AbstractThis research was conducted to prepare, characterise and determine the effectiveness of nanoemulsion formulations consisting of biopolymers and plant essential oils. Anionic tragacanth gum and cationic guar gum act as nanocarriers for betel, clove and lemongrass essential oils, which are active ingredients in nanoemulsion systems. The characterisation studies were carried out using Fourier transform infrared (FTIR) spectrometer, thermogravimetric analyser (TGA), differential scanning calorimeter (DSC), high resolution transmission electron microscope (HR-TEM), field emission scanning electron microscope (FESEM), zeta potential and particle size analyser. The ability of nanoemulsion formulations to load and release essential oils in vitro in rainwater was determined using an ultraviolet-visible (UV-Vis) spectrophotometer. The Excito chamber study was performed to evaluate the effectiveness of the fabrics treated with nanoemulsion formulations to repel Aedes aegypti mosquitoes. Based on .....