AbstractThe purpose of this study was to examine the propagation ability of Punica granatum
L. through tissue culture system. In addition, callus induction was also carried out in the study
to observe the rate of cell differentiation on explants. The experimental design that was used in
this research is Completely Randomized Design (CRD). The complete regeneration of the Punica
granatum L. plant and callus production was successfully produced through the tissue culture
technique. Explants used in this study were leaves, stem and roots from 8-week-old aseptic
seedlings. All cultures were stored at a temperature of 25 ± 1ºC and light 16 hours of light,
8 hours dark. Plant acclimatization was done on three different medium, garden soil, coco peat and
vermiculite. All data was recorded and analyzed using ANOVA. Leaf explants were found to
be very responsive and Murashige and Skoog (MS) medium add.....