab allergy. The major allergens of the crab were then identified by two-dimensional electrophoresis (2-DE), followed by mass spectrometry analysis of the peptide digests. The SDS-PAGE of raw extract revealed approximately 20 protein fractions over a wide molecular weig.....

Article Published 2012 1218 hits Available

Immediate hypersensitivity to marine snail (cerithidea obtusa) allergen among local patients with atopic diseases

Salahudin Abd Aziz Norazlin, Misnan Rosmilah, Mohamad Yadzir Zailatul Hani, Bakhtiar Faizal, Abdullah Noormalin, Murad Shahnaz,

Abstract

Snail allergy is rare, but it can lead to severe allergic reactions. Life-threatening reactions including anaphylactic shock have been often reported after snail consumption. Cerithidea obtusa is a commonly consumed sea snail in Malaysia and other Asian countries. The purpose of this study was to determine the frequency of immediate hypersensitivity reactions to this species of snail by skin prick test (SPT). Snail proteins were extracted from the snail flesh. A total of 131 patients with atopic diseases were skin prick tested with the snail extract. Out of 131 subjects, 10 (8%) were sensitized to C. obtusa extract. Most of these subjects are allergic rhinitis (70%), allergic conjunctivitis (60%), asthma (20%) and food allergic (20%) patients. As a conclusion, this study showed that the immediate hypersensitivity to marine snail, C. obtusa exists among local atopic patients at a low frequency of 8%. It seems like a patient with C. obtusa sensitization is also probably to be sensitized .....

Article Published 2016 2556 hits Available

Identification of tropomyosin and troponin c as the major allergens of perna viridis (asian green mussel) and the effect of cooking on their allergenicity

Yadzir Zailatul HM, Misnan Rosmilah, Bakhtia Faizal, Abdullah Noormalin, Murad Shahnaz,

Article Published 2017 1999 hits Limited

Molecular and allergenic characterization of recombinant tropomyosin from mud crab Scylla olivacea

Rosmilah Misnan

Abstract

Background: Tropomyosin is a major allergen in crustaceans, including mud crab species, but its molecular and allergenic properties in Scylla olivacea are not well known. Thus, this study aimed to produce the recombinant tropomyosin protein from S. olivacea and subsequently investigate its IgE reactivity. Methods and Results: The tropomyosin gene was cloned and expressed in the Escherichia coli system, followed by SDS-PAGE and immunoblotting test to identify the allergenic potential of the recombinant protein. The 855-base pair of tropomyosin gene produced was found to be 99.18% homologous to Scylla serrata. Its 284 amino acids matched the tropomyosin of crustaceans, arachnids, insects, and Klebsiella pneumoniae, ranging from 79.03 to 95.77%. The tropomyosin contained 89.44% alpha-helix folding with a tertiary structure of two-chain alpha-helical coiled-coil structures comprising a homodimer heptad chain. IPTG-induced histidine tagged-recombinant tropomyosin was purified at the size of.....

Article Published 2021 1273 hits Available

Identification of major and cross-reactive allergens of local freshwater snail (Pila polita) and the impact of thermal and non-thermal food processing on allergen stability

Rosmilah Misnan

Abstract

BACKGROUND: Snail allergy is rare but can be fatal. Pila polita, a freshwater snail, was considered as a popular exotic food, particularly in tropical countries, and consumed in processed forms. Thus, the purpose of this study was to identify the major and cross-reactive allergens of P. polita and to determine the impact of food processing on the allergen stability. RESULTS: Sodium dodecyl sulfatepolyacrylamide gel electrophoresis fractionated raw snail extract to approximately 24 protein bands, between 9 and 245 kDa. The prominent band at 33 kDa was detected in all raw and processed snail extracts. Immunoblotting tests of the raw extract demonstrated 19 immunoglobulin E (IgE)-binding proteins, and four of them, at 30, 35, 42 and 49 kDa, were revealed as the major IgE-binding proteins of P. polita. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry identified the 49 and 42 kDa major allergens as actin, whereas the 30 and 35 kDa major allergens were identified .....

Article Published 2023 480 hits Available

Characterization the allergenicity and stability of allergens of freshwater snail, pomacea canaliculata

Rosmilah Misnan

Abstract

Snail allergy is considered a serious form of food allergy with prevalence of 1.4 to 22% worldwide. However, allergy to Pomacea canaliculata, a commonly consumed local snail has not been well-described. Hence, this study aimed to characterize the allergenicity and stability of the allergenic proteins of P. canaliculata by proteomics approach. Snail flesh was treated with several thermal and non-thermal treatments prior to overnight protein extraction. The snail proteins were then subjected to SDS-PAGE, followed by immunoblotting, two-dimensional electrophoresis (2-DE), 2-DE immunoblotting and mass-spectrometry analysis. Raw snail demonstrated 31 protein bands between 10 to 250 kDa, with fewer protein bands in treated snails. Boiled snails had the most protein bands among thermally treated snails, while salted and dried snails showed more bands than pickled snails among non-thermal treatments. Immunoblotting of raw extract demonstrated 16 IgE-binding bands, with the 33 and 42 kDa protei.....

Article Published 2024 334 hits Available

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