The aim of the present study was to extract of Polyalthia sclerophylla leave (LPS) and identify its chemical constituents using Gas chromatography-Mass (GS-Mass) spectrometry and classical phytochemicals methods. This study included as well the evaluated of the bio-medical properties of LPS using antibacterial (against six bacteria species) and cytotoxicity (M-63 human cell line) activities. Three different solvent system i.e. methanol (MeOH), dichloromethane (DCM) and hexane were used to extract the LPS, three samples were obtained and labelled as MLPS, DLPS, and HLPS, respectively, and evaluated their biomedical activities. Phytochemical screening results showed to present glycosides and terpenoids in MLPS, DLPS, and HLPS, while alkaloids did not detect the presence in all extracts. GC-Mass results were detected to present 21 chemical compounds, the higher percentages were cyclobutanone, 2-methyl-2-oxiranyl-, 2-Undecanol and Pyridine, 2,3,4,5-tetrahydro-3-methyl-, while 1,6-Heptadien.....

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This study was amid to biogenic synthesis of Silver Nanoparticles (AgNPs) and evaluated its chemical-physical and biomedical properties. The currently used reducing agent is Polyalthia sclerophylla (CEPS). As a reducer, a crude extract of Polyalthia sclerophylla leaves (CEPS) was used, while silver nitrate (AgNO3) was used as an initiator. Two samples were prepared and named AgNPs-a and AgNPs-b, respectively. The prepared samples were carried out to characterize their biological, physical, and chemical properties. Energy Dispersive X-Ray Analysis (EDX) and ultraviolet-visible spectroscopy were the first techniques utilised to emphasise the formulation of AgNPs (Uv-vis). The morphology and size of the particles are determined using scanning transmission electron microscopy (STEM) and scanning electron microscopy (SEM). AgNPs were tested for cytotoxicity against Mg-63 human cells (a type of osteosarcoma cell and its osteoblast-like cells) using the Alamar blue assay, and their antibacter.....

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The present study was conducted to prepare the fish scales' hydroxyapatite/collagen beads (FsHA/FsCol) and characterize their biological, physical, and chemical properties. A new method was used to prepare FsHA/FsCol composite beads by infiltrating the beads of FsHA in the solution of FsCol as a green method. X-ray diffraction analysis (XRD), scanning electron microscopy (SEM) analysis, Fourier-transform infrared (FTIR) spectroscopy analysis and energy dispersive X-ray analysis (EDX), used to evaluate the physical-chemical properties of the synthesized samples. Meanwhile, the cytotoxic and attachment studies of the FsHA/FsCol beads were used to investigate the biological features against the MG-63 human cell line. The results specified the efficiency of the new method, functional groups of FsCol were indicated to be present inside the beads of FsHA according to the XRD analysis which shows the functional peaks of FsCol. The SEM image were conformed successfully use starch as a porous a.....

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In this study, the properties of composites produced using solvent casting technique from poly(lactic acid) (PLA) and fish scale hydroxyapatite (FsHA) were investigated. Some amount of FsHAp powders (from 10 to 70 wt%) were added to the PLA solution and stirred for eight hours after the PLA was dissolved in the dichloromethane (DCM) solvent. The homogeneous mixture of PLA and FsHA was poured into the metal mould and dried in the oven for 4 hours to produce a dried PLA/FsHA composite sheet. The PLA/FsHA composite sheet with different FsHA filler contents was successfully prepared. These composites were characterised by Fourier transform infared spectroscopy (FTIR), differential scanning calorimetry (DSC), tensile tester and scanning electron microscope (SEM). It can be seen that by addition of 50 wt% FsHA filler contents gave the highest Youngs Modulus and tensile strength values of 2114.8 MPa and 23.3 MPa, respectively due to effective interaction between fillers and matrix. The meltin.....

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The utilization of ?-tricalcium phosphate (?-TCP) in medical field has been on demand for their excellent biocompatibility, bioactivity and solubility properties. This material can be produced through degradation of hydroxyapatite (HA) at high temperature. In this research, biogenic hydroxyapatite from fish scales (FsHA) was selected as pre-cursor for the synthesis of ?-TCP. The effect of yttria-stabilized zirconia (YSZ) addition on degradation of FsHA into ?-TCP have been investigated. Different amount of YSZ ranging from 5 to 15 wt% were mixed with FsHA and ball milled into fine powder. The mixture was then sintered at temperature of 1200 C. The materials were characterized using X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM). The results from FTIR, XRD and SEM-EDX analyses proved that the presence of ?-TCP in the sintered sample at 1200oC. The percentage of ?-TCP was calculated ranging from 17 to 21 % as analyzed fro.....

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Hydroxyapatite (HA) coatings on metallic implants have been extensively used in orthopedic applications to improve tissue-implant interactions, enhance their biocompatibility, and enhance their functionality. However, the expensive synthetic HA is the most widely used bioceramic for implant coatings, leading to high implants costs. Hence, this research explored the potential of an inexpensive biogenic HA derived from fish scales and FsHA/yttria-stabilized zirconia (YSZ) bioceramic coatings on a Ti6Al4V alloy as an alternative to synthetic HA coatings. The FsHA/YSZ powders and the coatings were examined with X-ray diffraction (XRD) and scanning electron microscopy/energy dispersive X-ray (SEM/EDX), and the surface roughness, microhardness, corrosion resistance, bioactivity, and in vitro cytotoxicity of the coatings were also determined. The morphological powder analysis revealed particles with a slightly irregular morphology and a fine spherical morphology, while the coating microstruct.....

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This work aimed to produce silver nanoparticles (AgNPs) by efficient green synthesis techniques, namely rapid green synthesis and modified microwave-assisted green synthesis methods. The study used fish scale collagen (FsCol) as a stabilizer to assess its impact on the dimensions and configurations of AgNPs. Four samples were prepared with varying concentrations of FsCol. The synthesized AgNPs were characterized using Ultraviolet–visible (UV–vis) spectroscopy, scanning electron microscope (SEM), energy dispersive X-ray analysis (EDX), Fourier Transform Infrared Spectroscopy (FTIR), X-Ray diffraction analysis (XRD), Dynamic Light Scattering (DLS), and Transmission electron microscopy (TEM) techniques. The obtained sizes are as follows: 85 ± 15 nm, 70 ± 10 nm, 50 ± 10 nm, and 28–40 nm. The UV–vis spectroscopy revealed a shift in the absorbance peaks from 400 to 446 nm. The SEM method showed a spherical form in all of the samples. The element silver was detected in the EDX exam.....

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Polyalthia rumphii (P. rumphii) is a member of the Annonaceae family with a wide distribution in tropical and subtropical regions. In traditional Chinese medicine, various members of this genus have been employed as medicinal plants to address refractory ailments. The Li ethnic minority, primarily residing in Hainan Island, has traditionally utilised extracts derived from P. rumphii for fever prevention, hypertension management, and the inhibition of cancer cell growth. The current study aimed to extract P. rumphii leaves and identify their chemical components using GS-MS and traditional phytochemical methods. This study evaluated the LPr's biomedical properties using antibacterial and cytotoxicity activities. Three solvents were employed to extract the LPr: hexane, dichloromethane (DCM), and methanol (MeOH). Prepared samples were collected and labelled as HLPr, DLPr, and MLPr, respectively, and their biomedical activities were assessed. The results of phytochemical screening showed th.....

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The unique properties of silver nanoparticles (AgNPs), such as their high effectiveness against bacteria, are considered one of the reasons to encourage researchers to find easier and non-toxic ways to prepare them. The present study has been documented to investigate and assess the chemical, physical, and biological characteristics of AgNPs produced by biogenic synthesis. The twigs of polyalthia sclerophylla (P. sclerophylla) were used as an initiator and silver nitrate (AgNO3) as a reducer. The prepared samples were named AgNPs-a and AgNPs-b. Physical–chemical approaches were employed to characterize the produced samples. AgNPs were tested for cytotoxicity against fibroblast cell line L929 using the Alamar blue assay, while the evolution of the antibacterial activities was against S. aureus and E. coli. According to the data, AgNPs-a had a wavelength of 434 nm, and AgNPs-b had a wavelength of 454 nm. SEM and STEM pictures revealed that the prepared samples had spherical forms. AgNP.....

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This study investigates biomaterial composites made from a poly(lactic acid) (PLA) polymer matrix and hydroxyapatite (HA) extracted from fish scales (FsHA) for biomedical applications, especially in 3D printing. Fish scale HA is considered a halal alternative to synthetic or bone-derived HA. The research aims to explore the chemical, mechanical, and thermal properties of PLA/FsHA composites. Samples were prepared by melt-blending PLA with varying FsHA weights (10-50 wt%) at 190 °C, followed by hot pressing. Mechanical properties were analyzed using tensile tests, while scanning electron microscopy (FESEM) characterized the morphology of fractured specimens. Fourier transform infrared spectroscopy (FTIR) studied the functional groups between PLA and FsHA. Thermal properties, including glass transition temperature (Tg) and melting temperature (Tm), were investigated using differential scanning calorimetry (DSC). Mechanical analysis revealed that optimal properties were achieved at 20 wt.....

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