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17 record(s) found

Factors impacting research productivity of academic staff at the Iraqi higher education system

Abstract

Research productivity is a vital element in enhancing the university ranking and, it is an important metric to measure the performance of academic staff. The purpose of this study is to identify the factors impacting research productivity of academic staff at Cihan University Erbil, Iraq. A survey was carried out among 87 academic staff randomly selected at the university to determine the impact of Fund, Collaboration, Information and Communication Technology (ICT), and Job Satisfaction on Research Productivity. The data was analyzed by using Smart PLS 3.2.9. The results indicated that Fund, Collaboration, ICT and Job Satisfaction had positive and significant impact on Research Productivity, although Fund has the highest impact on Research Productivity. The implication of this study is the management of universities should pay greater attention on research funding opportunities, rewarding collaboration among researchers, enabling ICT and improving job satisfac ion to boost research pr.....

Article Published 2020 2159 hits Available

An in vitro cytotoxicity of a novel pH-sensitive lectin loaded-cockle shell-derived calcium carbonate nanoparticles against MCF-7 breast tumour cell

Al-Obaidi, Jameel R.

Abstract

The acidic microenvironment of tumour cells requires a pH-sensitive delivery mechanism for improving drug release. In this study, we investigated the feasibility of using pH-sensitive ACC nanoparticles with sustained-release performance and slow degradability as carriers for lectins isolated from edible mushroom Agaricus bisporus (ABL) that exhibited anti-proliferating effects on tumour cells. ABL was purified and characterized using ion-exchange, gel filtration chromatography, sodium dodecyl sulfate-polyacrylamide gel electrophoresis and mass spectrometry. ABL-ACC nanoparticles were synthesized through a simple precipitation process coupled with mechanical grinding. The conjugated ABL-ACC-NPs were characterized using Fourier-transform infrared spectroscopy, X-ray powder diffraction, scanning electron microscope, transmission electron microscope and zeta potential. Cytotoxicity of ABL-ACC-NPs was analyzed using methylthiazol tetrazolium assay against MCF-7 cells compared with ABL and A.....

Article Published 2021 1788 hits Available

Detection of HMA5, PCS and MT2 genes expression in vicia faba under heavy metal stress using quantitative real-time pcr

Al-Obaidi, Jameel R.

Abstract

In order to achieve phytoremediation during agricultural production, it is essential to identify high genotypes yield that is able of accumulating many types of heavy metals not in the edible parts which have significant nutritional value but in the non-edible parts. This study conducted to estimate the heavy metal ATPases 5 (HMA5), Phytochelatins synthase (PCs) and metallothionein 2 (MT2) genes expression in plant Vicia faba in response to an elevated concentration of copper and zinc in nutrient media. Using Quantitative Real-Time PCR (RT-qPCR) technique, the results of hydroponic culture methods with high concentrations of copper (75 �Mol / L) and high concentration of zinc (500 � Mol / L) showed high expression level for the three genes of Vicia faba plant compared with control. Established that the expression of the genes under the influence of copper ion was higher than the expression under zinc ion influence. Besides that, gene expression increased with increased exposure tim.....

Article Published 2021 1479 hits Available

Aspergillus niger causing fruit rot disease on rose apple in Malaysia

Jameel Rabee Jameel Al-Obaidi

Abstract

[No abstract available]..

Article Published 2023 628 hits Available

LC-MS/MS proteomic study of MCF-7 cell treated with dox and dox-loaded calcium carbonate nanoparticles revealed changes in proteins related to glycolysis, actin signalling, and energy metabolism

Al-Obaidi, Jameel R.

Abstract

One of the most prevalent death causes among women worldwide is breast cancer. This study aimed to characterise and differentiate the proteomics profiles of breast cancer cell lines treated with Doxorubicin (DOX) and Doxorubicin-CaCO3-nanoparticles (DOX-Ar-CC-NPs). This study determines the therapeutic potential of doxorubicin-loaded aragonite CaCO3 nanoparticles using a Liquid Chromatography/Mass Spectrometry analysis. In total, 334 proteins were expressed in DOX-Ar-CC-NPs treated cells, while DOX treatment expressed only 54 proteins. Out of the 334 proteins expressed in DOX-CC-NPs treated cells, only 36 proteins showed changes in abundance, while in DOX treated cells, only 7 out of 54 proteins were differentially expressed. Most of the 30 identified proteins that are differentially expressed in DOX-CC-NPs treated cells are key enzymes that have an important role in the metabolism of carbohydrates as well as energy, including: pyruvate kinase, ATP synthase, enolase, glyceraldehyde-3-p.....

Article Published 2021 1400 hits Available

Mycopharmaceuticals and nutraceuticals: promising agents to improve human well-being and life quality

Al-Obaidi, Jameel R.

Abstract

Fungi, especially edible mushrooms, are considered as high-quality food with nutritive and functional values. They are of considerable interest and have been used in the synthesis of nutraceutical supplements due to their medicinal properties and economic significance. Specific fungal groups, including predominantly filamentous endophytic fungi from Ascomycete phylum and several Basidiomycetes, produce secondary metabolites (SMs) with bioactive properties that are involved in the antimicrobial and antioxidant activities. These beneficial fungi, while high in protein and important fat contents, are also a great source of several minerals and vitamins, in particular B vitamins that play important roles in carbohydrate and fat metabolism and the maintenance of the nervous system. This review article will summarize and discuss the abilities of fungi to produce antioxidant, anticancer, antiobesity, and antidiabetic molecules while also reviewing the evidence from the last decade on the impo.....

Article Published 2021 2115 hits Available

Protein profile of MCF-7 breast cancer cell line treated with lectin delivered by CaCO3NPs revealed changes in molecular chaperones, cytoskeleton, and membrane-associated proteins

Al-Obaidi, Jameel R.

Abstract

The second most predominant cancer in the world and the first among women is breast cancer. We aimed to study the protein abundance profiles induced by lectin purified from the Agaricus bisporus mushroom (ABL) and conjugated with CaCO3NPs in the MCF-7 breast cancer cell line. Two-dimensional electrophoresis (2-DE) and orbitrap mass spectrometry techniques were used to reveal the protein abundance pattern induced by lectin. Flow cytometric analysis showed the accumulation of ABL-CaCO3NPs treated cells in the G1 phase than the positive control. Thirteen proteins were found different in their abundance in breast cancer cells after 24 h exposure to lectin conjugated with CaCO3NPs. Most of the identified proteins were showing a low abundance in ABL-CaCO3NPs treated cells in comparison to the positive and negative controls, including V-set and immunoglobulin domain, serum albumin, actin cytoplasmic 1, triosephosphate isomerase, tropomyosin alpha-4 chain, and endoplasmic reticulum chaperone B.....

Article Published 2021 1292 hits Available

Uncovering prospective role and applications of existing and new nutraceuticals from bacterial, fungal, algal and cyanobacterial, and plant sources

Jameel Rabee Jameel Al-Obaidi

Abstract

Nutraceuticals are a category of products more often associated with food but having pharmaceuticals property and characteristics. However, there is still no internationally accepted concept of these food?pharmaceutical properties, and their interpretation can differ from country to country. Nutraceuticals are used as part of dietary supplements in most countries. They can be phy-tochemicals which are biologically active and have health benefits. These can be supplied as a sup-plement and/or as a functional food to the customer. For human health and longevity, these materials are likely to play a vital role. Consumption of these items is typical without a therapeutic pre-scription and/or supervision by the vast majority of the public. The development of nutraceuticals can be achieved through many bioresources and organisms. This review article will discuss the current research on nutraceuticals from different biological sources and their potential use as an agent for improving human he.....

Article Published 2021 2265 hits Available

Detection of orthodontically induced inflammatory root resorption-associated biomarkers from the gingival crevicular fluid by proteomics analysis: a randomized-controlled clinical trial

Al-Obaidi, Jameel R.

Abstract

Orthodontically induced inflammatory root resorption (OIIRR) is an undesirable complication of orthodontic treatment (OT) with an ambiguous aetiologic mechanism. This study aimed to identify OIIRR-associated biomarkers in the gingival crevicular fluid (GCF) using proteomic analysis. In this randomized clinical trial, the upper first premolars (UFP) were exposed either to light or heavy force. The GCF was collected at 1h, 1day, 7days, 14days, 21days, and 28days following force application. After extraction of UFP, roots were imaged and resorption premolar, was used to deliver either light forcecraters were measured. Proteomic analysis of GCF was performed using 2D gel electrophoresis with MALDI-TOF/TOF MS/MS. Results were further analyzed by bioinformatics analyses showing the biological functions and predicted pathways. The predicted canonical pathways showed that the expression of immunoglobulin kappa (IGKC), neutrophil gelatinase-associated lipocalin (NGAL), neurolysin mitochondrial .....

Article Published 2023 695 hits Available

Cross-reactivity analysis of milk proteins from different goat breeds with cow milk allergens using a proteomic approach

Al-Obaidi, Jameel R.

Abstract

Introduction: Goat's milk thought to be a good substitute for cow's milk protein allergic (CMPA) individuals. However, there is growing evidence that their proteins have cross-reactivities with cow's milk allergens. This study aimed to profile and compare milk proteins from different goat breeds that have cross-reactivity to cow's milk allergens. Methodology: Proteomics was used to compare protein extracts of skim milk from Saanen, Jamnapari, and Toggenburg. Cow's milk was used as a control. IgE-immunoblotting and mass spectrometry were used to compare and identify proteins that cross-reacted with serum IgE from CMPA patients (n = 10). Results: The analysis of IgE-reactive proteins revealed that the protein spots identified with high confidence were proteins homologous to common cow's milk allergens such as ?-S1-casein (?S1-CN), ?-casein (?-CN), ?-casein (?-CN), and beta-lactoglobulin (?-LG). Jamnapari's milk proteins were found to cross-react with four major milk allergens: ?-S1-CN, ?.....

Article Published 2023 624 hits Available

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