The purpose of this study was to examine the propagation ability of Punica granatum L. through tissue culture system. In addition, callus induction was also carried out in the study  to observe the rate of cell differentiation on explants. The experimental design that was used in  this research is Completely Randomized Design (CRD). The complete regeneration of the Punica  granatum L. plant and callus production was successfully produced through the tissue culture  technique. Explants used in this study were leaves, stem  and  roots  from  8-week-old  aseptic   seedlings.  All  cultures  were  stored  at  a temperature of 25 ± 1ºC and light 16 hours of light,  8 hours dark. Plant acclimatization was done on three different medium, garden soil, coco peat and  vermiculite. All data was  recorded  and  analyzed  using  ANOVA.  Leaf  explants  were  found  to   be  very responsive   and   Murashige   and   Skoog   (MS)   medium   add.....

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