This research was carried out to investigate the factors that influence the productive performance of crossbreeding in goats under controlled feeding and managing. A total of 205 goats from Al Hilmi Agrofarm, Slim River, Perak were used in this study. These goats comprised of three indigenous breeds, namely, Katjang, Boer, and Jamnapari, and their hybrids. Sixty female goats were divided into 2 groups; treatment group (n = 30) which received Controlled Internal Drug Release (CIDR) for 9 consecutive days and artificial insemination was done on the 10th day, and a control group (n = 30) with normal breeding. Adult goats and their kids from each groups were weighed weekly and blood samples were withdrawn in every 2 weeks. Blood samples were collected into two types of tubes; containing ethylenediaminetetraacetic acid (EDTA) for hematological studies and without EDTA for protein profiling and gene polymorphism. ANOVA and t-test were used to see if there is any significant differences on st.....

1206 hits

This study aimed to identify the major allergens of two species of snails, Cerithidea obtusa (C. obtusa) and Pila polita (P. polita). C. obtusa and P. polita samples were obtained from Perak and Kelantan, respectively.Subsequently, the cross-reactive allergens between C. obtusa and P. polita with common local shellfish including Penaeus monodon (P. monodon), Loligo edulis (L. edulis) dan Scylla serrata (S. serrata) were identified. Proteins of C. obtusa, P. polita, P. monodon, L. edulis and S. serrata were extracted from their flesh. Protein profiles were determined by sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE). Major and cross-reactive allergens were then identified by immunoblotting and immunoblotting inhibition tests, respectively using sera from patients with allergy to C. obtusa, P. polita, P. monodon, L. edulis dan S. serrata. The major allergens of C. obtusa and P. polita were then identified by mass spectrometry analysis. Research findings showed that C.....

683 hits

This study aimed to produce allergen extracts, determine the frequency and characterize the major and cross-reactive allergens of five species of local bivalves. Allergen extracts were produced from raw and cooked bivalves. The raw bivalve extracts were used in skin prick tests (SPT) on 131 atopic patients. Protein profiles, major and cross-reactive allergens were then identified by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), immunoblotting and immunoblotting inhibition tests, respectively, using sera from bivalve-allergic patients. The major allergens were then identified by mass spectrometry analysis. Research findings indicated that SDS-PAGE of raw extracts revealed 12 to 23 protein bands between 13 to 250 kDa, while the cooked extracts have fewer protein bands. 34 (26%) of the subjects had a positive SPT to at least one bivalve species. The frequency of SPT reactivity to Anadara granosa was the highest at 18%, while Perna viridis was the lowest at 5%. Immu.....

1062 hits

941 hits

1399 hits

1313 hits

4230 hits

1560 hits

1540 hits

1363 hits