Plants of the genus Piper have long been used as medicinal herbs. The chemistry of Piper species has been widely investigated, and phytochemicals investigations led to the isolation of a number of physiologically active compounds. This study was carried out to investigate the phyto - chemicals isolated from the leaf and stem extracts of Piper ornatum. Cold extraction of the dried powdered leaf and stem was employed to extract the phytochemicals according to the polarity gradient of hexane, ethyl acetate, and methanol. The phytochemicals were obtained using chromatography techniques (column chromatography and prep-TLC) and their structures were confirmed by spectroscopic analysis (IR, NMR and MS) and comparison with literature. The isolation was successfully led to the identification of 5,7-dimethoxyflavone (1), 4',7-dihydroxy-3',5',5-trimethoxyflavone (2), 4'-hydroxy-3',5',5,7-tetramethoxyflavone (5) and 3',4',5,5',7-pentamethoxyflavone (6), together with piperine (3) and ?-sitosterol .....

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The purpose of the study was to investigate phytochemicals, essential oils, and antioxidant activity from Piper ornatum N.E.Br and Piper penangense C.DC (Piperaceae). The essential oils were obtained by hydrodistillation technique and their compositions were evaluated by gas chromatography (GC-FID) and gas chromatography-mass spectrometry (GC-MS). Isolation of phytochemicals was carried out through cold extraction of the leaves followed by separation using chromatographic techniques. The isolated compounds were confirmed by spectroscopic data and comparison with literature. The antioxidant activity of the essential oils, crude extracts, and phytochemicals were evaluated using the total phenolic content (TPC) and DPPH free radical scavenging assays. The study showed that the essential oil of P. ornatum consists of caryophyllene oxide (31.5%), sphatulenol (5.9%), aromadendrene (4.9%), and _-caryophyllene epoxide (4.5%). The major components of P. penangense essential oil were.....

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