The quality of indoors in university buildings is vital to ensure a conducive learning environment and health and well-being. The objectives of this study are to investigate microbial aerosols and the concentration of particulate matter (PM2.5) in a university building. The data was collected from three lecturers’ rooms (Room A, B and C). The microbial aerosol was collected using a microbial air sampler, while Total Bacterial Count (TBC) and Total Fungi Count (TFC) were counted to analyze the colony-forming unit. In addition, PM2.5 was obtained using a low-volume air sampler (LVS), for five hours. The Field-Emission Scanning Electron Microscope (FESEM) was used to analyze the morphological composition of indoor PM2.5. The findings indicated that Room A shows the highest concentration of TBC (251.43 ± 32.74 cfu m-3) and TFC (28.57 ± 2.65 cfu m-3) compared to the two other rooms. In addition, the TBC showed a significantly higher amount (p..

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