This study aims to characterize the allergenic properties and determine the biochemical stability of allergens of purple mud crab, Scylla tranquebarica. Raw extracts were  prepared from the muscle tissues of both mixed and specific body parts. Various treated extracts  were prepared from the muscles of mixed body parts. Protein profiles, allergenic proteins and  cross-reactive allergens were then identified by sodium  dodecyl  sulfate-polyacrylamide  gel   electrophoresis  (SDS-PAGE), immunoblotting and immunoblotting inhibition test techniques,  respectively, using sera from crab-allergic patients. The identification of major allergens were  analyzed by mass spectrometry. This study showed that raw mixed part extract contains the most  protein bands and allergenic proteins than the specific body parts and all treated extracts. Five  major allergens were identified at 38, 42, 50, 63 and 73 kDa. Mass spectrometry analysis identified  the 42 and 50 kDa as argi.....

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